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  4. Recombinant Salmonella typhimurium Flagellar hook-associated protein 2 (fliD)

Recombinant Salmonella typhimurium Flagellar hook-associated protein 2 (fliD)

  • 货号：
    CSB-YP321436SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP321436SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP321436SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP321436SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP321436SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    fliD
  • Uniprot No.：
  • 别名：
    fliD; flaV; flbC; STM1960Flagellar hook-associated protein 2; HAP2; Filament cap protein; Flagellar cap protein
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-467
  • 氨基酸序列
    ASISSLGVG SNLPLDQLLT DLTKNEKGRL TPITKQQSAN SAKLTAYGTL KSALEKFQTA NTALNKADLF KSTVASSTTE DLKVSTTAGA AAGTYKINVT QLAAAQSLAT KTTFATTKEQ LGDTSVTSRT IKIEQPGRKE PLEIKLDKGD TSMEAIRDAI NDADSGIAAS IVKVKENEFQ LVLTANSGTD NTMKITVEGD TKLNDLLAYD STTNTGNMQE LVKAENAKLN VNGIDIERQS NTVTDAPQGI TLTLTKKVTD ATVTVTKDDT KAKEAIKSWV DAYNSLVDTF SSLTKYTAVE PGEEASDKNG ALLGDSVVRT IQTGIRAQFA NSGSNSAFKT MAEIGITQDG TSGKLKIDDD KLTKVLKDNT AAARELLVGD GKETGITTKI ATEVKSYLAD DGIIDNAQDN VNATLKSLTK QYLSVSNSID ETVARYKAQF TQLDTMMSKL NNTSSYLTQQ FTAMNKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.
  • 基因功能参考文献：
    1. The solution structure of the FliT chaperone in the free state and in complex with FliD and the flagellar ATPase FliI has been determined. PMID: 27528687
    2. Escherichia coli FliD consists of three domains (D1, D2, and D3) and forms a hexamer plate of the D2 and D3 domains that resembles a six-pointed star with legs consisting of the D1 domain. In contrast, the D2 and D3 domains of Salmonella typhimurium FliD assemble into a pentamer as a five-pointed star plate. Despite their distinct oligomeric states, ecFliD and stFliD engage a common molecular surface for oligomerization. PMID: 28179186
  • 亚细胞定位：
    Secreted. Bacterial flagellum.
  • 蛋白家族：
    FliD family
  • 数据库链接：

    KEGG: stm:STM1960

    STRING: 99287.STM1960