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  4. Recombinant Saccharomyces cerevisiae tRNA (His) guanylyltransferase

Recombinant Saccharomyces cerevisiae tRNA (His) guanylyltransferase

  • 中文名称：
    酿酒酵母THG1重组蛋白
  • 货号：
    CSB-YP347181SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母THG1重组蛋白
  • 货号：
    CSB-EP347181SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母THG1重组蛋白
  • 货号：
    CSB-EP347181SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母THG1重组蛋白
  • 货号：
    CSB-BP347181SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母THG1重组蛋白
  • 货号：
    CSB-MP347181SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    THG1
  • Uniprot No.：
  • 别名：
    THG1; YGR024C; tRNA(His) guanylyltransferase; EC 2.7.7.79; tRNA-histidine guanylyltransferase
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-237
  • 氨基酸序列
    ANSKFGYVR QFETHDVILP QCYIVVRIDG KKFHEFSKFY EFAKPNDENA LKLMNACAKN LVLKYKNDII LAFGESDEYS FILKSSTTLF NRRKDKLATL FGSFFTSNYV ALWAKFFPEK PLNIKHLPYF DSRCVAYPNL QTIKDYLSWR YVDTHINNLY NTTFWQLIIK CGLTPQESEK KLCGTFSNEK QEILFSECGI NYNNEPEMFK KGSLVTRKGE ILHINVIAQI DELFEGY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.
  • 基因功能参考文献：
    1. Minimal requirements for reverse polymerization and tRNA repair by tRNA(His) guanylyltransferase Thg1 have been presented. PMID: 28901837
    2. This study determined the crystal structure of GTP-bound Thg1 from Saccharomyces cerevisiae at the maximum resolution of 3.0 A. PMID: 28623126
    3. pyrophosphate removal versus nucleotide addition activities of yeast Thg1 with a set of tRNA(His) substrates PMID: 24548272
    4. Study provides the first kinetic insight into the individual steps of the non-Watson-Crick addition of G- to tRNAHis, the known physiological function of Thg1 in yeast. PMID: 22136300
    5. tRNA(His) from Thg1p-depleted cells is uncharged, although histidyl tRNA synthetase is active and the 3' end of the tRNA is intact, suggesting that G(-1) is a critical determinant for aminoacylation of tRNA(His) in vivo. PMID: 16135808
    6. tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases PMID: 16625026
    7. These results suggest that 3'-5' polymerization represents either an uncharacterized role for Thg1 in RNA or DNA repair or metabolism, or it may be a remnant of an earlier catalytic strategy used in nature. PMID: 16731615
    8. Thg1 substrate recognition is at least in part mediated by preventing recognition of incorrect substrates for nucleotide addition PMID: 18366186

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  • 蛋白家族：
    TRNA(His) guanylyltransferase family
  • 数据库链接：

    KEGG: sce:YGR024C

    STRING: 4932.YGR024C