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  4. Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1 (DCP1)

Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1 (DCP1)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1(DCP1),Yeast
  • 货号：
    CSB-YP622491SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1(DCP1),Yeast
  • 货号：
    CSB-EP622491SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1(DCP1),Yeast
  • 货号：
    CSB-EP622491SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1(DCP1),Yeast
  • 货号：
    CSB-BP622491SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 1(DCP1),Yeast
  • 货号：
    CSB-MP622491SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DCP1
  • Uniprot No.：
  • 别名：
    DCP1; YOL149WmRNA-decapping enzyme subunit 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-231
  • 氨基酸序列
    MTGAATAAEN SATQLEFYRK ALNFNVIGRY DPKIKQLLFH TPHASLYKWD FKKDEWNKLE YQGVLAIYLR DVSQNTNLLP VSPQEVDIFD SQNGSNNIQV NNGSDNSNRN SSGNGNSYKS NDSLTYNCGK TLSGKDIYNY GLIILNRINP DNFSMGIVPN SVVNKRKVFN AEEDTLNPLE CMGVEVKDEL VIIKNLKHEV YGIWIHTVSD RQNIYELIKY LLENEPKDSF A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast. It occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. DCP1 is activated by the DEAD-box helicase DHH1 and destabilizes the eIF-4F cap-binding complex from the mRNA.
  • 基因功能参考文献：
    1. Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition PMID: 21148770
    2. Some mutations located in the conserved motifs and at the interface between the two Dhh1 domains confer dominant negative phenotypes in vivo and disrupt the conformational switch in vitro. PMID: 15987810
  • 亚细胞定位：
    Cytoplasm, P-body. Note=Is concentrated in several cytoplasmic foci called P bodies (or cytoplasmic processing bodies) which represent sites of mRNA decapping and 5' to 3' exonucleotidic decay.
  • 蛋白家族：
    DCP1 family
  • 数据库链接：

    KEGG: sce:YOL149W

    STRING: 4932.YOL149W