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  4. Recombinant Saccharomyces cerevisiae Zuotin (ZUO1)

Recombinant Saccharomyces cerevisiae Zuotin (ZUO1)

  • 中文名称：
    酿酒酵母ZUO1重组蛋白
  • 货号：
    CSB-YP333706SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母ZUO1重组蛋白
  • 货号：
    CSB-EP333706SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母ZUO1重组蛋白
  • 货号：
    CSB-EP333706SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母ZUO1重组蛋白
  • 货号：
    CSB-BP333706SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母ZUO1重组蛋白
  • 货号：
    CSB-MP333706SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZUO1
  • Uniprot No.：
  • 别名：
    ZUO1; YGR285CZuotin; DnaJ-related protein ZUO1; J protein ZUO1; Heat shock protein 40 homolog ZUO1; Ribosome-associated complex subunit ZUO1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-433
  • 氨基酸序列
    MFSLPTLTSD ITVEVNSSAT KTPFVRRPVE PVGKFFLQHA QRTLRNHTWS EFERIEAEKN VKTVDESNVD PDELLFDTEL ADEDLLTHDA RDWKTADLYA AMGLSKLRFR ATESQIIKAH RKQVVKYHPD KQSAAGGSLD QDGFFKIIQK AFETLTDSNK RAQYDSCDFV ADVPPPKKGT DYDFYEAWGP VFEAEARFSK KTPIPSLGNK DSSKKEVEQF YAFWHRFDSW RTFEFLDEDV PDDSSNRDHK RYIERKNKAA RDKKKTADNA RLVKLVERAV SEDPRIKMFK EEEKKEKERR KWEREAGARA EAEAKAKAEA EAKAKAESEA KANASAKADK KKAKEAAKAA KKKNKRAIRN SAKEADYFGD ADKATTIDEQ VGLIVDSLND EELVSTADKI KANAAGAKEV LKESAKTIVD SGKLPSSLLS YFV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. ZUO1 can act as a J-protein for SSB1/SSB2 only when associated with SSZ1.
  • 基因功能参考文献：
    1. Ssz1 is catalytically inert and cannot adopt the closed conformation, but the substrate binding domain beta is completed by Zuo1. PMID: 28067917
    2. This study presents new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae ribosome-associated J protein-Hsp70 chaperone Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24. PMID: 27669034
    3. The yeast ribosome-associated complex is composed of HSP40 (Zuotin) and HSP70 (Ssz1). RAC stabilizes the 80S ribosome in nonrotated, classical conformation. PMID: 25362488
    4. study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; a unique alpha-helical domain in Zuo1 mediates ribosome interaction of RAC near the ribosomal proteins L22e and L31e and ribosomal RNA helix H59 PMID: 23202586
    5. Zuo1 activates Pdr1 causing premature growth arrest of cells during the diauxic shift, through quorum sensing, as they adapt to the changing environmental conditions. PMID: 22203981
    6. Both Jjj1 and Zuo1 associate with nuclear 60S ribosomal biogenesis intermediates and play an important role in nuclear rRNA processing, leading to mature 25S rRNA PMID: 20368619
    7. structural analysis of teh ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction between zuotin and ssz PMID: 19920147
    8. Zuo1 efficiently stimulates the ATPase activity of Ssb only when in complex with another Hsp70, Ssz1. PMID: 15908962
    9. A plausible role of the ZUO1 chaperone is to stabilize genetic networks, thus making them more tolerant to malfunctioning of their constituents. PMID: 16849597

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: sce:YGR285C

    STRING: 4932.YGR285C