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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 74 (VPS74)

Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 74 (VPS74)

  • 货号：
    CSB-YP313287SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313287SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP313287SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313287SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313287SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS74
  • Uniprot No.：
  • 别名：
    VPS74; YDR372C; D9481.15Vacuolar protein sorting-associated protein 74
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MSTLQRRRVN RADSGDTSSI HSSANNTKGD KIANIAVDGD DDNGTNKKIA YDPEESKLRD NINIPTLTLM EEVLLMGLRD REGYLSFWND SISYALRGCI IIELALRGKI RILDDSARKR FDLSERLIEV IDSSKTGEVL LDETLQLMKN DEPLSISNWI DLLSGETWNL LKINYQLKQV RERLAKGLVD KGVLRTEMKN FFLFDMATHP IADASCKEAI KRRVLSVLVS RNMELSYNEY FPETTSFKII RTLALICGSY GANVLENVLT TLEYEKRDKA ISRAEEIMAQ FSQYPFDLEK ETELGVSVNL NKEVKEEIEN NPGHDLQLEV IAGVFEVFSR MDMLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.
  • 基因功能参考文献：
    1. Disruption of the Sac1-Vps74 interface results in a broader distribution of phosphatidylinositol 4-phosphate within the Golgi apparatus and failure to maintain residence of a medial Golgi mannosyltransferase. PMID: 25113029
    2. propose that Vps74p may use different domains to interact with specific effectors thereby differentially modulating a variety of cellular functions PMID: 24019977
    3. Vps74 Arg-rich N terminus binds directly to coatomer. PMID: 22889169
    4. additive roles of Vps74-mediated and sphingolipid-dependent sorting of Golgi residents. PMID: 22553352
    5. Targeting of Vps74 to the Golgi apparatus requires ongoing synthesis of phosphatidylinositol (PtdIns) 4-phosphate (PtdIns4P) by the Pik1 PtdIns 4-kinase and that modulation of the levels and cellular location of PtdIns4P leads to mislocalization. PMID: 20026658
    6. Data show that yeast Vps74p directly binds to the cytosolic domains of cis and medial Golgi mannosyltransferases and that loss of this interaction correlates with loss of Golgi localization of these enzymes. PMID: 18410729
    7. it is proposed that Vps74p maintains the steady-state localization of Golgi glycosyltransferases dynamically, by promoting their incorporation into COPI-coated vesicle PMID: 18635803

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  • 亚细胞定位：
    Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Note=Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.
  • 蛋白家族：
    GOLPH3/VPS74 family
  • 数据库链接：

    KEGG: sce:YDR372C

    STRING: 4932.YDR372C