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  4. Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit E (VMA4)

Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit E (VMA4)

  • 货号：
    CSB-YP324354SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP324354SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP324354SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP324354SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP324354SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VMA4
  • Uniprot No.：
  • 别名：
    VMA4; VAT5; YOR332W; O6241; V-type proton ATPase subunit E; V-ATPase subunit E; V-ATPase 27 kDa subunit; Vacuolar proton pump subunit E
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-233
  • 氨基酸序列
    SSAITALTP NQVNDELNKM QAFIRKEAEE KAKEIQLKAD QEYEIEKTNI VRNETNNIDG NFKSKLKKAM LSQQITKSTI ANKMRLKVLS AREQSLDGIF EETKEKLSGI ANNRDEYKPI LQSLIVEALL KLLEPKAIVK ALERDVDLIE SMKDDIMREY GEKAQRAPLE EIVISNDYLN KDLVSGGVVV SNASDKIEIN NTLEERLKLL SEEALPAIRL ELYGPSKTRK FFD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • 基因功能参考文献：
    1. Glu-44 is one of the key functional residues for subunit interaction in the V-ATPase complex. PMID: 24196958
    2. Data indicate the interactions in V-ATPase Vma4 (subunit E) and Vma10 (subunit G). PMID: 23071676
    3. new structural details of subunit E are discussed in the light of the structural arrangements of this subunit inside the V- and VV ATPase. PMID: 21826517
    4. NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase. PMID: 21399923
    5. Mutational analysis of V-ATPase indicates that subunit E could communicate cellular changes to the catalytic sites upon binding to regulatory proteins which interact with subunit E as a means of regulating V-ATPase function. PMID: 15718227
    6. Protein interaction studies show that the N-terminal region of Vma4 (subunit E) is a key factor not only in the stator structure of the vacuolar ATPase rotary molecular motor, but also in mediating interactions with putative regulatory subunits. PMID: 15751969
    7. an increase in the level of the TIM23 translocase associated with the cytochrome bc1-COX supercomplex is observed in the absence of Su e and Su g PMID: 18187422
    8. These results suggest that subunit E, especially its amino-terminal domain, plays a pertinent role in the assembly of V-ATPase subunits in vacuolar membranes from mice and yeast. PMID: 18662668

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  • 亚细胞定位：
    Vacuole membrane; Peripheral membrane protein.
  • 蛋白家族：
    V-ATPase E subunit family
  • 数据库链接：

    KEGG: sce:YOR332W

    STRING: 4932.YOR332W