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  4. Recombinant Saccharomyces cerevisiae Tubulin gamma chain (TUB4)

Recombinant Saccharomyces cerevisiae Tubulin gamma chain (TUB4)

  • 中文名称：
    酿酒酵母TUB4重组蛋白
  • 货号：
    CSB-YP347410SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TUB4重组蛋白
  • 货号：
    CSB-EP347410SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TUB4重组蛋白
  • 货号：
    CSB-EP347410SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TUB4重组蛋白
  • 货号：
    CSB-BP347410SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TUB4重组蛋白
  • 货号：
    CSB-MP347410SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TUB4
  • Uniprot No.：
  • 别名：
    TUB4; YLR212C; L8167.21; Tubulin gamma chain; Gamma-tubulin
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-473
  • 氨基酸序列
    MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT KPFFRENSRN KFTPRAIMMD SEPSVIADVE NTFRGFFDPR NTWVASDGAS AGNSWANGYD IGTRNQDDIL NKIDKEIDST DNFEGFQLLH SVAGGTGSGL GSNLLEALCD RYPKKILTTY SVFPARSSEV VVQSYNTILA LRRLIEDSDA TVVFDNASLL NISGKVFRNP NIDLQHTNQL ISTIISSVTN SIRFPSYMYS SMSSIYSTLI PSPELHFLSP SFTPFTSDYI HDDIAHKGHS SYDVMLDLLD PSNSLVSTAM NNPTYFNVYN TIIGNVEPRQ ISRAMTKLQQ RIKFPSWSSS AMHVNIGRRS PYLPLQPNEN EVSGMMLSNM STVVNVFENA CNTFDKVFAK GAFLNNYNVG DLFQSMQNVQ DEFAESREVV QSLMEDYVAA EQDSYLDDVL VDDENMVGEL EEDLDADGDH KLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays.
  • 基因功能参考文献：
    1. findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1; Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation PMID: 26310304
    2. We propose that SPBs, via the tail of Tub4, promote the assembly of functional +TIP complexes before their deployment to microtubule +ends. PMID: 16899509
    3. The structure of the Saccharomyces cerevisiae gamma-tubulin small complex at 25-A resolution by electron microscopy, is determined. PMID: 17978090
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
  • 蛋白家族：
    Tubulin family
  • 数据库链接：

    KEGG: sce:YLR212C

    STRING: 4932.YLR212C