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  4. Recombinant Saccharomyces cerevisiae Transcriptional activator/repressor MOT3 (MOT3)

Recombinant Saccharomyces cerevisiae Transcriptional activator/repressor MOT3 (MOT3)

  • 货号：
    CSB-YP345009SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP345009SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP345009SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP345009SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP345009SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MOT3
  • Uniprot No.：
  • 别名：
    MOT3; ROX7; YMR070W; YM9916.09; Transcriptional activator/repressor MOT3; Hypoxic gene repressor protein 7; Modulator of transcription protein 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-490
  • 氨基酸序列
    MNADHHLQQQ QQQRQQHQQQ QHQQQQHQHQ HQQQQHTILQ NVSNTNNIGS DSLASQPFNT TTVSSNKDDV MVNSGARELP MPLHQQQYIY PYYQYTSNNS NNNNVTAGNN MSASPIVHNN SNNSNNSNIS ASDYTVANNS TSNNNNNNNN NNNNNNNIHP NQFTAAANMN SNAAAAAYYS FPTANMPIPQ QDQQYMFNPA SYISHYYSAV NSNNNGNNAA NNGSNNSSHS APAPAPGPPH HHHHHSNTHN NLNNGGAVNT NNAPQHHPTI ITDQFQFQLQ QNPSPNLNLN INPAQPLHLP PGWKINTMPQ PRPTTAPNHP PAPVPSSNPV ASNLVPAPSS DHKYIHQCQF CEKSFKRKSW LKRHLLSHSQ QRHFLCPWCL SRQKRKDNLL QHMKLKHTNY LLDELKKNNI IFNYNNSSSS NNNNDNNNNN NSNSASGSGG AGAAAAAATA PENEDGNGYD TNIKTLINDG VLNKDDVKRV LNNLIVSHNK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that affects the expression of a large set of genes. Recognizes and binds to the consensus sequence 5'-[CAT]AGG[TC]A-3' in the promoter region. Plays a major role in the repression of a specific subset of hypoxic genes (e.g. ANB1, DAN1 and HEM13) under aerobic conditions. Acts synergistically with the transcription factor ROX1 to recruit the general repression complex SSN6-TUP1 to the promoter of hypoxic genes. Represses transcription of ergosterol biosynthetic genes. Negatively regulates pheromone-induced gene expression. Can act as a transcriptional activator (e.g. of genes like CYC1, SUC2 and the Ty long terminal repeat delta promoter).
  • 基因功能参考文献：
    1. Both Mot3 and Msn4 bind directly to some promoter regions osmostress-inducible genes. PMID: 23435728
    2. Study reports that a prion formed by the Mot3 transcription factor, [MOT3(+)], governs the acquisition of facultative multicellularity in the budding yeast Saccharomyces cerevisiae. PMID: 23540696
    3. Mot3 and Rox1 are important for growth and modulation of ergosterol levels upon salt stress. Hog1, Mot3, and Rox1 repress ERG gene expression during osmotic stress. PMID: 21299653
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: sce:YMR070W

    STRING: 4932.YMR070W