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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein phosphatase PP1-1 (SIT4)

Recombinant Saccharomyces cerevisiae Serine/threonine-protein phosphatase PP1-1 (SIT4)

  • 中文名称：
    酿酒酵母SIT4重组蛋白
  • 货号：
    CSB-YP324069SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SIT4重组蛋白
  • 货号：
    CSB-EP324069SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SIT4重组蛋白
  • 货号：
    CSB-EP324069SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SIT4重组蛋白
  • 货号：
    CSB-BP324069SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SIT4重组蛋白
  • 货号：
    CSB-MP324069SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SIT4
  • Uniprot No.：
  • 别名：
    SIT4; PPH1; YDL047W; D2693; Serine/threonine-protein phosphatase PP1-1; EC 3.1.3.16
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-311
  • 氨基酸序列
    MVSRGPDEWL ETIKKCQALT ENEMKQLCEM VKELLMEESN IQPVQTPVTV CGDIHGQFHD LLELFRTAGG FPDDINYIFL GDYVDRGYYS LETFTLLMCL KVKYPAKITL VRGNHESRQI TQVYGFYEEC LNKYGSTTVW KYCCQVFDFL TLAAIIDGKI LCVHGGLSPE IRMLDQIRVL SRAQEVPHEG GFSDLLWSDP DNVEAWQVSP RGAGWLFGSK VAREFNHVNG LNLIARAHQL VMEGFKYHFP EKDVVTVWSA PNYCYRCGNV ASVMKVDEDL EPTFKIFSAV PDDYIRESTA NHNNQRAGYF L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.
  • 基因功能参考文献：
    1. suggest that Sit4p-mediated dephosphorylation of Atp2p-T124/T317 downregulates Atp2p alongside with ATP synthase and mitochondrial function PMID: 29719209
    2. Phytoceramide accumulation and Sit4-dependent signaling mediate the mitochondrial dysfunction and shortened lifespan in the yeast model of Niemann-Pick type C1, in part through modulation of the Pkh1-Sch9 pathway. PMID: 28988886
    3. This pathway is functionally relevant to TORC1 signaling since the stress sensitivity of a nonessential TORC1 mutant (tco89Delta) to hydroxyurea and arsenic can be reversed by combining tco89Delta with either hst3Delta, hst4Delta, or sit4Delta Surprisingly, while hst3Delta or hst4Delta rescues the sensitivity tco89Delta has to low concentrations of the TORC1 inhibitor rapamycin, sit4Delta fails to do so PMID: 27343235
    4. The results suggest that the phosphorylated Hxk2 functions downstream of Sit4 in the control of cell cycle, mitochondrial function, oxidative stress resistance and chronological lifespan. PMID: 27163342
    5. Results show that Sit4p and its mammalian orthologue, PP6, regulate traffic from the ER to the Golgi complex which is consistent with its role in coat recycling. PMID: 23864707
    6. Sit4p is essential for rapid translation of HXT1. PMID: 22882630
    7. Data show that the Reg1-Glc7 protein phosphatase 1 and Sit4 type 2A-like phosphatase are largely responsible for dephosphorylation of Thr-210 of Snf1(1-309). PMID: 22589305
    8. Data show that Tec1 protein stability is under control of the nutrient-sensitive target of rapamycin complex 1 (TORC1) signaling pathway via the Tip41-Tap42-Sit4 branch. PMID: 21840851
    9. Epistasis experiments support a model in which Rts3 and Sds23/24 act in parallel to negatively regulate Sit4, while Hse1 confers caffeine resistance via a separate pathway. PMID: 21360731
    10. Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase. PMID: 21464305
    11. These data uncover a new regulatory pathway that connects multidrug resistance to Sit4p function. PMID: 20608983
    12. Sit4 forms a complex with Rrd1 (Ptpa1) that mediates repair of oxidative DNA damage caused by 4-nitroquinoline-1-oxide and Ultraviolet A. PMID: 15150670
    13. Snf1 protein kinase and Sit4 protein phosphatase have opposing functions in regulating TATA-binding protein association with the Saccharomyces cerevisiae INO1 promoter PMID: 15716495
    14. Sit4 activity masked nitrogen source-dependent changes in Gln3-Myc(13) phosphorylation which were clearly visible when SIT4 was deleted. PMID: 17015442
    15. Sit4p regulates the transcriptional repressor activity of Mig1pregulated by a post-transcriptional pathway. PMID: 18022394
    16. sit4-110 has pronounced actin polarization defects under both absence and presence of dipyridyl disulfide. PMID: 18357452
    17. Tap42 functions in the same positive direction as Sit4, Pph21 and Pph22 in Ty1 transcriptional silencing, providing support to the idea that Tap42 may play a positive role in phosphatase activity in response to TORC1 signalling PMID: 18949822
    18. PP2A-dependent Gln3 dephosphorylation parallels conditions where Gln3 is mostly nuclear, while Sit4-dependent and Rap-induced dephosphorylation parallels those where Gln3 is mostly cytoplasmic PMID: 19015262

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    PPP phosphatase family, PP-6 (PP-V) subfamily
  • 数据库链接：

    KEGG: sce:YDL047W

    STRING: 4932.YDL047W