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  4. Recombinant Saccharomyces cerevisiae SEC14 cytosolic factor (SEC14)

Recombinant Saccharomyces cerevisiae SEC14 cytosolic factor (SEC14)

  • 中文名称：
    酿酒酵母SEC14重组蛋白
  • 货号：
    CSB-YP328458SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SEC14重组蛋白
  • 货号：
    CSB-EP328458SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SEC14重组蛋白
  • 货号：
    CSB-EP328458SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SEC14重组蛋白
  • 货号：
    CSB-BP328458SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SEC14重组蛋白
  • 货号：
    CSB-MP328458SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEC14
  • Uniprot No.：
  • 别名：
    SEC14; PIT1; YMR079W; YM9582.04; SEC14 cytosolic factor; Phosphatidylinositol/phosphatidylcholine transfer protein; PI/PC TP
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-304
  • 氨基酸序列
    VTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI ERLDDSTLLR FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK PLIAKFYPQY YHKTDKDGRP VYFEELGAVN LHEMNKVTSE ERMLKNLVWE YESVVQYRLP ACSRAAGHLV ETSCTIMDLK GISISSAYSV MSYVREASYI SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI FILGSSYQKE LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA FSMK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes in vitro. Essential for viability and secretion. Exchanges its bound phospholipid with phospholipid monomers that reside in membrane bilayers. Regulates specific trans-Golgi export pathways, like transport from endosomes to the trans-Golgi or transport from the plasma membrane to the vacuole at the level of the endosome. Increased membrane curvature and lipid unsaturation levels at the trans-Golgi membrane promotes membrane binding and phospholipid transfer of SEC14. Thus, SEC14 may act at the trans-Golgi membrane to exchange lipids and promote vesicle formation. The phosphatidylcholine-bound form of SEC14 represses the CDP-choline pathway activity by inhibiting CCTase, the rate-determining enzyme of the CDP-choline pathway. PI binding/transfer is dispensable for function in vivo. Required for trafficking and localization of lipid raft proteins to the plasma membrane.
  • 基因功能参考文献：
    1. Data suggest that SEC14L and SFH1 undergo conformational changes as phosphatidylinositol/phosphatidylcholine bind to hydrophobic pockets/ligand binding sites. [review-like article] PMID: 25233419
    2. Sec14 and Sec14 like proteins form central conduits to integrate multiple aspects of lipid metabolism with productive phosphoinositide signaling. (Review) PMID: 22374094
    3. The crystal structure of Sec14 family homologue (Sfh)3 is the first report of a dimeric Sec14 protein and provides clues for its functional study. PMID: 23519406
    4. Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14. PMID: 23383173
    5. To launch a detailed structural and functional characterization of Sfh3, the recombinant protein was purified to homogeneity, diffraction-quality crystals were produced and a native X-ray data set was collected to 2.2 A resolution PMID: 22102037
    6. Sec14 protein is a peripheral Golgi membrane protein essential for Golgi secretory function and cell viability[review]. PMID: 16118014
    7. This review describes Sec14p, the major (phosphatidylinositol)/PC (phosphatidylcholine)-transfer protein in yeast, which co-ordinates lipid metabolism with protein transport from the Golgi complex. PMID: 16709158
    8. The application of EPR spectrscopy to investigate the chemical properties of binding between phosphatidylcholine and Sec14 is reported. PMID: 16997918
    9. molecular dynamics suggests large rigid body motions within Sec14p accompany closing and opening of an A(10)/T(4)/A(11) helical gate, and that "state-of-closure" of this helical gate determines access to the Sec14p phospholipid binding cavity PMID: 17344474
    10. This review focuses on five yeast proteins that share significant sequence homology with Sec14p and have distinct subcellular localizations with functions related to lipid metabolism, phosphoinositide mediated signaling and membrane trafficking. PMID: 17395532
    11. a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo PMID: 18243114

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  • 亚细胞定位：
    Golgi apparatus membrane; Peripheral membrane protein. Cytoplasm.
  • 数据库链接：

    KEGG: sce:YMR079W

    STRING: 4932.YMR079W