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  4. Recombinant Saccharomyces cerevisiae Ribosome biogenesis protein NOP53 (NOP53)

Recombinant Saccharomyces cerevisiae Ribosome biogenesis protein NOP53 (NOP53)

  • 中文名称：
    酿酒酵母NOP53重组蛋白
  • 货号：
    CSB-YP612279SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母NOP53重组蛋白
  • 货号：
    CSB-EP612279SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母NOP53重组蛋白
  • 货号：
    CSB-EP612279SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母NOP53重组蛋白
  • 货号：
    CSB-BP612279SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母NOP53重组蛋白
  • 货号：
    CSB-MP612279SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NOP53
  • Uniprot No.：
  • 别名：
    NOP53; YPL146C; LPI2C; P2610; Ribosome biogenesis protein NOP53; Nucleolar protein 53
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-455
  • 氨基酸序列
    MAPTNLTKKP SQYKQSSRKG KKAWRKNIDL SDVEQYMEKK IDHEITHGTS DITSLQNDAL FHVDVEGDEI LKNKLIKRKQ IKKVLKSKEI LDAVKTNSKI AALNHHKNSS GNPNKIQGVS KHELKKLMAL AGRVHGESKI KNRVAKDGLV KTTAGDLWGE ESNSKKQKVK LPSGIKLDVE KKDQIPEELL KKSTTSWSTA SVRPSTLDIE PIAVKEFTEI PHAGKSYNPN NKAWSELINK EYKEEKARED ERIALEKYKE RIRHLMETLD DNEEEESSSN EEEEEEEEEN ENENESTQCS GSDKEIKLSI NKPVKNKKKT KYQRNKAKRH EEKVKLQQEL KELRQRVKDL EEVINSEETE ILSAIESDSN KVKKSKKNKK HKLGTKYSVI DERLEIKFSD ELSDSLRKLK PEGNLLYDTV RKLQSSGKVE TRVPVRKGRK YKQKITEKWT HKDFK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Late-acting factor in the nuclear maturation of 60S ribosomal subunits, which is required for normal acquisition of export competence. Required for the export of the large subunit. Acts to stimulate the RNase activity of the exosome complex, and may recruit the exosome to 7S pre-rRNA for processing. Associates with numerous RNAs including the 27S and 7S pre-rRNAs and the box H/ACA snoRNA snR37. Also interacts (via N-terminal region) with the mature 25S rRNA and the mature 5.8S rRNA.
  • 基因功能参考文献：
    1. Deletion or mutations within NOP53 cause significant growth defects and display significant 60 S subunit deficiencies, an imbalance in the 40 S:60 S ratio, and defects in progression beyond the 27 S stage of 25 S rRNA maturation during 60 S biogenesis. PMID: 15686447
    2. Nop53p is a late-acting factor in the nuclear maturation of 60S ribosomal subunits, which is required for normal acquisition of export competence. PMID: 16043506
    3. protein A-tagged Nop53p coprecipitated the 27S and 7S pre-rRNAs, and His-Nop53p also bound directly 5.8S rRNA in vitro, which is consistent with a role for Nop53p in pre-rRNA processing PMID: 16128814
  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族：
    NOP53 family
  • 数据库链接：

    KEGG: sce:YPL146C

    STRING: 4932.YPL146C