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  4. Recombinant Saccharomyces cerevisiae Protein ESC2 (ESC2)

Recombinant Saccharomyces cerevisiae Protein ESC2 (ESC2)

  • 货号：
    CSB-YP312353SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP312353SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP312353SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP312353SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP312353SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ESC2
  • Uniprot No.：
  • 别名：
    ESC2; YDR363W; Protein ESC2; Establishes silent chromatin protein 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-456
  • 氨基酸序列
    MTGDSRSISE PSINLDPDNT SFSDENSDDF FMDNSYDIDE IDHSDESNRQ SVIVDSKVTV PPSKHSTLTL SDSEDSDAKE QHQSLSRSSS KNVNIEDITE PKPDKPSGRT RGRSVMKESV VEINSSESDL DEDKNFPRSR SRSRSSIRSI SPAGKYKRQK SSLLYTYDEN DDFFKELAKE AKKSTTISKE STPDQRKRVY NIKFLSKLEG TINKAVQVKV LGKYEFSKIL PAALDGLMKS YKIPKVMKDI YKVENVTLYW NNAKLLTFMT CNSLHIPQDF ENEVSDIDVT IVSKEYEKNF EATLESKLKE EEAALLIKER QEMERKLEKK RNEQEESEYR EFESELKNVE ETQEIKENDT VMNTKLLQEG GSLSGNSSSM EEVMRIALMG QDNKKIYVHV RRSTPFSKIA EYYRIQKQLP QKTRVKLLFD HDELDMNECI ADQDMEDEDM VDVIID
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be a substrate targeting component of a cullin-RING-based E3 ubiquitin-protein ligase complex RTT101(MMS1-ESC2). Involved in HMR and telomere silencing via the recruitment or stabilizing of the SIR (silent information regulators) complex.
  • 基因功能参考文献：
    1. The data point to the possibility that the involvement of Esc2 in the metabolism of sister chromatid junctions is, in part, via modulation of the activity of the Mus81 complex. PMID: 27694623
    2. Tel1, Mre11 and Esc2 can silence a reporter gene dependent on the Sir, as well as on other repair proteins. Furthermore, these proteins aid in the localization of silenced domains to specific compartments in the nucleus. PMID: 25631822
    3. Esc2 binds stalled replication forks and counteracts the anti-recombinase Srs2 helicase via a two-faceted mechanism involving chromatin recruitment and turnover of Srs2. PMID: 26443850
    4. Esc2 is functionally equivalent to a stable SUMO fusion protein and plays important roles in facilitating DNA replication fork progression and sister chromatid cohesion PMID: 18757937
    5. In the absence of both Esc2 and Sgs1, the intra-S-phase DNA damage checkpoint response is compromised after exposure to MMS PMID: 19158388

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: sce:YDR363W

    STRING: 4932.YDR363W