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  4. Recombinant Saccharomyces cerevisiae Protein BMH2 (BMH2)

Recombinant Saccharomyces cerevisiae Protein BMH2 (BMH2)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Protein BMH2(BMH2),Yeast
  • 货号：
    CSB-YP333932SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Protein BMH2(BMH2),Yeast
  • 货号：
    CSB-EP333932SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Protein BMH2(BMH2),Yeast
  • 货号：
    CSB-EP333932SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Protein BMH2(BMH2),Yeast
  • 货号：
    CSB-BP333932SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Protein BMH2(BMH2),Yeast
  • 货号：
    CSB-MP333932SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BMH2
  • Uniprot No.：
  • 别名：
    BMH2; YDR099W; YD8557.08; Protein BMH2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-273
  • 氨基酸序列
    SQTREDSVY LAKLAEQAER YEEMVENMKA VASSGQELSV EERNLLSVAY KNVIGARRAS WRIVSSIEQK EESKEKSEHQ VELIRSYRSK IETELTKISD DILSVLDSHL IPSATTGESK VFYYKMKGDY HRYLAEFSSG DAREKATNSS LEAYKTASEI ATTELPPTHP IRLGLALNFS VFYYEIQNSP DKACHLAKQA FDDAIAELDT LSEESYKDST LIMQLLRDNL TLWTSDISES GQEDQQQQQQ QQQQQQQQQQ QAPAEQTQGE PTK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84 PMID: 28877665
    2. Both Bmh1 and Bmh2 associate with the promoters of at least some of these genes. BMH2, but not BMH1, attenuates the repression of genes involved in some functions required for ribosome biogenesis. BMH2 also attenuates the activation of genes sensitive to nitrogen catabolite repression. PMID: 27919033
    3. Bmh2 is required for nuclear Gln3 localization and appears to function downstream of Ure2. PMID: 28007891
    4. demonstrated the interaction of Rod1 with Hxt6 and showed that the localization of the Rod1-Hxt6 complex at the plasma membrane is affected by carbon source and is reduced upon overexpression of SNF1 and BMH2 PMID: 27261460
    5. Data indicate that transcription activation is regulated negatively by two redundant 14-3-3 isoforms, Bmh1 and Bmh2. PMID: 25355315
    6. Bmh1 and Bmh2 inhibit transcription by binding to Adr1 at promoters that contain a preinitiation complex, demonstrating that Bmh-mediated inhibition is not due to nuclear exclusion, inhibition of DNA binding, or RNA polymerase II (Pol II) recruitment. PMID: 23207903
    7. Yeast BMH1 and BMH2 possess a distinctly variant C-terminal compared to human 14-3-3 zeta protein. PMID: 20384366
    8. BMH2 function as essential factors for the assembly and maintenance of the pre-replication complex during G1 phase. PMID: 19934224
    9. genome-wide transcription analysis of a temperature-sensitive bmh2 mutant PMID: 15142031
    10. high-dosage suppressors of the growth defects of temperature-sensitive bmh1 and bmh2 mutants PMID: 16648583
    11. role of 14-3-3 in the trafficking of this multimeric Pmp2p reporter is isoform-specific PMID: 16734667
    12. Acm1 and Bmh1/Bmh2 constitute a specialized inhibitor of anaphase-promoting complex/CCdh1. PMID: 17030612
    13. The 14-3-3 functional interaction appears to modulate Rad53 activity, reminiscent of 14-3-3's effect on human Raf1 kinase and distinct from the indirect mode of regulation by 14-3-3 observed for Chk1 or Cdc25. PMID: 17299042
    14. We propose that Bmh1 and -2 are common regulators of many cell signaling modules and pathways mediated by protein phosphorylation and regulate a variety of biological events by coordinately controlling the identified multiplex phosphoprotein components. PMID: 17559233
    15. Nucleocytoplasmic shuttling of Pik1p involves phosphorylation and 14-3-3 proteins bind Pik1p in the cytoplasm. PMID: 18172025

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    14-3-3 family
  • 数据库链接：

    KEGG: sce:YDR099W

    STRING: 4932.YDR099W