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  4. Recombinant Saccharomyces cerevisiae NEDD8-activating enzyme E1 regulatory subunit (ULA1)

Recombinant Saccharomyces cerevisiae NEDD8-activating enzyme E1 regulatory subunit (ULA1)

  • 中文名称：
    酿酒酵母ULA1重组蛋白
  • 货号：
    CSB-YP609121SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母ULA1重组蛋白
  • 货号：
    CSB-EP609121SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母ULA1重组蛋白
  • 货号：
    CSB-EP609121SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母ULA1重组蛋白
  • 货号：
    CSB-BP609121SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母ULA1重组蛋白
  • 货号：
    CSB-MP609121SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ULA1
  • Uniprot No.：
  • 别名：
    ULA1; ENR2; YPL003W; LPA14W; NEDD8-activating enzyme E1 regulatory subunit; E1 N-terminus-related protein 2; Ubiquitin-activating enzyme E1-like 1; Ubiquitin-like activation protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-462
  • 氨基酸序列
    MERYDRQLRL WGALGQDSLN RSRVCVVGPA TPLLQEVFKN LVLAGISSLT WLKVECAVQS GSLFLAELKK DLEPLASKQL EYEENDLRKT LQQPQYDWTR FSVVILTCIG EQTAMLDLNE IRRQRGTKFP PVLNTFVSGF YGYIYLVLSE THFVLQAHPD SKKYDLRLQN PWPELINYVD TFDLSKMDTA TFSGIPYTVL LMKCIAKLER DGNNGRITID QMKKVLDQIC LPLGNDVIYE PNYVEAKRYA YLACSQNDCC KELEDLLRNL EISDYGNDWH DTYNYEILTL LLTLKNIAKE NGELSFQPLT GTLPDMESTT ENYIRLKKLY EVKAKLDKSR VEESLARSKK IVSQDVLETF CSHYGEVRKI LPPKSDLLGI FSTSNALLDA LVMVQFWEQP AVTAEDKDEF IGLRVDDNYS VMAFFGGAVV QEAIKLITHH YVPIDNLFLY NGINNSSATY KI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the dimeric UBA3-ULA1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBC12.
  • 蛋白家族：
    Ubiquitin-activating E1 family, ULA1 subfamily
  • 数据库链接：

    KEGG: sce:YPL003W

    STRING: 4932.YPL003W