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  4. Recombinant Saccharomyces cerevisiae NAD-dependent histone deacetylase HST4 (HST4)

Recombinant Saccharomyces cerevisiae NAD-dependent histone deacetylase HST4 (HST4)

  • 货号：
    CSB-YP345310SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP345310SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP345310SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP345310SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP345310SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HST4
  • Uniprot No.：
  • 别名：
    HST4; YDR191W; YD9346.03NAD-dependent histone deacetylase HST4; EC 2.3.1.286; Homologous to SIR2 protein 4; Regulatory protein SIR2 homolog 4
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-370
  • 氨基酸序列
    MKQKFVLPIT PPSTAEKKPQ TENRCNENLK PRRLLPQLKK SVRNRKPRLS YRPELNSVFD LDAYVDSTHL SKSQRHHMDR DAGFISYALN YSKRMVVVSG AGISVAAGIP DFRSSEGIFS TVNGGSGKDL FDYNRVYGDE SMSLKFNQLM VSLFRLSKNC QPTKFHEMLN EFARDGRLLR LYTQNIDGLD TQLPHLSTNV PLAKPIPSTV QLHGSIKHME CNKCLNIKPF DPELFKCDDK FDSRTEIIPS CPQCEEYETV RKMAGLRSTG VGKLRPRVIL YNEVHPEGDF IGEIANNDLK KRIDCLIIVG TSLKIPGVKN ICRQFAAKVH ANRGIVLYLN TSMPPKNVLD SLKFVDLVVL GDCQHVTSLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NAD-dependent histone deacetylase, which contributes together with HST3 to histone H3 'Lys-56' deacetylation, regulation of telomeric silencing, proper cell cycle progression, DNA damage control, DNA recombination, and genomic maintenance.
  • 基因功能参考文献：
    1. Hst4p plays a pivotal role in biotin metabolism and cellular energy homeostasis, and data supports that Hst4p is a functional yeast homologue of the sirtuin deacetylase SIRT3. PMID: 26158509
    2. Our results suggest that Hst3 and Hst4 regulate replicative lifespan through their ability to deacetylate histone H3-K56 to minimize genomic instability. PMID: 21401809
    3. That acetylation of Histone H3 by Hst4 takes place during checkpoint regulation was determined. PMID: 16818235
    4. Specific functions were found for HST4. PMID: 17446861
    5. cells lacking Hst4p, in which H3 K56 is constitutively hyperacetylated, exhibit hallmarks of spontaneous DNA damage PMID: 18579506
    6. HST3 HST4 play critical roles in maintaining the hypoacetylated state of lysine56 on histone H3 within silent chromatin. PMID: 18799617

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Sirtuin family, Class I subfamily
  • 数据库链接：

    KEGG: sce:YDR191W

    STRING: 4932.YDR191W