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  4. Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RAD52 (RAD52)

Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RAD52 (RAD52)

  • 中文名称：
    酿酒酵母RAD52重组蛋白
  • 货号：
    CSB-YP361943SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RAD52重组蛋白
  • 货号：
    CSB-EP361943SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RAD52重组蛋白
  • 货号：
    CSB-BP361943SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RAD52重组蛋白
  • 货号：
    CSB-MP361943SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD52
  • Uniprot No.：
  • 别名：
    RAD52; YML032C; DNA repair and recombination protein RAD52
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-471
  • 氨基酸序列
    MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ RSTRREVGRP KINPLHARKP T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • 基因功能参考文献：
    1. Global chromosome mobility is regulated by the Rad51 recombinase and its mediator, Rad52. PMID: 30181361
    2. Rad52 inverse strand exchange plays an important role in RNA-templated double strand break repair in vivo. PMID: 28602639
    3. The C-terminal region of yRad52, but not of hRAD52, is involved in ssDNA annealing. This suggests that the second DNA binding site is required for the efficient ssDNA annealing by yRad52. We propose an updated model of Rad52-mediated ssDNA annealing. PMID: 27362509
    4. Rad52 is needed to repair telomere attrition-induced replication stress PMID: 27428329
    5. investigated the contribution of the nuclear double-strand break repair (DSBR) proteins Rad51p, Rad52p and Rad59p in mtDNA repair PMID: 26540255
    6. the homeologous pairing between the terminal TG1-3 repeats at VII-L and internal repeats on other chromosome ends was largely independent of Rad51, but instead it was facilitated by Rad59 that stimulates Rad52 strand annealing activity PMID: 25375789
    7. It was found that, despite the absence of self-homology on the T-DNA, the homologous repair proteins Rad52 and Rad51 are involved in T-DNA circle formation. PMID: 24460832
    8. The study suggested that phenylalanine-349 and tyrosine-409 are key residues in the C-terminal half of Rad52 and probably play an important role in the recombination mediator activity. PMID: 24163251
    9. This conclusion is strengthened by the finding that Rad52 is often associated with complete Rad51 filaments in vitro PMID: 24130504
    10. The Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. PMID: 23790361
    11. New scenario for homologous recombination where Rad52 and Rad51 are recruited to the fork to promote DNA damage tolerance by distinct and cell cycle-regulated replicative and repair functions. PMID: 23563117
    12. Our results describe 8 new genes involved in SCR, including functions such as histone acetylation/deacetylation, SUMO-Ubiquitin metabolism, and stress response, as well as an allele of RAD52 PMID: 23357952
    13. Ty1 sequence-specific gross chromosomal rearrangements are RAD52-dependent. PMID: 21637792
    14. Rad52 within the complex associates with double-stranded DNA to assist Rad51-mediated homologous pairing. PMID: 21454474
    15. results highlight the importance of Rad52 SUMOylation as part of a 'quality control' mechanism regulating the efficiency of recombination and DNA repair. PMID: 20371517
    16. result suggests that spontaneous DNA lesions that require recombinational repair occur at the same frequency in wild-type and rad52-Y66A cells, but that the recombination process is slow in rad52-Y66A cells. PMID: 19892607
    17. Results from chromatin immunoprecipitation experiments find that rad52-R70A associates with DNA double-strand breaks and promotes recruitment of Rad51 as efficiently as wild-type Rad52. PMID: 19812039
    18. Increases in transposition activity in sir4 mutant strains at high temperature is dependent on the RAD52 gene. PMID: 15454529
    19. These results imply that telomeres can use looped-out telomeric rings to promote telomere-telomere recombination through Rad52-, Rad50-, and polymerase delta in telomerase-deficient Saccharomyces cerevisiae. PMID: 15701795
    20. examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties PMID: 16565518
    21. Multiple Rad52 protein species are due to promiscuous choice of start codons as well as post-translational modification. PMID: 16707661
    22. The rearrangement frequency was found to increase with array size, and partial complementation of the rad27Delta mutation by hFEN1 demonstrated that the production of novel CEB1 alleles is Rad52 and Rad51 dependent. PMID: 16914748
    23. S. cerevisiae Rad52 protein and its eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break. PMID: 17040915
    24. Error-free RAD52 pathwaydetermines spontaneous mutagenesis in Saccharomyces cerevisiae PMID: 17396018
    25. The contributions of the RAD51, RAD52, and RAD54 genes and of the RAD50 and XRS2 genes to the postreplicational repair of ultraviolet rays-damaged DNA, is examined. PMID: 17785441
    26. These results provide a mechanistic framework for rationalizing the multi-faceted role of Rad52 in recombination and DNA repair. PMID: 18310075
    27. Results show that Rad52 functions in temporally and biochemically distinct reactions and suggest a general annealing mechanism for reuniting DSB ends during recombination. PMID: 18313389
    28. regulation of Rad52-mediated annealing suggests a control function for Rad51 in deciding the recombination path taken for a processed DNA break; the ssDNA can be directed to either Rad51-mediated DNA strand invasion or to Rad52-mediated DNA annealing PMID: 18337252
    29. These findings indicate that nuclear localization of Rad52 is pre-requisite for its sumoylation. PMID: 18482582
    30. support a role for Rad52-promoted annealing in the formation of Holliday junctions in double-stranded break repair PMID: 19204284
    31. Rad52 interaction with replication protein-A (RPA) requires multiple molecules of RPA to be associated with ssDNA, suggesting that multiple contacts between the Rad52 ring and RPA-ssDNA filament are needed for stable binding. PMID: 19445949
    32. condensin and Rad52 have opposing roles in the control of rDNA stability under nutrient starvation conditions PMID: 19520859
    33. The structure and functions of Rad52 are briefly reviewed. The DNA annealing & mediator functions of Rad52 play a central role in homologous recombination-based DNA double-strand-break repair. Review. PMID: 19706272

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD52 family
  • 数据库链接：

    KEGG: sce:YML032C

    STRING: 4932.YML032C