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  4. Recombinant Saccharomyces cerevisiae DNA polymerase delta small subunit (POL31)

Recombinant Saccharomyces cerevisiae DNA polymerase delta small subunit (POL31)

  • 中文名称：
    酿酒酵母POL31重组蛋白
  • 货号：
    CSB-YP341656SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母POL31重组蛋白
  • 货号：
    CSB-EP341656SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母POL31重组蛋白
  • 货号：
    CSB-EP341656SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母POL31重组蛋白
  • 货号：
    CSB-BP341656SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母POL31重组蛋白
  • 货号：
    CSB-MP341656SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    POL31
  • Uniprot No.：
  • 别名：
    POL31; HUS2; HYS2; SDP5; YJR006W; J1427; YJR83.7DNA polymerase delta small subunit; EC 2.7.7.7; Hydroxyurea-sensitive protein 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-487
  • 氨基酸序列
    MDALLTKFNE DRSLQDENLS QPRTRVRIVD DNLYNKSNPF QLCYKKRDYG SQYYHIYQYR LKTFRERVLK ECDKRWDAGF TLNGQLVLKK DKVLDIQGNQ PCWCVGSIYC EMKYKPNVLD EVINDTYGAP DLTKSYTDKE GGSDEIMLED ESGRVLLVGD FIRSTPFITG VVVGILGMEA EAGTFQVLDI CYPTPLPQNP FPAPIATCPT RGKIALVSGL NLNNTSPDRL LRLEILREFL MGRINNKIDD ISLIGRLLIC GNSVDFDIKS VNKDELMISL TEFSKFLHNI LPSISVDIMP GTNDPSDKSL PQQPFHKSLF DKSLESYFNG SNKEILNLVT NPYEFSYNGV DVLAVSGKNI NDICKYVIPS NDNGESENKV EEGESNDFKD DIEHRLDLME CTMKWQNIAP TAPDTLWCYP YTDKDPFVLD KWPHVYIVAN QPYFGTRVVE IGGKNIKIIS VPEFSSTGMI ILLDLETLEA ETVKIDI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
  • 基因功能参考文献：
    1. Pol31 and Pol32 subunits of yeast DNA polymerase delta are also essential subunits of DNA polymerase zeta. PMID: 22711820
    2. Loss of the last four amino acids at the C-terminal end of Pol3 impairs the interaction with Pol31. PMID: 20813592
    3. study reveals that Pol31 engages in crosstalk with the other Poldelta subunits, as well as with Mgs1, Rad18 and PCNA; some of the Pol31 regions that are important for such functional cooperations are identified PMID: 16949354
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase delta/II small subunit family
  • 数据库链接：

    KEGG: sce:YJR006W

    STRING: 4932.YJR006W