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  4. Recombinant Saccharomyces cerevisiae DNA endonuclease SAE2 (SAE2)

Recombinant Saccharomyces cerevisiae DNA endonuclease SAE2 (SAE2)

  • 中文名称：
    酿酒酵母SAE2重组蛋白
  • 货号：
    CSB-YP342841SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SAE2重组蛋白
  • 货号：
    CSB-EP342841SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SAE2重组蛋白
  • 货号：
    CSB-EP342841SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SAE2重组蛋白
  • 货号：
    CSB-BP342841SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SAE2重组蛋白
  • 货号：
    CSB-MP342841SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SAE2
  • Uniprot No.：
  • 别名：
    SAE2; COM1; YGL175C; G1639DNA endonuclease SAE2; EC 3.1.-.-; Completion of meiotic recombination protein 1; Sporulation in the absence of SPO11 protein 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MVTGEENVYL KSSLSILKEL SLDELLNVQY DVTTLIAKRV QALQNRNKCV LEEPNSKLAE ILCHEKNAPQ QSSQTSAGPG EQDSEDFILT QFDEDIKKES AEVHYRNENK HTVQLPLVTM PPNRHKRKIS EFSSPLNGLN NLSDLEDCSD TVIHEKDNDK ENKTRKLLGI ELENPESTSP NLYKNVKDNF LFDFNTNPLT KRAWILEDFR PNEDIAPVKR GRRKLERFYA QVGKPEDSKH RSLSVVIESQ NSDYEFAFDN LRNRSKSPPG FGRLDFPSTQ EGNEDKKKSQ EIIRRKTKYR FLMASNNKIP PYEREYVFKR EQLNQIVDDG CFFWSDKLLQ IYARC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endonuclease that cooperates with the MRX complex in processing meiotic and mitotic double-strand breaks by allowing the endonucleolytic removal of SPO11 from the break sites and ensuring both resection and intrachromosomal association of the broken ends. Required for proper recovery from checkpoint-mediated cell cycle arrest after DNA damage. MRX complex and SAE2 remove a small oligonucleotide(s) from the DNA ends to form an early intermediate which is rapidly processed by EXO1 and/or SGS1 to generate extensive tracts of single-stranded DNA that serve as substrate for RAD51. Plays a transitional role in the dissociation of MRE11 from, and the recruitment of RAD52 to, repair foci. Ensures that both ends of a DSB participate in a recombination event and impairs the formation of palindromic structures in the genome. With TEL1, promotes microhomology-mediated end joining (MMEJ) but inhibits non-homologous end joining (NHEJ), likely by regulating MRE11-dependent ssDNA accumulation at DNA break. SAE2 and MRX are particularly important for removal of hairpins, bulky adducts and other irregular end structures. Facilitates telomere length reequilibration and subsequent checkpoint switch off. Involved in homing efficiency of VMA1 intein VDE and in repair of transposon excision sites.
  • 基因功能参考文献：
    1. MRX-Sae2 efficiently cleaves near Ku-blocked DNA ends, which shows how recombination competes with NHEJ in the S/G2 phase when Sae2 is phosphorylated and thus capable of promoting endonucleolytic DNA cleavage by MRX PMID: 29321179
    2. s find that the ability of Sae2 to promote MRX nuclease functions is important for DNA damage survival, particularly in the absence of Dna2 nuclease activity. PMID: 28970327
    3. CtIP/Ctp1/Sae2/Com1 role in removal of DNA double strand breaks through DSB repair by homologous recombination is reviewed. PMID: 28623092
    4. our data suggest a direct role for Sae2 nuclease activity in processing of the DNA structures that arise during replication and DNA damage and provide insights into the mechanism underlying Mre11-Sae2-mediated abrogation of replication stressrelated defects in S. cerevisiae. PMID: 27966484
    5. DNA end resection by Sae2 and Sgs1 is dispensable for normal telomere maintenance by telomerase. PMID: 25254351
    6. without Sae2 or Mre11 nuclease activity, Mre11 bound to partly processed DSBs impairs strand invasion and HR. PMID: 25899817
    7. threonine-specific phosphorylation of Sae2 by Mec1 and Tel1 contributes to DNA repair and genome maintenance via its interactions with Rad53 and Dun1 PMID: 25762720
    8. We found that Sae2 sumoylation occurs independently of its phosphorylation, and the two modifications act in synergy to increase soluble forms of Sae2. PMID: 25569253
    9. DNA damage also triggers removal of Sae2 through autophagy. PMID: 24344201
    10. we provide direct molecular characterization of coincident resection at random, radiation-induced DSBs and show that rapid and coincident initiation of resection at DSBs requires MRX, Sae2 protein, and Mre11 nuclease PMID: 23555316
    11. Lif1 phosphorylation at Ser261 is probably involved in micro-homology-dependent end joining associated with producing single-stranded DSB ends that are formed by Sae2 as early intermediates in the homologous recombination pathway. PMID: 22563681
    12. Data show that Mre11p and Sae2p are not required for DSB formation at FS2 or the subsequent repair of these DSBs. PMID: 19635935
    13. participates in DSB single strand annealing repair by ensuring both resection and intrachromosomal association of the broken ends PMID: 16162495
    14. checkpoint-mediated phosphorylation of Sae2 is important to support its meiotic recombination functions PMID: 16861895
    15. Results idientify COM1, a novel plant gene essential for meiosis that is related to the human CtIP and the yeast COM1/SAE2 gene. PMID: 18007598
    16. Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions. PMID: 18245357
    17. Phosphorylation of the three C-terminal phosphorylation sites of Sae2 is necessary for DNA double-strand break end resection. PMID: 18670132
    18. cell-cycle control of double strand break resection in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein PMID: 18716619
    19. When Sae2 is absent, in addition to Exo1 and Sgs1, unprocessed DSBs accumulate and homology-dependent repair fails PMID: 18806779
    20. Data show tha Sae2 and the Sgs1 RecQ helicase control two distinct but partially complementary pathways for nucleolytic processing telomeres, with Sae2 function requiring its serine 267 phosphorylation. PMID: 19595717

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Accumulates in foci at the precise time when MRE11 foci disassemble and RAD52 foci assemble (PubMed:18670132). Remains associated with DSBs along with MRE11 in nuclease-deficient cells (PubMed:18670132).
  • 蛋白家族：
    COM1/SAE2/CtIP family
  • 数据库链接：

    KEGG: sce:YGL175C

    STRING: 4932.YGL175C