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  4. Recombinant Saccharomyces cerevisiae Cytosolic Fe-S cluster assembly factor NBP35 (NBP35)

Recombinant Saccharomyces cerevisiae Cytosolic Fe-S cluster assembly factor NBP35 (NBP35)

  • 货号：
    CSB-YP344831SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP344831SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP344831SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP344831SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP344831SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NBP35
  • Uniprot No.：
  • 别名：
    NBP35; YGL091CCytosolic Fe-S cluster assembly factor NBP35; Nucleotide-binding protein 35
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MTEILPHVND EVLPAEYELN QPEPEHCPGP ESDMAGKSDA CGGCANKEIC ESLPKGPDPD IPLITDNLSG IEHKILVLSG KGGVGKSTFA AMLSWALSAD EDLQVGAMDL DICGPSLPHM LGCIKETVHE SNSGWTPVYV TDNLATMSIQ YMLPEDDSAI IWRGSKKNLL IKKFLKDVDW DKLDYLVIDT PPGTSDEHIS INKYMRESGI DGALVVTTPQ EVALLDVRKE IDFCKKAGIN ILGLVENMSG FVCPNCKGES QIFKATTGGG EALCKELGIK FLGSVPLDPR IGKSCDMGES FLDNYPDSPA SSAVLNVVEA LRDAVGDV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NBP35-CFD1 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Required for biogenesis and export of both ribosomal subunits, which may reflect a role in assembly of the Fe/S clusters in RLI1, a protein which performs rRNA processing and ribosome export.
  • 基因功能参考文献：
    1. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase. PMID: 26195633
    2. a model in which Cfd1-Nbp35 interaction increases the lability of assembled FeS on the Nbp35 scaffold for transfer to target apo-FeS proteins. PMID: 23798678
    3. a model for the mode of Fe-S cluster coordination within the Cfd1-Nbp35 complex, explaining its specific properties as a Fe-S scaffold device. PMID: 22362766
    4. Nbp35 NTPases function in Fe/S protein maturation PMID: 15728363
    5. suggest that the Cfd1-Nbp35 complex functions as a novel scaffold for [Fe-S] cluster assembly in the eukaryotic cytosol PMID: 17401378

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Mrp/NBP35 ATP-binding proteins family, NUBP1/NBP35 subfamily
  • 数据库链接：

    KEGG: sce:YGL091C

    STRING: 4932.YGL091C