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  4. Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1 (CDC28)

Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1 (CDC28)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1(CDC28),Yeast
  • 货号：
    CSB-YP365446SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1(CDC28),Yeast
  • 货号：
    CSB-EP365446SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1(CDC28),Yeast
  • 货号：
    CSB-EP365446SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1(CDC28),Yeast
  • 货号：
    CSB-BP365446SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Cyclin-dependent kinase 1(CDC28),Yeast
  • 货号：
    CSB-MP365446SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDC28
  • Uniprot No.：
  • 别名：
    CDC28; CDK1; HSL5; SRM5; YBR160W; YBR1211Cyclin-dependent kinase 1; CDK1; EC 2.7.11.22; Cell division control protein 28; Cell division protein kinase 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-298
  • 氨基酸序列
    SGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is essential for the completion of the start, the controlling event, in the cell cycle. More than 200 substrates have been identified.
  • 基因功能参考文献：
    1. Esp1 is phosphorylated by Cdk1.Cdk1 role in the regulation pericentric cohesin and anaphase onset. PMID: 29561844
    2. the G1/S transition, Cdk1 phosphorylates and activates the enzyme Nth1, which funnels the storage carbohydrate trehalose into central carbon metabolism. Trehalose utilization fuels anabolic processes required to reliably complete cell division. PMID: 27203178
    3. Co-regulation of cell division processes and metabolic processes by CDK activity may be a general phenomenon important for co-ordinating the cell cycle with growth in S.cerevisiae. PMID: 27203179
    4. Study reports a novel Cdk1-interacting protein, YPL014W, named Cip1 (for Cdk1-interacting protein 1); results show that Cip1 specifically interacts with G1 /S-phase Cln2-Cdk1 complex but not with S-phase Clb5-Cdk1 or M-phase Clb2-Cdk1 complexes. PMID: 27005485
    5. Phosphorylation of the MRX complex after DNA damage and during mitosis are regulated independently of each other by Tel1/ATM and Cdc28/Cdk1 kinases. PMID: 24553123
    6. We propose that the DNA damage checkpoint stabilizes Ndd1 by inhibiting Cdk1, which we show is required for targeting Ndd1 for destruction. PMID: 25894965
    7. A direct role of CDC28 is in the phosphorylation of the Cac1p subunit of CAF-I and its loading to chromatin and the regulation of chromatin assembly during DNA replication. PMID: 25602519
    8. the unique sensitivity of the chromosome condensation machinery for the kinase activity of Cdk1 acts as a major driving force for the compaction of chromatin at mitotic entry PMID: 25691469
    9. Results suggest that serine-2 CTD phosphorylation in CTDK-I is required for Rtr1 recruitment to RNAPII during transcription elongation. PMID: 24671508
    10. The Cdk1-dependent phosphorylation state of Iqg1 is a determining factor in the timing of bud neck localization of both Iqg1 and actin. PMID: 24413167
    11. Coordinated regulation of the transcription factor network by Cdk1 is necessary for faithful cell division. PMID: 24714560
    12. Cdc28 CDK1 is controlled by a stress-induced long noncoding RNA PMID: 24508389
    13. Transition from meiotic prophase to mitosis requires modulation of Cdk1 activity to coordinate cell-cycle events. Swe1 prevents formation of multinucleate cells by restraining M phase CDK activity to allow bud formation prior to nuclear division. PMID: 23871241
    14. Cyclin-Cdk1-Cks1-dependent phosphorylation of multisite targets in Saccharomyces cerevisiae is controlled by key substrate parameters including distances between phosphorylation sites. PMID: 24186061
    15. The protein phosphatase PP2A(Cdc55) dephosphorylates APC/C, thereby counteracting the activity of the mitotic kinase Cdc28. PMID: 23999167
    16. Cdk1 phosphorylation inhibits the interaction of Exo84 with other exocyst components. PMID: 23836930
    17. Cdk1 modulates membrane-trafficking dynamics, which is likely to play an important role in coordinating cell surface growth with cell cycle progression. PMID: 22767578
    18. Phosphorylation of purified Clb2 by Cdk1 is inhibited by catalytically active Mck1 but not catalytically inactive Mck1 in vitro. PMID: 22918234
    19. Protein kinase A-mediated phosphorylation of Pah1p phosphatidate phosphatase functions in conjunction with the Pho85p-Pho80p and Cdc28p-cyclin B kinases to regulate lipid synthesis in yeast PMID: 22865862
    20. recruitment of Cdc28 and Kin28 to PMA1 is mutually dependent and that the activity of both kinases is required for full phosphorylation of C-terminal domain-Ser5, for efficient transcription, and for mRNA capping PMID: 22689984
    21. cell cycle kinases Cdc28 as central mediators of the cellular response to inhibition of fatty acid synthesis. (Cdc28) PMID: 22218487
    22. Cdk1 promotes kinetochore bi-orientation during recovery from spindle checkpoint arrest by restraining premature spindle elongation & extinguishing SAC signalling. Cdk1 is essential for sustaining the expression of Cdc20. PMID: 22056777
    23. PP2A(Cdc55) sets a threshold that limits activation of Wee1, thereby allowing a low constant level of Cdk1 activity to escape Wee1 inhibition in early mitosis. PMID: 21849476
    24. CDC28 is the major genetic determinant of meiotic and budding cell shape behaviors in the Ivory Coast variant of CDC28. PMID: 21527779
    25. Cdk1 promotes DSB repair by single-strand annealing and noncrossover recombination by acting mostly at the resection level PMID: 21901114
    26. Cyclin-dependent kinase (Cdk1) promotes efficient 5' strand resection at DNA double-strand breaks. PMID: 21841787
    27. Cdk1 and SUMO regulate Swe1 stability PMID: 21151918
    28. dephosphorylation of the Dsn1 kinetochore protein in metaphase requires Cdc14. PMID: 20923974
    29. for PAH1, we identified Ser(602), Thr(723), and Ser(744), which belong to the seven phosphorylation sites, as the sites phosphorylated by the CDC28 PMID: 21081492
    30. Cdk1 kinase counteracts unscheduled sumoylation of Srs2 and targets Srs2 to dismantle specific DNA structures. PMID: 20195513
    31. Repression of NHEJ at G(2) is achieved by efficient end resection and by the reduced association of core NHEJ proteins with DNA breaks, both of which depend on the CDK1 activity. PMID: 19699692
    32. DNA damage checkpoint activation by a double-strand break requires the cyclin-dependent kinase CDK1 (Cdc28) in budding yeast PMID: 15496928
    33. deregulation of S-CDK function has the potential to exacerbate genomic instability by reducing replication origin usage and eliciting vital Rad53-dependent checkpoint responses PMID: 15542831
    34. Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets PMID: 15744308
    35. Cdc28 and Ama1 function in a pathway to trigger Smk1-dependent steps in spore morphogenesis. PMID: 16079231
    36. interaction with Cln3 mediated by HPD (hydrophobic patch domain) of Cln3 PMID: 16200502
    37. Cdc28 activates Cdc5 via phosphorylation of threonine 242. PMID: 16303563
    38. Cdc28 directly regulates the formation of the DNA double-strand breaks that initiate recombination by phosphorylating the Mer2/Rec107 protein PMID: 16814718
    39. Completion of S phase and the activity of the CDK1 are required for telomere degradation. PMID: 17018298
    40. Cdk1 activity controls the timing of telomere elongation by regulating the single-strand overhang at chromosome ends. PMID: 17070718
    41. Cdc6p delays a specific subset of mitotic events and that Cdc28 inhibition is not sufficient to account for it. The depletion of Cdc6p in G(2)/M cells reveals that Cdc6p is rate limiting for the degradation of the APC/Cdc20 substrates Pds1p and Clb2p. PMID: 17130241
    42. Ime2 does not simply replace G1 cyclin-cyclin-dependent kinase complexes in promoting Sic1 degradation during meiosis PMID: 17137646
    43. The asymmetric localization of Kar9p to one spindle pole body and attached cytoplasmic microtubules is dependent on its interactions with microtubule-associated proteins and is regulated by the yeast Cdc28p PMID: 17251549
    44. NUP53 is a target of CDK1. PMID: 17461799
    45. The capability of cyclin-dependent kinases CDKA and CDKB from the green alga Chlamydomonas reinhardtii to complement a Saccharomyces cerevisiae cdc28 temperature-sensitive mutant, was investigated. PMID: 18421551
    46. Cdh1 inactivation by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle PMID: 18500339
    47. Cdc28 serves dual roles in inhibiting Cdh1-dependent anaphase-promoting complex (APC) activity during the cell cycle: stabilization of the Cdh1 inhibitor Acm1 and direct phosphorylation of Cdh1 to prevent its association with the APC. PMID: 18519589
    48. Swe1 may competitively inhibit Clb2-Cdc28 interactions with substrates, in addition to its well-known function as a regulator of CDK activity through tyrosine phosphorylation. PMID: 18558651
    49. cell-cycle control of DSB resection in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein PMID: 18716619
    50. The major yeast TG lipase Tgl4, the functional ortholog of murine adipose TG lipase ATGL, is phosphorylated and activated by cyclin-dependent kinase 1 (Cdk1/Cdc28). PMID: 19150427

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  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 数据库链接：

    KEGG: sce:YBR160W

    STRING: 4932.YBR160W