Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Saccharomyces cerevisiae Chromatin structure-remodeling complex subunit SFH1 (SFH1)

Recombinant Saccharomyces cerevisiae Chromatin structure-remodeling complex subunit SFH1 (SFH1)

  • 货号：
    CSB-YP313270SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP313270SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP313270SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP313270SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP313270SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SFH1
  • Uniprot No.：
  • 别名：
    SFH1; YLR321C; L8543.4; Chromatin structure-remodeling complex subunit SFH1; RSC complex subunit SFH1; SNF5 homolog 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MSHQNQLIPQ AYISNFHNRL TNEDDGIPIF TMAQQTRQHK RAKVVNYAEY DNDLFDEFNM NGSNFNNADT HYKDNAVSHE NTPALTNGVT MDGSEYNVLE NMNGADSIIS NNKYDAGSNM VVESLSGLNS NNNASNGPSN KAQAQDIGNA VLPDLQDQHH NPFNILRYPK IRDTFINGKV VSPYRLNTDQ ETKANANSGE AIMIPITLDI EHMGHTIKDQ FLWNYNDDSI SPEEFASIYC KDLDMTSATL QTQIANIIKE QLKDLENIAA TEIMSDLHVI INLTCNLQDR FFEDNFQWNL NDKSLTPERF ATSIVQDLGL TREFIPLISQ SLHETILKIK KDWVDGHLIQ DHVPNDAAFG YLSGIRLDID ELGSNWCPRV EILTKEEIQK REIEKERNLR RLKRETDRLS RRGRRRLDDL ETTMRM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression.
  • 基因功能参考文献：
    1. Data suggest that SFH1 and SEC14L undergo conformational changes as phosphatidylinositol/phosphatidylcholine bind to hydrophobic pockets/ligand binding sites. [review-like article] PMID: 25233419
    2. Results identify mutations that confer Sec14-like activities to a functionally inert pseudo-Sec14 (Sfh1), which seemingly conserves all of the structural requirements for Sec14 function. PMID: 21248202
  • 亚细胞定位：
    Nucleus. Note=Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.
  • 蛋白家族：
    SNF5 family
  • 数据库链接：

    KEGG: sce:YLR321C

    STRING: 4932.YLR321C