Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Saccharomyces cerevisiae Centromere-binding protein 1 (CBF1)

Recombinant Saccharomyces cerevisiae Centromere-binding protein 1 (CBF1)

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Centromere-binding protein 1(CBF1),Yeast
  • 货号：
    CSB-YP324599SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Centromere-binding protein 1(CBF1),Yeast
  • 货号：
    CSB-EP324599SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Centromere-binding protein 1(CBF1),Yeast
  • 货号：
    CSB-EP324599SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Centromere-binding protein 1(CBF1),Yeast
  • 货号：
    CSB-BP324599SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Centromere-binding protein 1(CBF1),Yeast
  • 货号：
    CSB-MP324599SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CBF1
  • Uniprot No.：
  • 别名：
    CBF1; CEP1; CP1; CPF1; YJR060W; J1730; Centromere-binding protein 1; CBP-1; Centromere promoter factor 1; Centromere-binding factor 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-351
  • 氨基酸序列
    MNSLANNNKL STEDEEIHSA RKRGYNEEQN YSEARKKQRD QGLLSQESND GNIDSALLSE GATLKGTQSQ YESGLTSNKD EKGSDDEDAS VAEAAVAATV NYTDLIQGQE DSSDAHTSNQ TNANGEHKDS LNGERAITPS NEGVKPNTSL EGMTSSPMES TQQSKNDMLI PLAEHDRGPE HQQDDEDNDD ADIDLKKDIS MQPGRRGRKP TTLATTDEWK KQRKDSHKEV ERRRRENINT AINVLSDLLP VRESSKAAIL ACAAEYIQKL KETDEANIEK WTLQKLLSEQ NASQLASANE KLQEELGNAY KEIEYMKRVL RKEGIEYEDM HTHKKQENER KSTRSDNPHE A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for chromosome stability and methionine prototrophy. It is involved in chromosomal segregation. Binds to a highly conserved DNA sequence (5'-RTCACRTG-3'), called CDEI, found in centromeres and in several promoters. DNA-binding activity is enhanced by MET28. Required as an auxiliary factor for transcriptional activation of sulfur metabolism together with MET4 and MET28.
  • 基因功能参考文献：
    1. Cbf1 is required for transcription at centromere DNA. PMID: 22000103
    2. Data suggest that the binding sites for Cbf1 and CEP3 (CBF3 subunit) are included within the centromeric nucleosome has important implications for models of the centromeric nucleosome and for kinetochore function. PMID: 21768332
    3. Cbf1p binding to the upstream sites was required for the binding of the ISW2 chromatin-remodeling complex to the Ino2p-Ino4p-binding sites on the INO1 promoter PMID: 20935143
  • 亚细胞定位：
    Nucleus. Mitochondrion. Chromosome, centromere.
  • 数据库链接：

    KEGG: sce:YJR060W

    STRING: 4932.YJR060W