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  4. Recombinant Saccharomyces cerevisiae Actin cytoskeleton-regulatory complex protein END3 (END3)

Recombinant Saccharomyces cerevisiae Actin cytoskeleton-regulatory complex protein END3 (END3)

  • 中文名称：
    酿酒酵母END3重组蛋白
  • 货号：
    CSB-YP327923SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母END3重组蛋白
  • 货号：
    CSB-EP327923SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母END3重组蛋白
  • 货号：
    CSB-EP327923SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母END3重组蛋白
  • 货号：
    CSB-BP327923SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母END3重组蛋白
  • 货号：
    CSB-MP327923SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    END3
  • Uniprot No.：
  • 别名：
    END3; YNL084C; N2307Actin cytoskeleton-regulatory complex protein END3; Endocytosis protein 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-349
  • 氨基酸序列
    MPKLEQFEIK KYWQIFSGLK PIENKVNHDQ VLPILYNSKL DSSVLNKIWF LADIDDDDNL DFEEFVICMR LIFDMVNKNI SSVPDELPDW LIPGSKVNLI KERKKRKQIE NADLPPKKEI KVDWYMSPDD LNQYEKIYNS CAKLTDGTIT FNELSTKLST KFFNISKTDL NKVWSLINPQ NLPSIDRDPT FYFIHCLRQR NDLGAEIPAS LPNSLAEVCN KKQLSYDLRS SQPPTKRKEE ANEVDNLRDN GQNSSSDSSG SNVLSNEDSI KQKYASLTDD QVANMREQLE GLLNYKKSEK TQGGSKLSKR INIRSITDDL DNIEQQVEVL ENYLNNKRHE LQALQAEIN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization. END3 regulates PAN1 function by preventing phosphorylation of PAN1 by PKR1 and is also involved in the correct localization of SLA1 to the cell cortex, in the bipolar budding of diploid cells and the correct distribution of chitin at the cell surface.
  • 基因功能参考文献：
    1. The C-terminal End3 Repeats of End3 associate with the N-terminal part of Pan1's central coiled-coil region. PMID: 24118836
    2. END3-mediated endocytosis is required for spore wall formation. PMID: 15944351
  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, actin patch. Note=Cytoplasmic and cortical actin patches.
  • 蛋白家族：
    END3 family
  • 数据库链接：

    KEGG: sce:YNL084C

    STRING: 4932.YNL084C