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  4. Recombinant Saccharomyces cerevisiae ADP-ribosylation factor GTPase-activating protein GCS1 (GCS1)

Recombinant Saccharomyces cerevisiae ADP-ribosylation factor GTPase-activating protein GCS1 (GCS1)

  • 中文名称：
    酿酒酵母GCS1重组蛋白
  • 货号：
    CSB-YP327405SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GCS1重组蛋白
  • 货号：
    CSB-EP327405SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GCS1重组蛋白
  • 货号：
    CSB-EP327405SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GCS1重组蛋白
  • 货号：
    CSB-BP327405SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GCS1重组蛋白
  • 货号：
    CSB-MP327405SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GCS1
  • Uniprot No.：
  • 别名：
    GCS1; YDL226CADP-ribosylation factor GTPase-activating protein GCS1; ARF GAP GCS1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-352
  • 氨基酸序列
    MSDWKVDPDT RRRLLQLQKI GANKKCMDCG APNPQWATPK FGAFICLECA GIHRGLGVHI SFVRSITMDQ FKPEELLRME KGGNEPLTEW FKSHNIDLSL PQKVKYDNPV AEDYKEKLTC LCEDRVFEER EHLDFDASKL SATSQTAASA TPGVAQSREG TPLENRRSAT PANSSNGANF QKEKNEAYFA ELGKKNQSRP DHLPPSQGGK YQGFGSTPAK PPQERSAGSS NTLSLENFQA DPLGTLSRGW GLFSSAVTKS FEDVNETVIK PHVQQWQSGE LSEETKRAAA QFGQKFQETS SYGFQAFSNF TKNFNGNAED SSTAGNTTHT EYQKIDNNDK KNEQDEDKWD DF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    GTPase-activating protein (GAP) for ARF1 and ARF2. Involved in intracellular vesicular transport. Required for transport from the trans-Golgi network. Implicated in the regulation of retrograde transport from the Golgi to the ER and in actin cytoskeletal organization. May be involved in the maintenance of mitochondrial morphology, possibly through organizing the actin cytoskeleton in Saccharomyces.
  • 基因功能参考文献：
    1. ALPS and the predicted AH region are involved in the regulation and function of Gcs1p by interacting with membrane phospholipids and vesicle proteins. PMID: 23564908
    2. Release of Arl1p from membranes in cells requires the hydrolysis of bound GTP, which is accelerated by Gcs1p. PMID: 15975906
    3. Taken together, these results indicate that GCS1 is essential for sporulation and suggest that GCS1 positively regulates SPO14. PMID: 16400173
    4. The ArfGAP Gcs1p accelerates vesicle (v)-target membrane (t)-SNARE complex formation in vitro, indicating that ArfGAPs may act as folding chaperones. PMID: 17522384
  • 亚细胞定位：
    Cytoplasm. Mitochondrion. Cytoplasm, perinuclear region. Golgi apparatus. Note=Found also in the mitochondria and in the perinuclear region.
  • 数据库链接：

    KEGG: sce:YDL226C

    STRING: 4932.YDL226C