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  4. Recombinant Saccharomyces cerevisiae 40S ribosomal protein S5 (RPS5)

Recombinant Saccharomyces cerevisiae 40S ribosomal protein S5 (RPS5)

  • 中文名称：
    酿酒酵母RPS5重组蛋白
  • 货号：
    CSB-YP326661SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RPS5重组蛋白
  • 货号：
    CSB-EP326661SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RPS5重组蛋白
  • 货号：
    CSB-EP326661SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RPS5重组蛋白
  • 货号：
    CSB-BP326661SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RPS5重组蛋白
  • 货号：
    CSB-MP326661SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RPS5
  • Uniprot No.：
  • 别名：
    RPS5; RPS2; YJR123W; J2045; 40S ribosomal protein S5; RP14; S2; Small ribosomal subunit protein uS7; YS8
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-225
  • 氨基酸序列
    SDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPII ERLTNSLMMN GRNNGKKLKA VRIIKHTLDI INVLTDQNPI QVVVDAITNT GPREDTTRVG GGGAARRQAV DVSPLRRVNQ AIALLTIGAR EAAFRNIKTI AETLAEELIN AAKGSSTSYA IKKKDELERV AKSNR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
  • 基因功能参考文献：
    1. Rps5 serine-223 phosphorylation is required for ribosomal small subunit maturation. PMID: 29352143
    2. Thus, remodeling of the uS7/eIF2alpha interface appears to stabilize first the open, and then the closed state of the eukaryotic pre-initiation complex to promote accurate AUG selection in vivo. PMID: 28169832
    3. The s conclude that the Rps5 beta-hairpin is as crucial as soluble initiation factors for efficient and accurate start codon recognition. PMID: 26134896
    4. the small ribosomal subunit (SSU) head component rpS5 and platform components are crucial components of a highly defined spatial arrangement in the head-platform interface of nascent SSUs, required for efficient processing of the predicted SSU rRNA 3' end PMID: 20419091
    5. Identification of the cis-elements required to support normal levels of transcription and accurate initiation from sites within the TATA-less and TFIID-dependent RPS5 core promoter. PMID: 20805245
    6. Analysis of the mutant yeast strains suggests that the N-terminal part of the rpS5 may impact the ability of 40S subunits to function properly in translation and affect the efficiency of initiation. PMID: 19969550
    7. Gln3-mediated transcription activated by nitrogen signal transduction is independent of Npr1 kinase and Rsp5-Bul1/2 ubiquitin ligase in Saccharomyces cerevisiae PMID: 16864574
    8. Negatively charged N-terminal extension of yeast rpS5 might affect the ribosomal recruitment of specific mRNAs. PMID: 17901157

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Universal ribosomal protein uS7 family
  • 数据库链接：

    KEGG: sce:YJR123W

    STRING: 4932.YJR123W