Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rhodobacter sphaeroides Intrinsic membrane protein pufX (pufX)

Recombinant Rhodobacter sphaeroides Intrinsic membrane protein pufX (pufX)

  • 货号：
    CSB-YP320220RLF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320220RLF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP320220RLF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320220RLF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320220RLF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pufX
  • Uniprot No.：
  • 别名：
    pufX; RHOS4_18590; RSP_0255; Intrinsic membrane protein PufX
  • 种属：
    Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-82
  • 氨基酸序列
    MADKTIFNDH LNTNPKTNLR LWVAFQMMKG AGWAGGVFFG TLLLIGFFRV VGRMLPIQEN QAPAPNITGA LETGIELIKH LV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Associated with the reaction center - light-harvesting complex I. May play a critical role in facilitating the interaction between this complex and other components required for light-driven cyclic electron transfer.
  • 基因功能参考文献：
    1. PufX R49L, PufX R53L, PufX R49/53L and PufX G52L do not affect assembly of PufX within the core or photosynthetic growth but they do prevent dimerization, consistent with predictions from the RC-LH1-PufX structure. The monomeric complex with PufX corresponds to one half of the dimer structure whereas LH1 completely encloses the RC if the gene encoding PufX is deleted. PMID: 28587931
    2. Data show that an alphaTrp-24 -->Phe alteration of the LH1 antenna impairs carotenoid binding and allows photosynthetic growth in the absence of PufX. PMID: 28130884
    3. Data show that energy trapping is more efficient when photosynthetic membranes consist of reaction center {RC)-light harvesting complex 1 (LH1)-PufX dimers and light harvesting complex 2 (LH2) complexes. PMID: 27013332
    4. N-terminus of PufX is processed at an early stage in the development of the photosynthetic membrane in Rhodobacter sphaeroides. PMID: 20937243
    5. structure of PufX in organic solvent reveals two hydrophobic helices flanked by unstructured termini and connected by a helical bend PMID: 17161397
    6. conformational analysis of RC-LH1-PufX core dimer of Rhodobacter sphaeroides PMID: 18326046

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane.
  • 蛋白家族：
    PufX family
  • 数据库链接：

    KEGG: rsp:RSP_0255

    STRING: 272943.RSP_0255