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  4. Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH)

Recombinant Rhizobium leguminosarum Nitrogenase iron protein (nifH)

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  • 中文名称：
    Recombinant Rhizobium leguminosarum Nitrogenase iron protein(nifH)
  • 货号：
    CSB-EP321828RKN
  • 规格：
    ￥2328
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    nifH
  • Uniprot No.：
  • 别名：
    nifH; Nitrogenase iron protein; EC 1.18.6.1; Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase; Fragment
  • 种属：
    Rhizobium leguminosarum
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    35.0 kDa
  • 表达区域：
    1-47aa
  • 氨基酸序列
    MAALRQIAFYGKGGIGKSTTSQNTLAALVDHHVPRIPMIIRIGGYAQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant R. leguminosarum nifH protein is encoded by the gene of nifH (1-47aa). The gene of nifH was cloned in a system (E.coli) that supported the expression of nifH and translation of messenger RNA. Modification of nifH by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 10xHis-GST tag & C-terminal Myc tag in the production. The purity is 85% determined by SDS-PAGE.

    nifH is a protein coding gene that encodes Nitrogenase iron protein. According to some research, nifH may have the following features.
    Through molecular evolution analysis of nifH gene sequence, significant diversity of nitrogen-fixing bacteria was found in rice roots. Comprehensive evaluation of PCR primer amplification of nitrase nifH gene. Improve the RFLP program to study the diversity of nifH genes in the nitrogen-fixing agent communities in the soil. Analysis of the complexity of the nifH gene pool in soil and garbage in Douglas Fir Forest Farm in the Karst Mountains of Oregon. The comparison of nifH gene pools in soil and soil microenvironment is completely different in nature. Amplification of the nifH gene of the marine cyanobacterium Trichodesmium thiebautii using degenerate oligonucleotides.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.
  • 蛋白家族：
    NifH/BchL/ChlL family