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  4. Recombinant Rat Survival motor neuron protein (Smn1)

Recombinant Rat Survival motor neuron protein (Smn1)

  • 中文名称：
    大鼠Smn1重组蛋白
  • 货号：
    CSB-YP021838RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Smn1重组蛋白
  • 货号：
    CSB-EP021838RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Smn1重组蛋白
  • 货号：
    CSB-EP021838RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Smn1重组蛋白
  • 货号：
    CSB-BP021838RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Smn1重组蛋白
  • 货号：
    CSB-MP021838RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Smn1; SmnSurvival motor neuron protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-289
  • 氨基酸序列
    MAMGSGGGAG SEQEDTVLFR RGTGQSDDSD IWDDTALIKA YDKAVASFKH ALKNGDMCET SDKPKGTARR KPAKKNKNQK KNATAPLKQW KAGDKCSAVW SEDGCVYPAT ITSVDLKRET CVVVYTGYGN KEEQNLSDLL SPTCEVANNT EQNTQENESQ VSTDDSEHSS RSLRSKAHSK SKAAPWTSFL PPPPPVPGAG LGPGKPGLRF SGPPPPPPPP PPFLPCWMPP FPSGPPIIPP PPPISPDCLD DTDALGSMLI SWYMSGYHTG YYMGFRQNKK EGKKCSHTN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
  • 基因功能参考文献：
    1. Report decreased number of motor neurons and a decrease of SMN1 protein expression in spinal cords of rats exposed to uranyl nitrate. PMID: 27153795
    2. Reduced SMN protein level alters miRNA expression and distribution in neurons. PMID: 25055867
    3. Involvement of SMN in formation of stress granules may play an important role in cell survival. PMID: 21234798
    4. RNAi-mediated degradation of endogenous Elk-1 resulted in elimination of the mcl-1 message PMID: 21362474
    5. The highest expression is associated with the cell clustering phase and expression of NMDA receptors PMID: 12030329
    6. Data show differential localization of the 18- and 23-kDa fibroblast growth factor 2 isoforms in the nuclei of rat Schwann cells, and that the 23-kDa isoform co-immunoprecipitates specifically with the survival of motor neuron protein. PMID: 12397076
    7. SMN interacts with non-methylated EWS and an EWS-derived RG-containing peptide. Symmetrical dimethylation of the EWS-derived RG-peptide increases the dissociation rate between SMN and the symmetrical dimethylated EWS RG-peptide. PMID: 14597228
    8. Data show that the rat survival motor neuron cDNA produces two isoforms with molecular weight of 32 and 35kDa, both localized in nuclear coiled bodies, but the 32kDa form is also cytoplasmic, whereas the 35kDa form is also microsomal. PMID: 15465016
    9. Forced expression of a-SMN isoform stimulates motor neuron axonogenesis in a time-dependent fashion and induces axonal-like growth in non-neuronal cells. PMID: 17261814
    10. selective overexpression of the SMN C-terminal domain promoted neurite outgrowth similar to full-length protein and could rescue the SMN knock-down effects PMID: 17317728
    11. Smn knockdown in PC12 cells alters the expression pattern of profilin II, resulting in an increase in the neuronal-specific profilin IIa isoform. PMID: 17873296

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  • 亚细胞定位：
    Nucleus, gem. Nucleus, Cajal body. Cytoplasm. Cytoplasmic granule. Perikaryon. Cell projection, neuron projection. Cell projection, axon. Cytoplasm, myofibril, sarcomere, Z line.
  • 蛋白家族：
    SMN family
  • 数据库链接：

    KEGG: rno:64301

    STRING: 10116.ENSRNOP00000024456

    UniGene: Rn.1119