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  4. Recombinant Rat Rabphilin-3A (Rph3a)

Recombinant Rat Rabphilin-3A (Rph3a)

  • 中文名称：
    大鼠Rph3a重组蛋白
  • 货号：
    CSB-YP020102RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Rph3a重组蛋白
  • 货号：
    CSB-EP020102RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Rph3a重组蛋白
  • 货号：
    CSB-EP020102RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Rph3a重组蛋白
  • 货号：
    CSB-BP020102RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Rph3a重组蛋白
  • 货号：
    CSB-MP020102RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rph3a
  • Uniprot No.：
  • 别名：
    Rph3a; Rabphilin-3A; Exophilin-1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-684
  • 氨基酸序列
    MTDTVVNRWM YPGDGPLQSN DKEQLQAGWS VHPGAQTDRQ RKQEELTDEE KEIINRVIAR AEKMETMEQE RIGRLVDRLE TMRKNVAGDG VNRCILCGEQ LGMLGSACVV CEDCKKNVCT KCGVETSNNR PHPVWLCKIC LEQREVWKRS GAWFFKGFPK QVLPQPMPIK KTKPQQPAGE PATQEQPTPE SRHPARAPAR GDMEDRRAPG QKPGPDLTSA PGRGSHGPPT RRASEARMST TTRDSEGWDH GHGGGAGDTS RSPGGEQGLR RANSVQASRP APASMPSPAP PQPVQPGPPG GSRAAPGPGR FPEQSTEAPP SDPGYPGAVA PAREERTGPT GGFQAAPHTA GPYSQAAPAR QPPPAEEEEE EANSYDSDQA TTLGALEFSL LYDQDNSNLQ CTIIRAKGLK PMDSNGLADP YVKLHLLPGA SKSNKLRTKT LRNTRNPVWN ETLQYHGITE EDMQRKTLRI SVCDEDKFGH NEFIGETRFS LKKLKANQRK NFNICLERVI PMKRAGTTGS ARGMALYEEE QVERIGDIEE RGKILVSLMY STQQGGLIVG IIRCVHLAAM DANGYSDPFV KLWLKPDMGK KAKHKTQIKK KTLNPEFNEE FFYDIKHSDL AKKSLDISVW DYDIGKSNDY IGGCQLGISA KGERLKHWYE CLKNKDKKIE RWHQLQNENH VSSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an essential role in docking and fusion steps of regulated exocytosis. At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release. In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Plays also a role in the exocytosis of arginine vasopressin hormone.
  • 基因功能参考文献：
    1. Findings indicate that Rph3A activity is linked to the aberrant synaptic localization of GluN2A-expressing NMDARs characterizing levodopa-induced dyskinesias; suggest that Rph3A/GluN2A complex could represent an innovative therapeutic target for those pathological conditions where NMDAR composition is significantly altered. PMID: 28823933
    2. Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in rat cells PMID: 14722103
    3. rabphilin, by interacting with alpha-actinin, organizes the cell cytoskeleton to facilitate granule localization within F-actin-rich regions PMID: 16043482
    4. Binding of rabphilin to SNAP-25 regulates exocytosis of synaptic vesicles after the readily releasable pool has either been physiologically exhausted by use-dependent depression, or has been artificially depleted by deletion of synaptobrevin. PMID: 16763567
    5. The crystal structure of the Ca2+-free C2A domain adopts the classical C2-domain fold consisting of an eight-stranded antiparallel beta-sandwich with type I topology, and contains conserved acidic residues responsible for calcium binding. PMID: 16790935
    6. These results indicate that the polybasic sequence in the rabphilin C2B domain functions as an effector domain for SNAP-25 and controls the number of 'releasable' vesicles docked to the plasma membrane. PMID: 17156129
    7. different PIP2 headgroup recognition modes suggest that PIP2 is a target of the C2A domain of rabphilin-3A while this phospholipid is an effector of the C2B domain PMID: 18434502
    8. Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A. PMID: 18945677
    9. Interactions of SNAP25A and RPH3A take place at cell surface during stimulated exocytosis. PMID: 18986604

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic cell membrane. Membrane; Peripheral membrane protein.
  • 组织特异性：
    Specifically expressed in brain.
  • 数据库链接：

    KEGG: rno:171039

    STRING: 10116.ENSRNOP00000001844

    UniGene: Rn.10976