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  4. Recombinant Rat Proto-oncogene tyrosine-protein kinase Src (Src)

Recombinant Rat Proto-oncogene tyrosine-protein kinase Src (Src)

  • 中文名称：
    大鼠Src重组蛋白
  • 货号：
    CSB-YP894363RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Src重组蛋白
  • 货号：
    CSB-EP894363RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Src重组蛋白
  • 货号：
    CSB-EP894363RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Src重组蛋白
  • 货号：
    CSB-BP894363RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Src重组蛋白
  • 货号：
    CSB-MP894363RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Src; Proto-oncogene tyrosine-protein kinase Src; EC 2.7.10.2; Proto-oncogene c-Src; pp60c-src; p60-Src
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-536
  • 氨基酸序列
    GSNKSKPKD ASQRRRSLEP AENVHGAGGA FPASQTPSKP ASADGHRGPN AAFVPPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGVTTFV ALYDYESRTE TDLSFKKGER LQIVNNTEGD WWLAHSLSTG QTGYIPSNYV APSDSIQAEE WYFGKITRRE SERLLLNAEN PRGTFLVRES ETTKGAYCLS VSDFDNAKGL NVKHYKIRKL DSGGFYITSR TQFNSLQQLV AYYSKHADGL CHRLTTVCPT SKPQTQGLAK DAWEIPRESL RLEVKLGQGC FGEVWMGTWN GTTRVAIKTL KPGTMSPEAF LQEAQVMKKL RHEKLVQLYA VVSEEPIYIV TEYMNKGSLL DFLKGETGKY LRLPQLVDMS AQIASGMAYV ERMNYVHRDL RAANILVGEN LVCKVADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS FGILLTELTT KGRVPYPGMV NREVLDQVER GYRMPCPPEC PESLHDLMCQ CWRKEPEERP TFEYLQAFLE DYFTSTEPQY QPGENL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN). When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1 and paxillin (PXN). In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). Another type of cell-cell junction, the gap junction, is also a target for SRC, which phosphorylates connexin-43 (GJA1). SRC is implicated in regulation of pre-mRNA-processing and phosphorylates RNA-binding proteins such as KHDRBS1. Also plays a role in PDGF-mediated tyrosine phosphorylation of both STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors. Involved in the RAS pathway through phosphorylation of RASA1 and RASGRF1. Plays a role in EGF-mediated calcium-activated chloride channel activation. Required for epidermal growth factor receptor (EGFR) internalization through phosphorylation of clathrin heavy chain (CLTC and CLTCL1) at 'Tyr-1477'. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Has a critical role in the stimulation of the CDK20/MAPK3 mitogen-activated protein kinase cascade by epidermal growth factor. Might be involved not only in mediating the transduction of mitogenic signals at the level of the plasma membrane but also in controlling progression through the cell cycle via interaction with regulatory proteins in the nucleus. Plays an important role in osteoclastic bone resorption in conjunction with PTK2B/PYK2. Both the formation of a SRC-PTK2B/PYK2 complex and SRC kinase activity are necessary for this function. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates DDR2 on tyrosine residues, thereby promoting its subsequent autophosphorylation. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-738'. Enhances DDX58/RIG-I-elicited antiviral signaling. Phosphorylates PDPK1 at 'Tyr-9', 'Tyr-373' and 'Tyr-376'. Phosphorylates BCAR1 at 'Tyr-226'. Phosphorylates CBLC at multiple tyrosine residues, phosphorylation at 'Tyr-341' activates CBLC E3 activity. Involved in anchorage-independent cell growth. Required for podosome formation. Mediates IL6 signaling by activating YAP1-NOTCH pathway to induce inflammation-induced epithelial regeneration.
  • 基因功能参考文献：
    1. The Na,K-ATPase-dependent Src kinase signaling changes with mesenteric artery diameter. PMID: 30142894
    2. src-family kinases-mediated inflammatory response may be responsible for central sensitization and chronic pain after lumbar disc herniation. PMID: 28952414
    3. show that the striatal-enriched protein tyrosine phosphatase (STEP) is recruited by Galphaq-coupled receptors, including the M1 muscarinic acetylcholine receptor (M1R), and opposes the Src tyrosine kinase-mediated increase in the function of NMDARs composed of GluN2A PMID: 27857196
    4. Our findings demonstrate that Na-K-ATPase regulates intercellular communication in the vascular wall via cSrc-dependent Cx43 tyrosine phosphorylation. PMID: 28122732
    5. In contrast to Na/K-ATPase alpha1, alpha3 could not regulate Src. Upon exposure to ouabain, Src activation did not occur, yet ERK was activated through Src-independent pathways involving PI3K and PKC. Hence, alpha3 expression confers signaling and pumping properties that are clearly distinct from that of cells expressing Na/K-ATPase alpha1. PMID: 27903584
    6. These data suggest that RANKL could regulate the expression of NFATc1 and cSrc mRNA in the marrow culture system. PMID: 27122093
    7. The enhancement of fear extinction induced by novelty exposure is accompanied by Src kinase phosphorylation and activation in a restricted time window. PMID: 26969275
    8. regulation of c-Src trafficking requires both microtubules and actin polymerization, and GEF-H1 coordinates c-Src trafficking, acting as a molecular switch between these two mechanisms PMID: 26866809
    9. identify and characterize a metabotropic NMDAR signaling complex comprised of NMDARs, Panx1 channels and Src kinase. PMID: 26854804
    10. 4-Hydroxynonenal stimulates Src tyrosine kinase in activation of the inflammation process PMID: 26466383
    11. Data suggest that members of the Src protein tyrosine kinase (PTK) family may be critical targets for the protection of the blood-brain barrier (BBB) following cerebral ischemia. PMID: 25269821
    12. Src and RhoA coordinate a regulatory network that determines cytoskeletal tension through activation of actomyosin contractility PMID: 24984203
    13. We.demonstrated that tyrosine c-Src-kinase plays the most important role in signal transduction from 5HT-receptors because its effects are specific with relation to these receptors. PMID: 25720289
    14. gastric mucosal inflammatory responses to H. pylori LPS by ghrelin elicits protein kinase C delta activation and leads to a marked increase in PI3K and cSrc phosphorylation on Ser that exerts the detrimental influence on the kinase activity PMID: 24840386
    15. c-Src promotes endocytosed Sertoli cell blood-testis barrier (BTB) proteins to endosome-mediated protein degradation for the degeneration of the old BTB. Sertoli cells engulf beads mimicking apoptotic germ cells via c-Src-mediated phagocytosis. PMID: 25117412
    16. Active PKCalpha phosphorylates Src to induce PI-3K to phosphorylate AKT that subsequently activates the ERK1/2 cascade to stimulate goblet cell proliferation. PMID: 23882690
    17. aldosterone nongenomically elevates Hsp90( alpha and beta ) protein expressions in rat kidney. Aldosterone had no effect on pc-Src protein levels but modulated localization. PMID: 23484111
    18. C-src plays an important role in regulating primordial follicle activation and growth via phosphatidylinositol 3-kinase (PI3K)-protein kinase C (PKC)-ERK1/2 pathway. PMID: 22905678
    19. Expression of mutant alpha1 Na/K-ATPase defective in conformational transition attenuates Src-mediated signal transduction PMID: 23288841
    20. Calcium-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors trigger neuronal nitric-oxide synthase activation to promote nerve cell death in an Src kinase-dependent fashion PMID: 22992730
    21. Activation of NMDA receptors during anoxia/ischemia recruits Src family kinases to open Panx1, leading to sustained neuronal depolarizations. PMID: 22956847
    22. Angiotensin (Ang)II receptors recruit c-Src, which is responsible for SHP-1 tyrosine-phosphorylation and activation that leads to cerebellar development and neuronal differentiation in rat hindbrain. PMID: 22120166
    23. During pulmonary ischemia, Src PTK activation, rather than alteration in gene expression, may play a critical role in reperfusion-induced lung injury. PMID: 22349424
    24. The lipid raft might play a role in mediating Japanese encephalitis virus-initiated Src signaling and tumor necrosis factor (TNF)-alpha/interleukin (IL)-1beta expression in microglia. PMID: 21354239
    25. Src brain expression gradually increases starting as early as 2 h after cerebral ischemia and remains high for 1 day. PMID: 21334414
    26. The regulatory function of Src and its modulation by PTP1B on oxidative phosphorylation in mitochondria. PMID: 21063895
    27. c-Src in paraventricular nucleus modulates sympathetic activity and cardiac sympathetic afferent reflex in renovascular hypertensive rats. PMID: 21340460
    28. Src and MLCK form a swelling-induced protein complex that regulates volume recovery through membrane turnover and compensatory endocytosis under osmotic stress. PMID: 21209319
    29. Endothelin-1 induces NADPH oxidase-driven superoxide generation through increase of p47(phox) protein expression. The signalling pathway for this effect involves c-Src activation and ERK1/2 phosphorylation. PMID: 20553682
    30. Data show that Src-mediated tyrosine phosphorylation of synapsin I increases its binding to synaptic vesicles and actin, and increases the formation of synapsin dimers, which are both potentially involved in vesicle clustering. PMID: 20530578
    31. critical role in oxidative phosphorylation and cell damage in cardiomyocytes PMID: 20385227
    32. Regulation of an ERG K+ current by Src tyrosine kinase. PMID: 11834728
    33. proto-oncogene c-src from rat testis was cloned PMID: 11913791
    34. results suggest that activation of c-Src kinase likely represents a critical step in mediating proliferative effects of both the precursor and fully processed forms of gastrins on intestinal epithelial cells PMID: 12488346
    35. interaction between Cas and p85 PI3K appears to be driven primarily by Src-dependent tyrosine phosphorylation of Cas PMID: 12692262
    36. H-Ras negatively regulates Src phosphorylation of NR2A and retention of NR2A into the synaptic membrane leading to inhibition of NMDA receptor function PMID: 12695509
    37. These findings suggest that active Src participates in platelet-derived growth factor B-chain-dependent regeneration of tubular cells from acute ischemic injury. PMID: 12819032
    38. Src kinase activity is induced by protein kinase C via activation of the protein tyrosine phosphatase PTP alpha PMID: 12826681
    39. c-src activation of PLCgamma is mediated by GIT1 PMID: 14523024
    40. c-src mediates sensitization of epidermal growth factor-induced signaling by bradykinin PMID: 14630916
    41. The SH3 domain of c-Src binds to the carboxyl terminal domain of Cx43 in a pH-dependent fashion. Increased affinity of c-Src for Cx43 at low pHi aids in separation of Cx43 from ZO-1 and facilitates internalization of Cx43. PMID: 14699011
    42. direct PKCalpha-c-Src-ERalpha interactions, which may be crucial in the modulation of estrogen responsiveness and the differentiation process in osteoblasts PMID: 14751567
    43. Src inhibitor PP-2 prevented activation of Src and p38(MAPK) and, consequently, proteolysis inhibition by insulin. PMID: 14985360
    44. O(2)(-) and related products play a role in mediating the effect of low K intake on c-Src expression and in suppressing ROMK channel activity and renal K secretion PMID: 15644319
    45. Aldosterone increased phosphorylation of c-Src, p38MAP kinase, and cortactin, a Src-specific substrate, in c-Src+/+, but not in c-Src-deficient, vascular smooth muscle cells. Nongenomic signaling by aldosterone occurs through c-Src-dependent pathways. PMID: 15699470
    46. The activation of c-Src by TGF-beta1 is EGFR dependent and is required for full Akt phosphorylation and cell survival PMID: 15856020
    47. c-Src, with its lipopolysaccharide induction, has an unperceived role in transmitting LPS signaling in macrophages. PMID: 15869794
    48. Physiological pathway from the cell matrix to increased prolactin gene transcription mediated by phosphatidylinositol 3-kinase and cytoskeletal RPTPalpha/Src/Rho/phosphatidylinositol 3-kinase and cytoskeletal changes. PMID: 15878970
    49. The study indicates that hGH stimulates rise in Ca(2+) and insulin secretion mainly through activation of the prolactin receptor and JAK2 and Src kinases in rat insulin-secreting cells. PMID: 16597920
    50. the Rho-mDia1 pathway regulates polarization and adhesion turnover by aligning microtubules and actin filaments and delivering Apc/Cdc42 and c-Src to their respective sites of action PMID: 16943426

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell junction, focal adhesion.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Isoform 2 is expressed in the brain with highest expression in the pyramidal layers of the hippocampus and the granular layer of the dentate gyrus and moderate expression in cortical regions, with higher levels in the superficial layers than in the deep l
  • 数据库链接：

    KEGG: rno:83805

    STRING: 10116.ENSRNOP00000012739

    UniGene: Rn.112600