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  4. Recombinant Rat Prohibitin (Phb)

Recombinant Rat Prohibitin (Phb)

  • 中文名称：
    大鼠Phb重组蛋白
  • 货号：
    CSB-YP017885RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Phb重组蛋白
  • 货号：
    CSB-EP017885RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Phb重组蛋白
  • 货号：
    CSB-EP017885RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Phb重组蛋白
  • 货号：
    CSB-BP017885RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Phb重组蛋白
  • 货号：
    CSB-MP017885RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Phb
  • Uniprot No.：
  • 别名：
    PhbProhibitin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-272
  • 氨基酸序列
    AAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIYTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus. Plays a role in adipose tissue and glucose Homeostasis in a sex-specific manner. Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells.; In the mitochondria, together with PHB2, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan. The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Regulates mitochondrial respiration activity playing a role in cellular aging. The prohibitin complex plays a role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates DDX58/RIG-I-mediated signal transduction and production of IFNB1 and proinflammatory cytokine IL6.; In the nucleus, acts as a transcription coregulator, enhances promoter binding by TP53, a transcription factor it activates, but reduces the promoter binding by E2F1, a transcription factor it represses. Interacts with STAT3 to affect IL17 secretion in T-helper Th17 cells.; In the plasma membrane, cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.
  • 基因功能参考文献：
    1. gonadotroph-in-dependent steroidogenesis in undifferentiated GCs are influenced not only by the expression of the key steroidogenic enzymes, but also by the 30-kDa PHB/pPHB, which acts as an obligatory mitochondrial protein that regulates mitochondrial function and, thus plays a central role as a key modulator of GC differentiation. PMID: 27044659
    2. results demonstrate that PHB confers cytoprotection against oxidative stress in ISO-induced hypertrophy and this process is associated with modulation of Akt/Gsk-3beta signaling mechanisms as evident from our PHB overexpression and knockdown experiments. PMID: 27854077
    3. Data suggest that prohibitin (PHB) may be a new therapeutic target for diabetic cardiomyopathy (DCM). PMID: 26623724
    4. Study shows that PHB is highly expressed in white adipose tissue and liver which expression is regulated by estrogen. PMID: 26048717
    5. prohibitin acts as a positive regulator of mechanisms that counteract oxidative stress and extracellular matrix accumulation and therefore has an antioxidative effect. PMID: 24185039
    6. PHB1 plays an important role in the development of SCI and might provide a therapeutic target to promote recovery from SCI. PMID: 25463519
    7. All-trans retinoic acid acts as a positive regulator of PHB1, PHB2 and ACE2, and as a negative regulator of ACE1, angiotensin I, and angiotensin II in a renal tubular epithelial cells model system under hypoxia/reoxygenation conditions. PMID: 25031298
    8. Prohibitin attenuates oxidative stress and extracellular matrix accumulation in renal interstitial fibrosis disease. PMID: 24204768
    9. Prohibitin inhibits apoptosis in granulosa cells through the ERK1/2 pathway and the Bcl2 and Bcl-xL. PMID: 24096434
    10. PHB1 and PHB2 expression are reduced in renal interstitial fibrosis; PHB1 positively correlates with PHB2 in renal interstitium. PMID: 23992168
    11. Prohibitin expression in different neuronal & non-neuronal cell populations in rat cell cultures showed strong expression in oligodendrocytes. It was localized to mitochondria & some cell nuclei. PMID: 22711522
    12. findings suggest that PHB is a novel negative regulator of FSH-induced steroidogenesis, and its action with chemerin may contribute to the dysregulation of steroidogenesis in the pathogenesis of polycystic ovarian syndrome PMID: 23254195
    13. Prohibitin down-regulation is associated with increased caspase-3 expression and cell apoptosis in renal interstitial fibrosis rats. PMID: 21914039
    14. Expression of prohibitin in cardiomyocytes under oxidative stress increased. Prohibitin translocated to mitochondria after oxidative stress. PMID: 21179762
    15. Prohibitin is an endogenous neuroprotective protein involved in ischemic tolerance. PMID: 22238093
    16. The increased prohibitin level in response to subtle caloric restriction (CR) suggests that this increase may be one of the early events leading to the expansion of lifespan in response to CR. PMID: 21256116
    17. Prohibitin is involved in oxidative stress signaling in the retina and retinal pigment epithelium. PMID: 20832420
    18. PHB is expressed in beta-cells, increases with oxidative stress and protects the cells against deleterious effects of ethanol. PMID: 20030709
    19. prohibitin is involved in thymic growth and may be important for maturation of T lymphocytes. PMID: 12639934
    20. Prohibitin has functional regulatory roles in granulosa and theca-interstitial cells and in the ovum during follicular maturation and atresia. PMID: 15028627
    21. prohibitin could serve an antiapoptotic role in undifferentiated granulosa cells PMID: 17038561
    22. PHB protein was positively expressed at normal renal tissues, strongly down-regulated in renal biopsy specimens, and negatively correlated with the expression of alpha-smooth-muscle actin (alpha-SMA) and with the degrees of tubulointerstitial lesions. PMID: 17043753
    23. Data indicate that prohibitin protects cardiomyocytes from oxidative stress-induced damage. PMID: 18958584

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  • 亚细胞定位：
    Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane.
  • 蛋白家族：
    Prohibitin family
  • 组织特异性：
    Expressed in brain, heart, intestine, kidney, liver, skeletal muscle. Highest levels in heart, liver, kidney and intestine. Also expressed in flagella of epididymal sperm. Expressed in lung tissue and pulmonary artery smooth muscle (at protein level).
  • 数据库链接：

    KEGG: rno:25344

    STRING: 10116.ENSRNOP00000066502

    UniGene: Rn.29754