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  4. Recombinant Rat Phosphatidylinositol 4-kinase type 2-alpha (Pi4k2a)

Recombinant Rat Phosphatidylinositol 4-kinase type 2-alpha (Pi4k2a)

  • 中文名称：
    大鼠Pi4k2a重组蛋白
  • 货号：
    CSB-YP860389RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pi4k2a重组蛋白
  • 货号：
    CSB-EP860389RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pi4k2a重组蛋白
  • 货号：
    CSB-EP860389RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pi4k2a重组蛋白
  • 货号：
    CSB-BP860389RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pi4k2a重组蛋白
  • 货号：
    CSB-MP860389RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pi4k2a; Pi4kiiPhosphatidylinositol 4-kinase type 2-alpha; EC 2.7.1.67; 55 kDa type II phosphatidylinositol 4-kinase; Phosphatidylinositol 4-kinase type II-alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-478
  • 氨基酸序列
    MDETSPLVSP ERAQPPEYTF PSVSGAHFPQ VPGGAVRVAA AGSGPSPPCS PGHDRERQPL LDRARGAAAQ GQTHTVAAQA QALAAQAAVA VHAVQTHRER NDFPEDPEFE VVVRQAEIAI ECSIYPERIY QGSSGSYFVK DSQGRIIAVF KPKNEEPYGN LNPKWTKWLQ KLCCPCCFGR DCLVLNQGYL SEAGASLVDQ KLELNIVPRT KVVYLASETF NYSAIDRVKS RGKRLALEKV PKVGQRFNRI GLPPKVGSFQ LFVEGYKDAD YWLRRFEAEP LPENTNRQLL LQFERLVVLD YIIRNTDRGN DNWLIKYDYP MDNPNCRDTD WVMVREPVIK VAAIDNGLAF PLKHPDSWRA YPFYWAWLPQ AKVPFSQEIK DLILPKISDP NFVKDLEEDL YELFKKDPGF DRGQFHKQIA VMRGQILNLT QALKDNKSPL HLVQMPPVIV ETARSHQRSS SESYTQSFQS RKPFFSWW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
  • 基因功能参考文献：
    1. defined the minimal catalytic domain and identified conserved amino acid residues required for catalysis PMID: 12215430
    2. This enzyme is a downstream target of neuronal calcium sensor-1 in enhancing exocytosis in neuroendocrine cells PMID: 12471042
    3. PI4KIIalpha is present on adaptor protein (AP) -3 organelles where it regulates AP-3 function PMID: 15944223
    4. PI4KIIalpha plays a role in the exocytotic process downstream of Ca(2+) signaling in antigen-mediated mast cell activation. PMID: 17122963
  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Membrane raft. Endosome. Cytoplasmic vesicle. Cell projection, dendrite. Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse, synaptosome. Mitochondrion. Membrane; Lipid-anchor. Cell membrane. Perikaryon. Cell projection, neuron projection.
  • 蛋白家族：
    PI3/PI4-kinase family, Type II PI4K subfamily
  • 组织特异性：
    Detected in adult brain, especially in neurons in the cerebellum, brain cortex, dorsal root ganglion and spinal cord (at protein level).
  • 数据库链接：

    KEGG: rno:114554

    STRING: 10116.ENSRNOP00000019874

    UniGene: Rn.162533