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  4. Recombinant Rat Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (Ngly1)

Recombinant Rat Peptide-N (4)- (N-acetyl-beta-glucosaminyl)asparagine amidase (Ngly1)

  • 中文名称：
    大鼠Ngly1重组蛋白
  • 货号：
    CSB-YP716830RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Ngly1重组蛋白
  • 货号：
    CSB-EP716830RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Ngly1重组蛋白
  • 货号：
    CSB-EP716830RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Ngly1重组蛋白
  • 货号：
    CSB-BP716830RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Ngly1重组蛋白
  • 货号：
    CSB-MP716830RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ngly1; Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; PNGase; EC 3.5.1.52; N-glycanase 1; Peptide:N-glycanase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-651
  • 氨基酸序列
    ASATLGSSS SSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPSDEK YRSIRIGNTA FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAVERR SRLDGSSQKV EFSQHPAAVR LPAEQPEDPT GLMQHSGNQP GQPLSLPSAP LVVGDSTIFK VLQSNIQHVQ LYENPVLQEK ALACIPVNEL KRKSQEKLFR ARKLDKGTKV SDEDFLLLEL LHWFKEEFFH WVNNVVCSRC GRETRSRDEA LPPNDDELKW GAKNVEDHYC DACQLSNRFP RYNNPEKLLE TRCGRCGEWA NCFTLCCRAL GFEARYVWDY TDHVWTEVYS PSQQRWLHCD ACEDVCDKPL LYEIGWGKKL SYIIAFSKDE VVDVTWRYSC KHEEVMSRRT KVKEELLRET INGLNKQRQL LLSESRRKEL LQRIIVELVE FISPKTPRPG ELGGRVSGSL AWRVARGETC LERKEILFIP SENEKISKQF HLRYDIVRDR YIRVSDNNAN ISGWENGVWK MESIFRKVEK DWNMVYLARK EGSSFAYISW KFECGSAGLK VDNVSIRTSS QSFETGSVRW KLRSEMAQVN LLGDRNLRSY DDFCGATEVT LEAELSRGDG DVAWQHTQLF RQSLNDHAEN GLEIIITFSD L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Transglutaminase-like superfamily, PNGase family
  • 数据库链接：

    KEGG: rno:361014

    STRING: 10116.ENSRNOP00000008289

    UniGene: Rn.108552