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  4. Recombinant Rat Pancreas/duodenum homeobox protein 1 (Pdx1)

Recombinant Rat Pancreas/duodenum homeobox protein 1 (Pdx1)

  • 中文名称：
    大鼠Pdx1重组蛋白
  • 货号：
    CSB-YP017746RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pdx1重组蛋白
  • 货号：
    CSB-EP017746RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pdx1重组蛋白
  • 货号：
    CSB-EP017746RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pdx1重组蛋白
  • 货号：
    CSB-BP017746RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pdx1重组蛋白
  • 货号：
    CSB-MP017746RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdx1
  • Uniprot No.：
  • 别名：
    Pdx1; Ipf1; Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; IPF-1; Islet/duodenum homeobox 1; IDX-1; Somatostatin-transactivating factor 1; STF-1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-283
  • 氨基酸序列
    MNSEEQYYAA TQLYKDPCAF QRGPVPEFSA NPPACLYMGR QPPPPPPPQF AGSLGTLEQG SPPDISPYEV PPLADDPAGA HLHHHLPAQL GLAHPPPGPF PNGTETGGLE EPSRVHLPFP WMKSTKAHAW KSQWAGGAYA AEPEENKRTR TAYTRAQLLE LEKEFLFNKY ISRPRRVELA VMLNLTERHI KIWFQNRRMK WKKEEDKKRS SGTTSGGGGG EEPEQDCAVT SGEELLALPP PPPPGGAVPS GVPAAAREGR LPSGLSASPQ PSSIAPLRPQ EPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
  • 基因功能参考文献：
    1. Results found that Pdx-1 overexpression exerts its effects on islet cell replication via a noncell-autonomous mechanism involving induction of a secreted soluble factor or factors. Also, Inhbb is identified as one factor mediating the proliferative effect of Pdx-1. PMID: 27620967
    2. restoring proteins in the diet after birth in low protein offspring dramatically impairs glucose homeostasis in early adulthood, by altering Pdx1 expression and downstream-target genes increasing the risk to develop type 2 diabetes. PMID: 25667428
    3. These results suggest that a secreted luciferase reporter system in vivo enables not only the monitoring of promoter activity but also a quantitative and minimally invasive assessment of physiological and pathological changes in small cell masses. PMID: 24029239
    4. It might promote both the proliferation and cytodifferentiation of small acinar cells and, thus, be closely related to acinar cell regeneration. PMID: 23123389
    5. PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells. PMID: 23853095
    6. B-cell translocation gene 2 (BTG2) is a crucial regulator in GLP-1-induced insulin gene expression and insulin secretion via upregulation of pancreatic duodenal homeobox-1 (PDX-1) in pancreatic beta-cells. PMID: 23703573
    7. a key role of PPARgamma in regulating pancreatic beta-cell function by improving the stability of Pdx1 and Mafa proteins PMID: 23424659
    8. Triiodothyronine (T3) induces proinsulin gene expression by activating PI3K: possible roles for GSK-3beta and the transcriptional factor PDX-1. PMID: 23147208
    9. Pdx1 expressing pancreatic progenitor cells preserve the viability and function of pancreatic islets. PMID: 22644623
    10. The inhibitory effect of Urotensin IIon insulin secretion is associated with a suppression of GCK expression, not regulation of PDX-1 expression. PMID: 20401447
    11. Nicotinamide mononucleotide can stimulate insulin secretion and upregulate the mRNA expression of PDX-1 in RIN-m5f cells. PMID: 22086006
    12. Through multiple mechanisms, SREBP-1c, when induced in a lipotoxic state, repressed PDX-1 expression contributing to the inhibition of insulin expression and beta cell dysfunction. PMID: 21652712
    13. PDX-1 and to a lesser extent other pancreatic transcription factors, could rapidly and specifically activate pancreatic lineage and beta-cell-like functional characteristics in ectoderm-derived human keratinocytes. PMID: 22028850
    14. AGEs induced nuclear accumulation of Foxo1; this in turn reduced Pdx-1 expression by decreasing its protein stability, ultimately affecting insulin synthesis PMID: 21533167
    15. IUGR rats that failed to undergo catch-up growth might be prone to abnormal glucose tolerance, decreases islet size, and increased PDX-1 mRNA in early adult life. PMID: 20626638
    16. Pax6 is localized in the nucleus and prevents the activation of the insulin gene by occupying the PDX-1 binding site and by interacting with BETA2 PMID: 20943817
    17. The purpose of this study was to confirm that the expression of Pdx1 could mediate the differentiation of rat mesenchymal stem cells (MSCs) into insulin-producing cells. PMID: 20306305
    18. AMPK regulates the expression of PDX-1 at both the transcriptional and protein levels, and PPARalpha may be acutely involved in the regulation of INS-1 cells. PMID: 20644547
    19. Roux-en-Y gastric bypass (RYGB) increases the expression of pancreatic PDX-1 and induces the regeneration of beta-cells. The associated regeneration of islet cells may be a possible mechanism that how RYGB could improve type 2 diabetes mellitus. PMID: 20458761
    20. the PDX1 C terminus has a role in mediating the effects of glucose, which modulates PDX1 stability via the AKT-GSK3 axis PMID: 19833727
    21. Data support the conclusion that the activation of Akt by ANP or 8-Br-cGMP promotes cyclin D2, PDX-1, and glucokinase transcription by phosphorylating and restricting Foxo1a activity. PMID: 19837876
    22. Combined expression of pancreatic duodenal homeobox 1 and islet factor 1 induces immature enterocytes to produce insulin. PMID: 11978636
    23. role in mediating GAD67 transcription in islets of Langerhans PMID: 12150938
    24. loss of PDX1 function alters expression of mitochondrially encoded genes through regulation of TFAM leading to impaired insulin secretion PMID: 15151993
    25. Pdx1 induces the expression of multiple genes related to endocrine pancreas development and islet function in these liver cells. PMID: 15561947
    26. Beta cell maturation accompanied by increased IL-1beta sensitivity is, in part, dependent upon the expression of genes regulated by Pdx1. PMID: 15605246
    27. prolonged exposure of pancreatic islets to palmitate inhibits insulin gene transcription by impairing nuclear localization of PDX-1 and cellular expression of MafA PMID: 15944145
    28. Expression of PDX-1, a repressor of sucrase and lactase promoters, was 4.5-fold higher in iron deficiency in rats. PMID: 16081762
    29. the pancreatic duodenal homeobox-1 protein is regulated by DNA-dependent protein kinase PMID: 16166097
    30. Data show that FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site. PMID: 16847327
    31. pancreatic duodenal homeobox (PDX)-1 expression decreased with age in pancreatic tissue sections of rats. These data support the use of islets from older rats as a more appropriate model to study glucose-induced beta-cell apoptosis. PMID: 16936193
    32. exogenous expression of Pdx-1 should provide a promising approach for efficiently producing islet-like cells from bone marrow mesenchymal stem cells for the future therapeutic use in diabetic patients PMID: 17172056
    33. Expression in pancreatic beta cells is enhanced by peroxisome proliferator-activated receptor-gamma. PMID: 17192469
    34. possible relationship between PDX-1 and the state of islet proliferation, islet-to-ductal transdifferentiation, apoptosis, and the expression of SSTRs PMID: 17583748
    35. PDX-1 expression may have a role in proliferation of duct epithelial cells after partial pancreatectomy in rats PMID: 17690043
    36. PDX-1 can differentiate rat pancreatic ductal epithelial cells into insulin-producing cells in vitro. In vitro PDX-1 transfection is a valuable strategy for increasing the source of insulin-producing cells. PMID: 17876894
    37. The expression of PDX-1 mRNA in islet samples of different purity suggests the presence of stem cells in all islet samples. PMID: 17897921
    38. progressive epigenetic silencing of Pdx1 has a role in development of type 2 diabetes following intrauterine growth retardation in rats PMID: 18464933
    39. Small hepatocytes were transformed into both insulin- and glucagon-producing cells following ectopic Pdx-1 gene expression. Mature hepatocytes were transformed predominantly into glucagon-producing cells. PMID: 18670842
    40. Data show that liver cells over-expressing Pdx-1 present interesting alterations concerning glucose metabolism. PMID: 18971862
    41. KGF directly induces the expression of PDX1 in some ductal cells thus inducing beta-cell neogenesis PMID: 19266047
    42. Chronic leucine might result in an increase in AMPK and then a decrease in PDX-l, in turn to depress GCK and GLUT2 resulting in decreased glucose-stimulated insulin secretion at high glucose and insulin content. PMID: 19438972
    43. In high-fat diet throughout gestation neonates, immunoreactivity of Pdx-1 was significantly reduced PMID: 19604517
    44. Insulin promoter factor 1 regulates transcription of mitochondrial DNA-encoded genes via regulation of mitochondrial factor A. PMID: 19656489

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    Antp homeobox family, IPF1/XlHbox-8 subfamily
  • 组织特异性：
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • 数据库链接：

    KEGG: rno:29535

    STRING: 10116.ENSRNOP00000066935

    UniGene: Rn.54603