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  4. Recombinant Rat PRKCA-binding protein (Pick1)

Recombinant Rat PRKCA-binding protein (Pick1)

  • 中文名称：
    大鼠Pick1重组蛋白
  • 货号：
    CSB-YP866801RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Pick1重组蛋白
  • 货号：
    CSB-EP866801RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Pick1重组蛋白
  • 货号：
    CSB-EP866801RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Pick1重组蛋白
  • 货号：
    CSB-BP866801RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Pick1重组蛋白
  • 货号：
    CSB-MP866801RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pick1; Prkcabp; PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-416
  • 氨基酸序列
    MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV QVFDNTPAAL DGTVAAGDEI TGVNGRSIKG KTKVEVAKMI QEVKGEVTIH YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI KKYLDVKFEY LSYCLKVKEM DDEEYSCIAL GEPLYRVSTG NYEYRLILRC RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA VLRDADVFPI EVDLAHTTLA YGPNQGGFTD GEDEEEEEED GAAREVSKDA RGATGPTDKG GSWCDS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.
  • 基因功能参考文献：
    1. Results suggest that the interacting proteins PICK1 and N-ethylmaleimide sensitive fusion protein act as regulators of presynaptic GluA2-containing AMPAR trafficking between the active zone and a vesicle pool that may provide the basis of presynaptic components of synaptic plasticity. PMID: 28057533
    2. PICK1 is a cargo-specific endocytic accessory protein required for efficient, activity-dependent AMPAR endocytosis. PMID: 28855251
    3. This study defines a novel mechanism whereby elevated [Ca(2+)] induced by NMDA receptor activation modulates Ago2 and miRNA activity via PICK1. Our work suggests a Ca(2+)-dependent process to regulate miRNA activity in neurons in response to the induction of long-term depression. PMID: 28404816
    4. syntabulin forms a complex with PICK1 and ASICs, regulates ASIC protein expression in neurons, and participates in ASIC-induced acidotoxicity. PMID: 26868290
    5. ICA1L forms BAR-domain complexes with PICK1 and is crucial for acrosome formation in spermiogenesis. PMID: 26306493
    6. PICK1 binds Rac1 and Cdc42, via distinct but overlapping binding sites PMID: 25475687
    7. PICK1 interacts with parkin and our data showed that PICK1 reduces PAELR expression levels in transiently transfected heterologous cells PMID: 24749734
    8. The Arp2/3 regulators PICK1, and N-WASP and WAVE2 function antagonistically to control the complexity of astrocyte branched morphology, and this mechanism underlies the morphological changes seen in astrocytes during their response to pathological insult. PMID: 23843614
    9. It is a binding partner of GHRHR and controls cell surface expression and signal activity of the receptor. PMID: 23823934
    10. Modulation of PICK1 regulates actin dynamics and synaptic function. PMID: 23889934
    11. a shift of PICK1 expression from motor neurones in healthy rats to activated astrocytes in animals with end-stage amyotrophic lateral sclerosis point to a potential implication of PICK1 in the progression of this disease PMID: 22624977
    12. The data of this study demonstrated that a PICK1-GRIP1-GluA2 complex is involved in the regulation of GluA2 trafficking after glycine stimulation and therefore suggest that this complex is involved in these processes during LTP expression. PMID: 22915106
    13. PICK1 is phosphorylated at Ser77 by PKCalpha preferentially when bound to membrane vesicles PMID: 22129425
    14. Via Arp2/3 inhibition, PICK1 has complementary yet distinct roles during LTD to regulate AMPA receptor trafficking and spine size, and therefore functions as a crucial factor in both structural and functional plasticity. PMID: 21252856
    15. Results indicate that PICK1 regulates trafficking and function of ASIC1a in a lipid binding-dependent manner. PMID: 21176140
    16. PICK1 may function in the regulation of synaptic transmission and/or plasticity. PMID: 12209850
    17. phosphorylation of p46 (PICK-1) by GTP and Ca(2+)/CaM may be important for the regulation of transporters and neurosecretion PMID: 12526085
    18. Surface expression of the netrin receptor UNC5H1 is regulated by Protein interacting with C kinase-1 in neurons. PMID: 14672991
    19. PICK1 has a role in ASIC2b regulation of ASIC3 PMID: 14976185
    20. These findings suggest that PICK1 acts to downregulate the GluR2 content of AMPARs at hippocampal CA1 synapses, thereby increasing synaptic strength at resting membrane potentials PMID: 15190111
    21. results define the structural basis for the unusual binding pattern of the PICK1 (protein interacting with C kinase 1) PDZ domain by substantiating the critical role of the alphaB1 position (Lysine(83)) PMID: 15774468
    22. Repetitive synaptic activity triggers loss of synaptic GluR2-lacking AMPARs by selectively disrupting their interaction with GRIP and that PICK drives activity-dependent delivery of GluR2-containing receptors PMID: 15895086
    23. Our results show that PICK1 localization in dendrites is subject to multiple forms of regulation that contribute to surface AMPAR expression, likely by modulating the numbers of AMPARs maintained in intracellular compartments. PMID: 16406232
    24. PICK1 synaptic targeting requires direct interaction between the PDZ domain and lipid membranes PMID: 17914463
    25. The switch from islet cell autoantigen 1 (ICA69)-PICK1 heteromeric complexes to PICK1-PICK1 homomeric complexes could be an important mechanism regulating synaptic targeting and surface expression of AMPA receptors. PMID: 18032668
    26. The trafficking of PICK1 from the cytoplasm to cell membrane is regulated by a single zinc2+-binding site. PMID: 18429931
    27. in the absence of PDZ ligand, the PICK1 BAR domain is inhibited through a PDZ domain-dependent and linker-dependent mechanism PMID: 18466293
    28. can suppress both the basal activity of the Arp2/3 complex as well as its activation by nucleation-promoting factors PMID: 18492472
    29. PICK1 is involved in the regulation of presynaptic AMPA receptor trafficking and in determining the size of the AMPA receptor pool that modulates presynaptic glutamate release. PMID: 19071197

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  • 亚细胞定位：
    Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Membrane; Lipid-anchor. Cell junction, synapse, postsynaptic density. Cell junction, synapse, synaptosome. Cytoplasm, cytoskeleton. Note=Also membrane-associated, present at excitatory synapses.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: rno:84591

    STRING: 10116.ENSRNOP00000016077

    UniGene: Rn.24750