Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat P2X purinoceptor 4 (P2rx4), partial

Recombinant Rat P2X purinoceptor 4 (P2rx4), partial

  • 中文名称：
    大鼠P2rx4重组蛋白
  • 货号：
    CSB-YP017322RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠P2rx4重组蛋白
  • 货号：
    CSB-EP017322RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠P2rx4重组蛋白
  • 货号：
    CSB-EP017322RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠P2rx4重组蛋白
  • 货号：
    CSB-BP017322RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠P2rx4重组蛋白
  • 货号：
    CSB-MP017322RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    P2rx4; P2X purinoceptor 4; P2X4; ATP receptor; Purinergic receptor
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
  • 基因功能参考文献：
    1. Olfactory ensheathing cell transplantation inhibits P2X4 receptor overexpression in spinal cord injury rats with neuropathic pain. PMID: 28461136
    2. Study found that spinal microglia were activated and that P2X4R was primarily expressed in spinal activated microglia during nicotine withdrawal (NTW). In addition, the P2X4R and BDNF expression levels in the spinal cord were upregulated during NTW. Furthermore, inhibition of microglial activation prevented thermal hyperalgesia and increased P2X4R and BDNF expression induced by NTW. PMID: 28306606
    3. These results indicated that TLR4 and P2X4R pathways mediated IL-1beta synthesis and release in microglia followed chronic morphine. PMID: 27506813
    4. The results suggest a role of PrP(C) in proteostasis, dysfunctions of which may be involved in the pathogenesis of neurodegenerative diseases such as TSE and Alzheimer's Disease. PMID: 26946358
    5. Alanine scanning mutagenesis of the R203-L214 (dorsal fin) and the D280-N293 (left flipper) sequences of the rat P2X4 receptor showed that ATP potency/efficacy was reduced in 15 out of 26 alanine mutants. PMID: 25398027
    6. ATP-binding-induced altered interdomain hydrophobic interactions and the concomitant coordinated motions of LF and DF domains are essential for the channel gating of P2X4. PMID: 24943126
    7. Specific localization of the P2X4 receptor subunit was evaluated in mouse, rat and cat retinae using fluorescence immunohistochemistry and pre-embedding immuno-electron microscopy PMID: 24997270
    8. NMR solution structure of the rat P2rx4 head domain. PMID: 25662851
    9. Resent research showed that the P2X4 receptor (P2X4R), an ATP-gated purinergic receptor expressed on microglia, drove tactile allodynia after nerve injury. PMID: 25074385
    10. Mechanical hypersensitivity involves an increase in the neuronal sensitivity to ATP in the skin, due to sensitization of P2X4 receptors. PMID: 24569146
    11. the lysosome-localized P2X4 may play specific roles in membrane trafficking of acidic organelles in mammalian cells. PMID: 24817123
    12. P2X4 receptors contribute to controlling the fate of activated microglia and its survival PMID: 24254916
    13. The results also suggest that the P2X3, but not P2X1, is the main subunit that mediates the fast ATP-activated current in nociceptive neurons. PMID: 23771671
    14. these results suggest that cysteines and disulfide bonds between cysteines are differentially involved in the potentiation of the rat P2X4 receptor by zinc. PMID: 23524095
    15. multiple roles of the extracellular vestibule amino acid residues in the P2X4R function. PMID: 23555667
    16. Data suggest that the monoclonal antibody is applicable as a tool for detecting increasing expression levels of the P2X4 receptor molecule accompanied with increasing intensity of neuropathic pain. PMID: 23220420
    17. CCL2 changed the distribution of lysosomes expressing P2X4 receptors in microglial cells. PMID: 22222817
    18. P2X4 receptor mediates ATP-induced activation of ameboid microglia cells and their production of proinflammatory cytokines. PMID: 22053919
    19. lateral mobility is P2X subunit- and cell-specific, increased in an ATP activation and calcium-dependent manner, and enhanced in activated microglia by the p38 MAPK pathway that selectively regulates slowly mobile receptors PMID: 22393055
    20. The three Panx1 isoforms and Panx2 form homomeric and heteromeric complexes in any combination. These splice forms can also physically associate with ATP-gated P2X2, P2X3, P2X4, and P2X7 receptor channels. PMID: 21907716
    21. Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes. PMID: 21844344
    22. P2 receptors, including P2X4R and P2X1R, might play roles in modulating the RV hypertrophy that occurs due to pulmonary hypertension in hypobaric hypoxia. PMID: 21378451
    23. Cross-talk between P2X4 and gamma-aminobutyric acid, type A receptors determines synaptic efficacy at a central synapse. PMID: 21482824
    24. Roles of conserved ectodomain cysteines of the rat P2X4 purinoreceptor in agonist binding and channel gating PMID: 20406028
    25. evidence that 2 types of purinoreceptors are functional in renal vascular smooth muscle cells: heteromeric P2X1/P2X4 receptors and monomeric P2X1 receptors PMID: 20590593
    26. Our results suggest that the expression of P2X4R in our model is modulated not by neuropathic pain, but by slight nerve injury. PMID: 20407394
    27. Results indicate that, at high hypotonic imbalances, swelling-induced ATP release activates the purinergic receptor P2X4, which in turn modulates the time course of VSOR Cl(-) current onset in a extracellular Ca(2+)-dependent manner. PMID: 20056605
    28. These results indicate that thyrotropin-releasing hormone-responsive cells, including lactotrophs, express homomeric and/or heteromeric P2X(4)Rs, which facilitate Ca(2+) influx and hormone secretion. PMID: 20009029
    29. Suggest that increased P2X4 and P2X7 receptor expression in pregnant rats is related to uterine contraction leading to term and preterm delivery. PMID: 19767540
    30. Histidine 140 plays a key role in the inhibitory modulation of the P2X4 nucleotide receptor by copper but not zinc PMID: 12819199
    31. pharmacological blockade of spinal P2X4 receptors (P2X4Rs), a subtype of ionotropic ATP receptor, reversed tactile allodynia caused by peripheral nerve injury without affecting acute pain behaviours in naive animals PMID: 12917686
    32. The ultrastructural study revealed that P2X(4)R immunoreactivity was present in neurones at both pre- and post-synaptic sites as well as in glial cell processes and somata. PMID: 15476696
    33. Molecular and biochemical evidence for presence of P2X receptors in cholangiocytes. P2X4 is likely primary isoform involved, representing a novel and functionally important component of purinergic signaling complex modulating biliary secretion. PMID: 15528255
    34. Taken together, these results suggest that the outer parts of TM1 and TM2 in P2X4 are helical and that they move during activation. PMID: 15795310
    35. Residual, lesional P2X4R+ cells implicate a putative role for activated microglia/macrophages and surviving neurons/neurites in the delayed onset of central neuropathic pain following rat spinal cord injury. PMID: 15885321
    36. Moderate cooling does not potentiate P2X-mediated vasoconstriction, but elevated temperature attenuates the vasoconstrictor response to P2X purinergic receptors. PMID: 15890753
    37. P2X4 receptor-specific residues contribute to the ivermectin effects on channel deactivation PMID: 16949036
    38. These results indicate that P2X(4)R as well as P2Y(12)R is involved in ATP-induced microglial chemotaxis. PMID: 17299767
    39. Lyn may be a critical kinase mediating nerve injury-induced P2X(4)R upregulation and neuropathic pain PMID: 17918263
    40. Data suggest that the P2X(4) receptor retains its function within the degradative environment of the lysosome and can subsequently traffic out of lysosomes to upregulate its exposure at the cell surface and phagosome. PMID: 17940064
    41. abnormal trafficking and proteolytic processing of the P2X(4) receptor protein may underlie neuron degeneration in amyotrophic lateral sclerosis PMID: 17990272
    42. Results identify and map residues of potential importance for P2X4 channel gating and interaction with ivermectin. PMID: 18427835
    43. These functional data support negative cross-talk between P2X(4) and rho1 receptors. PMID: 18675255
    44. The results suggested that ATP may activate p38 MAPK by binding to intrinsic P2X(4) receptors in microglia, and subsequently enhance the expression of P2X(4) receptors, contributing to spinal long-term potentiation. PMID: 18837052
    45. These data suggest that either heteromerization between P2X4 and P2X7 subunits results not in stable heteromeric complexes or P2X4/7 heteromers do not represent a dominant subtype in the tissues investigated. PMID: 18938136
    46. Significant changes in purinergic P2X receptor subtype expression in the hippocampus of rats studied 12 h after the onset of status epilepticus and during the chronic phase were found, including a decrease in P2X4 receptor expression during chronic phase. PMID: 19084381
    47. This study presents a picture whereby P2X(4)R become functional in response to initial phagocytic stimuli but return to a non-functional state during sustained activation by classical macrophage activation. PMID: 19283779
    48. P2X4R-evoked increase in divalent calcium ions (Ca2+) and activation of p38-MAPK leads to the synthesis and exocytotic release of BDNF from microglia. PMID: 19295157
    49. Homotrimers of P2X7 were able to co-immunoprecipitate with P2X4, suggesting that an interaction occurs between rather than within receptor complexes. PMID: 19304656
    50. The results suggest that cysteines and disulfide bonds between cysteines are differentially involved in the inhibition of the rat P2X4 receptor by ethanol. PMID: 19351603

    显示更多

    收起更多

  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    P2X receptor family
  • 组织特异性：
    Widespread distribution in the brain. Also expressed in epithelial cells.
  • 数据库链接：

    KEGG: rno:29659

    STRING: 10116.ENSRNOP00000001752

    UniGene: Rn.7176