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  4. Recombinant Rat Nuclear receptor subfamily 4 group A member 3 (Nr4a3)

Recombinant Rat Nuclear receptor subfamily 4 group A member 3 (Nr4a3)

  • 中文名称：
    大鼠Nr4a3重组蛋白
  • 货号：
    CSB-YP016064RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Nr4a3重组蛋白
  • 货号：
    CSB-EP016064RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Nr4a3重组蛋白
  • 货号：
    CSB-EP016064RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Nr4a3重组蛋白
  • 货号：
    CSB-BP016064RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Nr4a3重组蛋白
  • 货号：
    CSB-MP016064RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nr4a3
  • Uniprot No.：
  • 别名：
    Nr4a3; Nor1; Nuclear receptor subfamily 4 group A member 3; Neuron-derived orphan receptor 1/2; Nuclear hormone receptor NOR-1/NOR-2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-628
  • 氨基酸序列
    MPCVQAQYSP SPPGSTYATQ TYGSEYTTEI MNPDYAKLTM DLGSTGIMAT ATTSLPSFST FMEGYPSSCE LKPSCLYQMP PSGPRPLIKM EEGREHGYHH HHHHHHHHHH HHQQQQPSIP PPSGPEDEVL PSTSMYFKQS PPSTPTTPGF PPQAGALWDD ELPSAPGCIA PGPLLDPQMK AVPPMAAAAR FPIFFKPSPP HPPAPSPAGG HHLGYDPTAA AALSLPLGAA AAAGSQAAAL EGHPYGLPLA KRTATLTFPP LGLTASPTAS SLLGESPSLP SPPNRSSSSG EGTCAVCGDN AACQHYGVRT CEGCKGFFKR TVQKNAKYVC LANKNCPVDK RRRNRCQYCR FQKCLSVGMV KEVVRTDSLK GRRGRLPSKP KSPLQQEPSQ PSPPSPPICM MNALVRALTD ATPRDLDYSR YCPTDQATAG TDAEHVQQFY NLLTASIDVS RSWAEKIPGF TDLPKEDQTL LIESAFLELF VLRLSIRSNT AEDKFVFCNG LVLHRLQCLR GFGEWLDSIK DFSLNLQSLN LDIQALACLS ALSMITERHG LKEPKRVEEL CNKITSSLKD HQRKGQALEP SEPKVLRALV ELRKICTQGL QRIFYLKLED LVSPPSVIDK LFLDTLPF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that binds to regulatory elements in promoter regions in a cell- and response element (target)-specific manner. Induces gene expression by binding as monomers to the NR4A1 response element (NBRE) 5'-AAAAGGTCA-3' site and as homodimers to the Nur response element (NurRE) site in the promoter of their regulated target genes. Plays a role in the regulation of proliferation, survival and differentiation of many different cell types and also in metabolism and inflammation. Mediates proliferation of vascular smooth muscle, myeloid progenitor cell and type B pancreatic cells; promotes mitogen-induced vascular smooth muscle cell proliferation through transactivation of SKP2 promoter by binding a NBRE site. Upon PDGF stimulation, stimulates vascular smooth muscle cell proliferation by regulating CCND1 and CCND2 expression. In islets, induces type B pancreatic cell proliferation through up-regulation of genes that activate cell cycle, as well as genes that cause degradation of the CDKN1A. Negatively regulates myeloid progenitor cell proliferation by repressing RUNX1 in a NBRE site-independent manner. During inner ear, plays a role as a key mediator of the proliferative growth phase of semicircular canal development. Mediates also survival of neuron and smooth muscle cells; mediates CREB-induced neuronal survival, and during hippocampus development, plays a critical role in pyramidal cell survival and axonal guidance. Is required for S phase entry of the cell cycle and survival of smooth muscle cells by inducing CCND1, resulting in RB1 phosphorylation. Binds to NBRE motif in CCND1 promoter, resulting in the activation of the promoter and CCND1 transcription. Plays also a role in inflammation; Upon TNF stimulation, mediates monocyte adhesion by inducing the expression of VCAM1 and ICAM1 by binding to the NBRE consensus site. In mast cells activated by Fc-epsilon receptor cross-linking, promotes the synthesis and release of cytokines but impairs events leading to degranulation. Plays also a role in metabolism; by modulating feeding behavior; and by playing a role in energy balance by inhibiting the glucocorticoid-induced orexigenic neuropeptides AGRP expression, at least in part by forming a complex with activated NR3C1 on the AGRP-glucocorticoid response element (GRE), and thus weakening the DNA binding activity of NR3C1. Upon catecholamines stimulation, regulates gene expression that controls oxidative metabolism in skeletal muscle. Plays a role in glucose transport by regulating translocation of the SLC2A4 glucose transporter to the cell surface. Finally, during gastrulation plays a crucial role in the formation of anterior mesoderm by controlling cell migration. Also participates in cardiac hypertrophy by activating PARP1.
  • 基因功能参考文献：
    1. Loss of Nr4a1 and Nr4a3 impedes production of ATP and ultimately inhibits glucose-stimulated insulin secretion. These data demonstrate for the first time that the orphan nuclear receptors Nr4a1 and Nr4a3 are critical for beta-cell mitochondrial function and insulin secretion. PMID: 27221116
    2. mechanisms of NOR1 regulation PMID: 25451221
    3. Nkx6.1 and Nr4a receptors induce components of the anaphase-promoting complex, including ubiquitin-conjugating enzyme E2C, resulting in degradation of the cell cycle inhibitor p21 PMID: 24706823
    4. Over-expression of neuron-derived orphan receptor-1 (NOR-1) exacerbates neointimal hyperplasia after vascular injury PMID: 23390133
    5. differential expression of Nor1 may contribute to the differences in metabolic regulation between high running capacity (HCR) and low running capacity (LCR) phenotypes PMID: 22936731
    6. Baseline level of Nor-1 is higher in the Wistar-Kyoto rat model compared to comparison control Sprague-Dawley rats. PMID: 20851110
    7. platelet-derived growth factor-induced NOR1 transcription in smooth muscle cell is mediated through cAMP-response element-binding protein-dependent transactivation of the NOR1 promoter [NOR1] PMID: 16945922
    8. IEG c-fos is unaffected by suppression of calcineurin activity, the plasticity-related genes Egr1/Zif268 and Egr2/Krox-20 are up-regulated, and genes encoding the orphan nuclear hormone receptors Nor1 and Nur77 are down-regulated. PMID: 19270309
    9. Local muscle contractile activity is required for increased expressions of NR4A1 and NR4A3 mRNA during exercise muscle contractile activity-induced increases in NR4A1 and NR4A3 mRNA may be mediated by AMPK activation. PMID: 19359610
    10. Nr4a3 expression in the rat pineal gland increases 8-fold at night. Expression is under control of an adrenergic/cyclic AMP mechanism. PMID: 19103603

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR4 subfamily
  • 组织特异性：
    Expressed at high levels in cultured apoptotic neuronal cells and fetal brain, and at low level in adult brain.
  • 数据库链接：

    KEGG: rno:58853

    STRING: 10116.ENSRNOP00000008302

    UniGene: Rn.62694