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  4. Recombinant Rat Myelin basic protein (Mbp)

Recombinant Rat Myelin basic protein (Mbp)

  • 中文名称：
    大鼠Mbp重组蛋白
  • 货号：
    CSB-YP365771RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Mbp重组蛋白
  • 货号：
    CSB-EP365771RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Mbp重组蛋白
  • 货号：
    CSB-EP365771RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Mbp重组蛋白
  • 货号：
    CSB-BP365771RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Mbp重组蛋白
  • 货号：
    CSB-MP365771RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    MbpMyelin basic protein; MBP
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-195
  • 氨基酸序列
    ASQKRPSQR HGSKYLATAS TMDHARHGFL PRHRDTGILD SIGRFFSGDR GAPKRGSGKV PWLKQSRSPL PSHARSRPGL CHMYKDSHTR TTHYGSLPQK SQRTQDENPV VHFFKNIVTP RTPPPSQGKG RGLSLSRFSW GAEGQKPGFG YGGRASDYKS AHKGFKGAYD AQGTLSKIFK LGGRDSRSGS PMARR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I wanted to know what cell line was used to produce the MPB (ref number: CSB-MP365771RA).

A:
Thanks for your inquiry,The cell line we usually use is HEK293T cell.Except that, we could express the protein in CHO cell. If you need specific cell line, please let me know, and mark it on the P.O when place the order.Thanks.

靶点详情

  • 功能：
    Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
  • 基因功能参考文献：
    1. Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID: 29742816
    2. A role for the retrograde dynein/dynactin motor complex in anterograde mbp mRNA transport. PMID: 29073112
    3. The aberrant phase transition of MBP molecules from their cohesive to soluble and non-adhesive state is a mechanism triggering myelin breakdown in neuromyelitis optica. PMID: 27346352
    4. hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression PMID: 27230884
    5. Bilateral common carotid artery occlusion led to loss of myelin basic protein expression. PMID: 26670084
    6. Study demonstrated that downregulation of tau severely impairs process outgrowth, oligodendrocyte differentiation, and sheet formation, and that MBP mRNA transport to the distal cellular extensions is disturbed PMID: 25847153
    7. Protein levels of MBP were significantly greater in orbital frontal cortex (OFC) but not dorsal striatum (dSTR) of females as compared to males, suggesting that myelination is increased in OFC but similar in dSTR of females as compared to males PMID: 26002313
    8. MBP cell membrane localization is altered in the presence of galactolipids. PMID: 25003183
    9. Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4. PMID: 25512606
    10. The expression of myelin basic protein was detected in accordance with the radially extending myelinated processes of the neurons in the cortex. PMID: 25282817
    11. Given the principle of the longest time window and the lowest therapeutic dose, the optimized therapeutic dose and time window should be injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia in cerebral ischemic injury. PMID: 24524292
    12. The e-MBP isoform is exclusively present in OLN-93 cells. PMID: 24321769
    13. MBP epitopes normally sheltered from immunosurveillance may induce MBP-specific T cell clones and a self-sustaining immune reaction, which may together contribute to transition of acute pain into chronic neuropathic pain state. PMID: 22676642
    14. Methylmercury-induced perturbation in Mbp gene expression occurred as a consequence of decreased oligodendrocyte cell population in absence of a significant impact on its post-transcriptional regulation. PMID: 22835759
    15. Morphological basis of spinal cord recovery from debilitating experimental neurodenerative disease is evidenced by robust myelin basic protein (MBP) expression and extensive remyelination. PMID: 21996274
    16. The impact of Hoxa2 over-expression on the proliferation and differentiation of CG4-SHoxa2 cells was investigated. PMID: 21479584
    17. MBP synthesis is regulated by hnRNP-K and integrin activation. PMID: 21357748
    18. Myelin basic protein expression decreases following demyelination of optic nerve and chiasm and then increases to control levels 14 days post-lesion. PMID: 20637745
    19. Sp1 phosphorylation can be regulated by PKC-ERK pathways. This phosphorylation is important for MBP transcription and oligodendrocyte differentiation PMID: 20882567
    20. Increasing the negative surface charge of the cell membrane reduces the amount of Fyn-SH3 domain binding to membrane-associated MBP. PMID: 19178193
    21. Phosphatidylinositol-3 kinase p85 enhances expression from the myelin basic protein promoter in oligodendrocytes independent of PI3K activity and dependent on the adapter functions of the p85 subunit's SH2 domains PMID: 12390518
    22. isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform. The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. PMID: 14580679
    23. Two regulatory elements in the promoter interact with Nkx2.2 in vitro PMID: 15695521
    24. Susceptibility or resistance to EAE is not regulated by the amount of the neuroantigens expressed in the thymus. Furthermore, the lesion distribution, but not the clinical course, of EAE is related to the neuroantigen expression in the CNS. PMID: 16773652
    25. Mbp is a binding partner and calmodulin adaptor for the BKCa channel. PMID: 17610306
    26. 21.5 kDa molecule expressed during [dental] hard tissue formation PMID: 18821983
    27. study demonstrates co-localisation of MBP & myristoylated alanine-rich C kinase substrate (MARKS) in oligodendrocytes, & co-distribution of MBP, MARCKS & GAP-43 in lipid raft fractions recovered from myelin & brain homogenates PMID: 19026719
    28. Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis. PMID: 19211156

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  • 亚细胞定位：
    Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
  • 蛋白家族：
    Myelin basic protein family
  • 组织特异性：
    Found in both the central and the peripheral nervous system.
  • 数据库链接：

    KEGG: rno:24547

    STRING: 10116.ENSRNOP00000022303

    UniGene: Rn.203146