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  4. Recombinant Rat Mitogen-activated protein kinase 8 (Mapk8)

Recombinant Rat Mitogen-activated protein kinase 8 (Mapk8)

  • 中文名称：
    大鼠Mapk8重组蛋白
  • 货号：
    CSB-YP013466RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Mapk8重组蛋白
  • 货号：
    CSB-EP013466RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Mapk8重组蛋白
  • 货号：
    CSB-EP013466RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Mapk8重组蛋白
  • 货号：
    CSB-BP013466RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Mapk8重组蛋白
  • 货号：
    CSB-MP013466RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mapk8; Jnk1; Prkm8; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; EC 2.7.11.24; SAPK gamma; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; p54 gamma
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-411
  • 氨基酸序列
    MSRSKRDNNF YSVEIADSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC LKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP VSSPSVNDMS SMSTDPTLAS D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly.
  • 基因功能参考文献：
    1. Autophagy protects bone marrow mesenchymal stem cells from palmitate-induced apoptosis through the reactive oxygen speciesJNK/p38 MAPK signaling pathways. PMID: 29901107
    2. The JNK signaling pathway is involved in regulating trypsinogen activation in rat pancreatic AR42J cells. SP600125 is an ATPcompetitive, efficient, selective and reversible inhibitor of JNK. the results were verified by four sets of experiments and demonstrated that trypsinogen activation is mediated by the JNK signaling pathway in the pathogenesis of acute pancreatitis PMID: 29207022
    3. Ischemia/reperfusion-induced JNK phosphorylation was reduced by SP600125, indicating that JNK mediates the apoptosis pathways in rat skin. PMID: 27884327
    4. JNK1 activation plays a role in the biological link between depression and NASH. Sitagliptin significantly decreased expression of hepatic JNK1 in rat model of NAFLD with mild chronic stress. PMID: 27916420
    5. JNK1 mediates pancreatic beta apoptotic cell death associated with increased Myc expression in IL1-beta treated cells. PMID: 26962537
    6. Inhibition of JNK by hydrogen-rich saline showed beneficial effects on LPS-challenged rats, at least in part, by restoring cardiac fatty acid oxidation PMID: 26565704
    7. Data show that in vivo inhibition of c-jun N-terminal kinase (JNK) activation resulted in a significant and sustained decrease in apoptosis in the ganglion cell layer, the inner nuclear layer and the photoreceptor layer. PMID: 26427475
    8. JNK-dependent LMO7b phosphorylation is required for hypercapnia-mediated Na,K-ATPase endocytosis. PMID: 26370512
    9. spinal JNK activity is dependent on TNF and that both TNF and the JNK signalling pathways modulate pain-like behaviour induced by peripheral inflammation PMID: 24942612
    10. These findings indicate that CORM-3 pretreatment interferes with JNK/AP-1 signaling and suppresses LPS-induced upregulation of the proadhesive phenotype in hCMEC/D3. PMID: 25098198
    11. Quantitative proteomics reveals dynamic interaction of JNK with RNA transport granule proteins Sfpq and Nono during neuronal differentiation PMID: 25326457
    12. Nymphaea rubra ameliorates TNF-alpha-induced insulin resistance via suppression of c-Jun NH2-terminal kinase and nuclear factor-kappaB in the rat skeletal muscle cells. PMID: 25234391
    13. These findings demonstrate that mitochondrial JNK activation, rather than JNK mitochondrial localization, induces autophagy and apoptosis and exacerbates myocardial ischemia/reperfusion injury. PMID: 24859228
    14. JNK1 protects against glucolipotoxicity-mediated beta-cell apoptosis. PMID: 24475223
    15. identified NMI induction as a novel negative feedback mechanism that decreases IRE1alpha-dependent activation of JNK and apoptosis in cytokine-exposed beta cells PMID: 24936061
    16. Data suggest that, in skeletal muscle, mTORC1 (mechanistic target of rapamycin, complex 1)/JNK signaling coordinates site-specific phosphorylation of p70S6K1 (ribosomal protein S6 kinase polypeptide 1); mTORC1/mTORC2 play roles in inhibition of JNK. PMID: 24801387
    17. Analgesic effect of acupuncture is mediated via inhibition of JNK activation in astrocytes after spinal cord injury. PMID: 24040124
    18. JNK1 and c-Jun were shown to be major targets of DCA. PMID: 24421392
    19. Elevated homocysteine induced the apoptosis of bone marrow mesenchymal stem cells via reactive oxygen species-induced the activation of JNK signal. PMID: 23667638
    20. Rac-1 superactivation restores the GLUT4 response in muscle cells rendered insulin-resistant via JNK activation or ceramide delivery. PMID: 23640896
    21. All-trans retinoic acid suppressed the mRNA expression of JNK and AP-1, which inhibited proliferation and collagen production of hepatic stellate cells. PMID: 21181362
    22. In serum starved RGC-5 cells, upregulation of JNK1 and the nuclear translocation and activation of phospho-JNK implies its importance in normal tension glaucoma. PMID: 22775755
    23. JNK activation in the spinal cord is required in the maintenance of cancer-induced bone pain. PMID: 22681856
    24. Exercise could increase muscle responsiveness to insulin, improving the total JNK and p-JNK and mRNA expression. PMID: 21186629
    25. data provide initial proof-of-concept that the neuronal death target JNK may also participate in endogenous processes of neurovascular remodeling and recovery after cerebral ischemia. PMID: 22699892
    26. JNK1/2 activation is associated with endogenous NO in response to ischemic insult. PMID: 22088537
    27. Data show that bulbospinal neuron populations react differently to injury and that the cell body response of spared neurons is distinct from that of axotomized neurons since they can up-regulate c-Jun but not HSP27. PMID: 21132399
    28. c-Jun induction is independent of Egr during apoptosis induced by activity deprivation in cerebellar granule neurons. PMID: 22143198
    29. A novel JNK1-Hes-1 signaling pathway is identified that regulates GluR1 expression involved in synaptic function in cortical neurons. PMID: 22302822
    30. IL-1 beta-induced collagen synthesis may be mediated by cytoplasmic JNK and p38/AP-1 pathways. PMID: 22229265
    31. The level of JNK and c-Jun phosphorylation increases obviously in lungs of rats with paraquat poisoning. PMID: 19080378
    32. Phosphorylation of JNK is closely related to asthma airway remodeling. Glucocorticoids can inhibit phosphorylation of JNK. PMID: 20450637
    33. Data suggest that K252a induced neuroprotection against ischemia/reperfusion in rat hippocampal CA1 subregion via inhibiting the mitochondrial Bax apoptosis pathway induced by JNK/MLK3 activation. PMID: 21726169
    34. Dexmedetomidine-induced contraction involves a JNK- and p38 MAPK-mediated pathway downstream of alpha-adrenoceptor stimulation in rat aortic smooth muscle. PMID: 21488184
    35. JNK MAPK is involved in the expression of endothelial cell adhesion molecules in thermal injury. PMID: 21263381
    36. phosphorylation of ATF-2 by JNK is associated with retinal ganglion cell death after axon damage. PMID: 21300140
    37. Data show that neonatal overweight increased hypoxia-ischemia (HI)-induced neuronal apoptosis, microglial activation and BBB damage, and aggravated HI brain damage in rat pups through JNK hyperactivation. PMID: 21518436
    38. Peptidoglycan-induced IL-6 expression is mediated by AP-1 activation through the TLR2 and JNK/c-Jun pathways in microglia. PMID: 20945380
    39. Data suggest that inhibition of spinal c-Jun N-terminal kinase activation may be involved in the analgesic effects of ketamine on spinal nerve ligation-induced neuropathic pain. PMID: 21255465
    40. Ischemic postconditioning can inhibit phosphorylation of JNK MAPK, which attenuates cardiocyte apoptosis. PMID: 20014487
    41. PI3K/Akt had intimate association with JNK and ERK activation in Tamoxifen-induced apoptosis. PMID: 21042752
    42. Expression of P-c-Jun in rats is increased during asthma airway remodeling. PMID: 19099816
    43. PI3-kinase, Rac1, and JNK were essential for bFGF-induced fibroblast migration PMID: 20808927
    44. HNE may not be just a passive biomarker of hepatic oxidant stress but rather an active mediator of hepatocellular injury through effects on JNK signaling PMID: 20501438
    45. Danshensu showed an obvious inhibitory effect on JNK phosphorylation in hepatic stellate cells induced by interleukin-1beta. PMID: 19460707
    46. Data show that Cdk5 as a major regulator of the JNK cascade, and suggest that Cdk5 is a preferable therapeutic target for Alzheimer disease relative to JNK and c-Jun. PMID: 19776350
    47. Total jun N-terminal kinase (JNK) and phosphorylated JNK are reduced following undernutrition in the cerebral cortex of newborn rats. PMID: 19840222
    48. There is cross-talk between the calcineurin and JNK pathway in controlling renovascular hypertension-induced cardiac hypertrophy. PMID: 18957384
    49. results demonstrate a significant role for JNK1 in A beta-mediated induction of the apoptotic cascade in cultured cortical neurons PMID: 12534344
    50. investigated the roles of G alphai, G alphaq, G alpha12/G alpha13, and G betagamma subunits on endothelin-1-induced JNK and ERK activation in cultured rat neonatal myocytes; results clearly demonstrate that differential pathways activate JNK and ERK PMID: 12606754

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell junction, synapse.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 数据库链接：

    KEGG: rno:116554

    UniGene: Rn.4090