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  4. Recombinant Rat Mitogen-activated protein kinase 10 (Mapk10)

Recombinant Rat Mitogen-activated protein kinase 10 (Mapk10)

  • 货号：
    CSB-YP013449RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013449RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013449RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013449RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013449RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mapk10; Jnk3; Prkm10Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; EC 2.7.11.24; SAPK-beta; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; p54-beta
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-464
  • 氨基酸序列
    MSLHFLYYCS EPTLDVKIAF CQGFDKHVDV SSVVKHYNMS KSKVDNQFYS VEVGDSTFTV LKRYQNLKPI GSGAQGIVCA AYDAVLDRNV AIKKLSRPFQ NQTHAKRAYR ELVLMKCVNH KNIISLLNVF TPQKTLEEFQ DVYLVMELMD ANLCQVIQME LDHERMSYLL YQMLSAIKHL HSAGIIHRDL KPSNIVVKSD CTLKILDFGL ARTAGTSFMM TPYVVTRYYR APEVILGMGY KENVDIWSVG CIMGEMVRHK ILFPGRDYID QWNKVIEQLG TPCPEFMKKL QPTVRNYVEN RPKYAGLTFP KLFPDSLFPA DSEHNKLKAS QARDLLSKML VIDPAKRISV DDALQHPYIN VWYDPAEVEA PPPQIYDKQL DEREHTIEEW KELIYKEVMN SEEKTKNGVV KGQPSPSGAA VNSSESLPPS SSVNDISSMS TDQTLASDTD SSLEASAGPL GCCR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1.
  • 基因功能参考文献：
    1. A novel interaction between KLF9 and JNK3, contributes to axon growth suppression and inhibition of axon regeneration. PMID: 28871032
    2. Results suggest that cilostazol could protect hippocampal neurons and ameliorate the impairment of learning/memory abilities and locomotor/exploratory activities in ischemic stroke via a PI3K-Akt1/JNK3/caspase-3 dependent mechanism PMID: 27769787
    3. In hippocampal neurons, BDNF up-regulates JIP3 expression via CREB activation. PMID: 26303065
    4. JNK3 phosphorylatesrRat brain mitochondrial VDAC channel, which leads to the channel closure. PMID: 25721670
    5. Rotenone induces dopamine neuron death through a series of sequential events including microtubule destabilization, JNK3 activation, VMAT2 inhibition, accumulation of cytosolic dopamine, and generation of ROS. PMID: 25496994
    6. JNK3 participates in an adaptive response to neuronal hyperexcitation by impeding secretory trafficking at the Golgi complex. PMID: 23838184
    7. Akt1 activation is involved in the neuroprotection of HSP72 against ischemic brain injury via suppressing JNK3 signaling pathway, providing a new experimental foundation for stroke therapy. PMID: 22386689
    8. Data show that JNK3 silencing strongly decreases Insulin Receptor Substrate 2 (IRS2) protein expression. PMID: 22563476
    9. Data suggest that JNK isoforms are involved in differential regulation of neuronal death and regeneration, and that JNK3 appears to be required for regeneration and survival in the case of an environment permissive for regeneration. PMID: 21468718
    10. K252a plays a neuroprotective role in ischemic injury via inhibition of the JNK pathway (MKK7 AND JNK3) PMID: 15680699
    11. interaction of free arrestins with JNK3 and Mdm2 and their ability to regulate subcellular localization of these proteins may play an important role in the survival of photoreceptors and other neurons PMID: 16737965
    12. These findings strongly suggest that Cdc42 serves as an upstream activator and modulates JNK-mediated apoptosis machinery in vivo, which ultimately results in neuronal apoptosis via nuclear and non-nuclear pathways. PMID: 17161586
    13. JNK3 apoptotic signaling pathway is attentuated by stimulating GABA receptors via inhibiting the increased assembly of the GluR6-PSD-95-MLK3 signaling module in cerebral ischemia PMID: 18307989
    14. A prominent role for JNK3 is the maintenance of cellular differentiation in glia cells; expression of JNK3 protein and mRNA are increased in growth arrested C6 glioma cells. PMID: 18513991
    15. In a neonatal rat model of pneumococcal meningitis, JNK3 (but not JNK1 or JNK2) is activated in the hippocampus during the acute phase of infection and may be involved in processes unrelated to apoptosis in this disease. PMID: 18703144

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  • 亚细胞定位：
    Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 数据库链接：

    KEGG: rno:25272

    STRING: 10116.ENSRNOP00000060904

    UniGene: Rn.9911