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  4. Recombinant Rat Guanine nucleotide-binding protein subunit beta-2-like 1 (Gnb2l1)

Recombinant Rat Guanine nucleotide-binding protein subunit beta-2-like 1 (Gnb2l1)

  • 中文名称：
    大鼠Rack1重组蛋白
  • 货号：
    CSB-YP009605RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Rack1重组蛋白
  • 货号：
    CSB-EP009605RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Rack1重组蛋白
  • 货号：
    CSB-EP009605RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Rack1重组蛋白
  • 货号：
    CSB-BP009605RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Rack1重组蛋白
  • 货号：
    CSB-MP009605RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rack1
  • Uniprot No.：
  • 别名：
    Rack1; Gnb2l1; Receptor of activated protein C kinase 1; Guanine nucleotide-binding protein subunit beta-2-like 1; Receptor for activated C kinase; Receptor of activated protein kinase C 1) [Cleaved into: Receptor of activated protein C kinase 1; N-terminally processed]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-317
  • 氨基酸序列
    MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QVTIGTR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane.
  • 基因功能参考文献：
    1. Endoplasmic reticulum stress preconditioning protects against hepatic ischemia/reperfusion injury, which is orchestrated by IRE1-RACK1 axis through the activation of BCL2. PMID: 26711306
    2. RACK1 regulates angiotensin II induced contractions of preglomerular vascular smooth muscle in spontaneously hypertensive rats. PMID: 28100502
    3. RACK1 might suppress cardiomyocyte apoptosis aftermyocardial ischemia/reperfusion, providing a novel molecular target for the therapy of ischemia heart disease. PMID: 26659395
    4. this study shows that varicocoele downregulates AR expression, upregulates GPER and RACK1. This regulation of AR may play an important role in testicular dysfunction. PMID: 23836701
    5. Differences in RACK1 levels/localization account for, in part, differential proliferative responses to NPY(1-36) and PYY(1-36) in SHR vs. WKY preglomerular vascular smooth muscle cells. PMID: 23303411
    6. CLCA splicing isoform is associated with adhesion through beta1-integrin and its scaffolding protein PMID: 23297403
    7. RACK1 modulates proliferation of preglomerular microvascular smooth muscle cells and glomerular mesangial cells, likely via the inositol-1,4,5-trisphosphate receptor/calcium/calmodulin pathway. PMID: 21844488
    8. BDNF expression is epigenetically regulated via the scaffolding protein RACK1 PMID: 20410295
    9. NMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1. PMID: 11943848
    10. in astrocytes, RACK1 and PKC-betaII synchronous relocation is not essential for relocation of PKC-betaII to the plasma membrane PMID: 15042584
    11. WD40 domain-mediated homo- and heterodimerization of RACK1 mediate the formation of a transient signaling complex that includes the NMDAR, a G protein and Fyn PMID: 15140893
    12. TCDD activates protein kinase C-betaII via RACK-1 in an aryl hydrocarbon receptor-dependent manner. PMID: 16797713
    13. RACK1 binds to the BK(Ca) channel and it may form part of a BK(Ca)-channel regulatory complex in vascular smooth muscle. PMID: 17166942
    14. analysis showed significant downregulation of RACK1 expression in the rat model of monocrotaline-induced PAH but not in pulmonary arterial smooth muscle cells from PAH patients PMID: 17515463
    15. RACK1 tethers the ERK pathway core kinases and channels signals from upstream activation by integrins to downstream targets at focal adhesions. PMID: 17908799
    16. Activation of adenylate cyclase by PACAP(1-38) results in the release of RACK1 from the NMDA receptor and Fyn. PMID: 12524444

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Perikaryon. Cell projection, dendrite.
  • 蛋白家族：
    WD repeat G protein beta family, Ribosomal protein RACK1 subfamily
  • 数据库链接：

    KEGG: rno:83427

    STRING: 10116.ENSRNOP00000003334

    UniGene: Rn.55487