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  4. Recombinant Rat GTPase IMAP family member 5 (Gimap5), partial

Recombinant Rat GTPase IMAP family member 5 (Gimap5), partial

  • 货号：
    CSB-YP812990RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP812990RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP812990RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP812990RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP812990RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gimap5
  • Uniprot No.：
  • 别名：
    Gimap5; Ian4; Ian4l1; Iddm1; Lyp; GTPase IMAP family member 5; Immunity-associated nucleotide 4 protein; IAN-4; Immunity-associated nucleotide 4-like 1 protein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for mitochondrial integrity and T-cell survival. May contribute to T-cell quiescence.; Plays a role in T lymphocyte development and the optimal generation of CD4/CD8 double-positive thymocytes. Inhibitor of GSK3A, possibly by sequestering GSK3A in cytoplasmic vesicles and impairing its translocation to the nucleus. Consequently, impairs GSK3A-dependent transcriptional program and regulation of the DNA damage response occurring during T cells proliferation. Required for the survival of peripheral T cells, natural killer (NK) and NK T-cell development and the maintenance of normal liver function. Promotes the survival of quiescent T-cells. May regulate Ca(2+) homeostasis by modulating lysosomal Ca(2+) stores, preventing its accumulation in the absence of T cell activation. May play a role in mitochondrial DNA segregation in hematopoietic tissues.
  • 基因功能参考文献：
    1. These findings confirm a critical albeit differential role of T-cell lymphopenia in the susceptibility to organ-specific autoimmune responses. PMID: 26740263
    2. Data indicate that reduced Ca2+ influx in GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5)-deficient T-cells is associated with the inability of their mitochondria to sequester Ca2+ following capacitative entry. PMID: 23098229
    3. results suggest that lack of the Gimap5 protein in the DR.(lyp/lyp) congenic rat was associated with impaired expression of the entire family of Gimap genes and may regulate T cell homeostasis in the peripheral lymphoid organs. PMID: 21925515
    4. Obesity and type 2 diabetes in the BioBreeding diabetes-resistant (BBDR) rat result in islet expansion associated with protection from T1D in female but not male BBDR(lepr-/lepr-,Gimap5-/Gimap5-) congenic rats. PMID: 19996157
    5. diabetes-prone BB rat carries a frameshift mutation in Ian4; propose that Ian4 is identical to Iddm1 [Ian4] PMID: 12031988
    6. Lymphopenia in the BioBreeding rat model of spontaneous autoimmune diabetes is due to a frameshift deletion in Ian5 (immune-associated nucleotide-related gene 5) that results in truncation of a significant portion of the protein. PMID: 12097339
    7. Ian4 is a tissue-specific regulator of mitochondrial integrity and is required for T cell survival PMID: 12930893
    8. mechanisms by which the Ian5 frameshift mutation affects T cell selection, differentiation and maturation without organ-specific autoimmunity PMID: 14624755
    9. in addition to their role in apoptosis, IAN family members may also play a role in regulating the T cell activation state through modulation of TCR signaling strength. PMID: 15307172
    10. Gimap5 functions to promote both T cell survival and quiescence PMID: 16584774
    11. These findings suggest that GIMAP5 regulates T lymphocyte survival by mechanisms that operate upstream of mitochondria. PMID: 17655828
    12. GIMAP5 is an important regulator of calcium response in T lymphocytes and impaired calcium signaling might underlie spontaneous apoptosis of gimap5(lyp/lyp) T cells. PMID: 19007993
    13. results show that spontaneous type 1 diabetes is controlled by at least two genetic loci 7 Mb apart on rat chromosome 4 PMID: 19351909

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  • 相关疾病：
    Defects in Gimap5 are the cause of insulin-dependent diabetes and T-cell lymphopenia. A frameshift mutation results in a truncated protein.
  • 亚细胞定位：
    Lysosome membrane; Single-pass type IV membrane protein. Endosome, multivesicular body membrane; Single-pass type IV membrane protein. Endosome membrane; Single-pass type IV membrane protein.
  • 蛋白家族：
    TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, AIG1/Toc34/Toc159-like paraseptin GTPase family, IAN subfamily
  • 组织特异性：
    Primarily expressed in spleen, heart, lung and intestine and, at lower levels, in kidney, stomach and muscle. Expressed in thymus and lymph nodes (at protein level). In the spleen, expressed in periarteriolar lymphatic sheets. Isoform 2: Expressed at high
  • 数据库链接：

    KEGG: rno:246774

    STRING: 10116.ENSRNOP00000055929

    UniGene: Rn.83549