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  4. Recombinant Rat GTP cyclohydrolase 1 (Gch1)

Recombinant Rat GTP cyclohydrolase 1 (Gch1)

  • 中文名称：
    大鼠Gch1重组蛋白
  • 货号：
    CSB-YP009317RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Gch1重组蛋白
  • 货号：
    CSB-EP009317RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Gch1重组蛋白
  • 货号：
    CSB-EP009317RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Gch1重组蛋白
  • 货号：
    CSB-BP009317RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Gch1重组蛋白
  • 货号：
    CSB-MP009317RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gch1
  • Uniprot No.：
  • 别名：
    Gch1; Gch; GTP cyclohydrolase 1; EC 3.5.4.16; GTP cyclohydrolase I; GTP-CH-I
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    12-241
  • 氨基酸序列
    GFPERELPR PGASRPAEKS RPPEAKGAQP ADAWKAGRPR SEEDNELNLP NLAAAYSSIL RSLGEDPQRQ GLLKTPWRAA TAMQFFTKGY QETISDVLND AIFDEDHDEM VIVKDIDMFS MCEHHLVPFV GRVHIGYLPN KQVLGLSKLA RIVEIYSRRL QVQERLTKQI AVAITEALQP AGVGVVIEAT HMCMVMRGVQ KMNSKTVTST MLGVFREDPK TREEFLTLIR S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence.
  • 基因功能参考文献：
    1. Reduced GCH1 levels in hyperoxia were due, in part, to increased degradation by the ubiquitin-proteasome system PMID: 28399119
    2. Pretreatment with a Gch1 antagonist produced a substantial dose related inhibition of pain behaviors. PMID: 25783971
    3. GCH1 levels of nerve cells in spinal dorsal horn was elevated after chronic sciatic nerve injury. PMID: 23515624
    4. GCH1 interacts with proteins in an organ dependant manner and EIF3I might be a general regulator of GCH1. PMID: 22479495
    5. The N-terminal peptide of mammalian GTP cyclohydrolase I is an autoinhibitory control element and contributes to binding the allosteric regulatory protein GFRP. PMID: 21163945
    6. Following kainic acid there is an increase in striatal GCH1 mRNA, protein, and activity. PMID: 12297263
    7. Dexamethasone induces cyclohydrolase 1 and CAT-2 exression in lung. PMID: 12716655
    8. GTP cyclohydrolase 1 inhibition decreases relaxation and increases blood pressure in rats. PMID: 12855421
    9. Co-expression of GTP cyclohydrolase 1 and iNOS may indicate a self-protective mode of action in osteoblasts against the harmful stress under microgravity. PMID: 15033774
    10. Estrogen modulation of GTP cyclohydrolase I gene expression was dependent on the dose, mode of administration, and brain region. PMID: 15223360
    11. GTCPCH inhibition by DAHP (2,4-diamino-6-hydroxypyrimidine) requires GTPCH feedback regulatory protein PMID: 15292175
    12. conformational analysis of GTP cyclohydrolase I binding GFRP, its feedback regulatory protein PMID: 15448133
    13. Coexpression of Th, Gch, aromatic-L-amino acid-decarboxylases, and VMAT-2 supports long-term biochemical and behavioral correction of a rat model of Parkinson disease. PMID: 15684695
    14. The role of GTP hydrolase I in the production of nitric oxide during heart graft rejection in rats is reported. PMID: 16000090
    15. Increased blood flow causes vascular upregulation of eNOS and GTPCH I. Induction of GTPCH I increases BH4 production, thereby optimizing generation of NO by eNOS and adaptive, vasorelaxant response required in sustaining increased blood flow. PMID: 16199476
    16. Sp1 and Sp3 are found associated with native proximal promoter of cyclic AMP (cAMP)-dependent GTP cyclohydrolase I in PC12 cells but are not recruited to the promoter during cAMP-dependent transcription. PMID: 18004997
    17. GCH-1 activity is regulated either positively by phosphorylation S51, S72, T85, T91, T103, and S130, or negatively at T231. PMID: 19762783

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    GTP cyclohydrolase I family
  • 数据库链接：

    KEGG: rno:29244

    STRING: 10116.ENSRNOP00000014821

    UniGene: Rn.28195