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  4. Recombinant Rat Fibroblast growth factor 23 (Fgf23)

Recombinant Rat Fibroblast growth factor 23 (Fgf23)

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  • 中文名称：
    大鼠Fgf23重组蛋白
  • 货号：
    CSB-EP008629RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fgf23Fibroblast growth factor 23; FGF-23
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    29.5kDa
  • 表达区域：
    25-251aa
  • 氨基酸序列
    YSDTSPLLGSNWGSLTHLYTATARNSYHLQIHRDGHVDGTPHQTIYSALMITSEDAGSVVIIGAMTRRFLCMDLRGNIFGSYHFSPENCRFRQWTLENGYDVYLSPKHHYLVSLGRSKRIFQPGTNPPPFSQFLARRNEVPLLHFYTARPRRHTRSAEDPPERDPLNVLKPRPRATPIPVSCSRELPSAEEGGPAASDPLGVLRRGRGDARRGAGGTDRCRPFPRFV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    To produce recombinant Rat Fgf23 protein, a well-established recombinant DNA technology is the key. A DNA template of Fgf23 was constructed with N-terminal 6xHis tag using the technique. Once the template was made, the recombinant Rat Fgf23 protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 25-251aa of the Rat Fgf23. The purity of this recombinant is 90% determined by SDS-PAGE.

    FGF23 is a protein coding gene that encodes Fibroblast growth factor 23. According to some studies, FGF23 may have the following features.
    FGF23 induces left ventricular hypertrophy. Targeted ablation of Fgf23 demonstrates an important physiological role of FGF23 in phosphate and vitamin D metabolism. FGF23 is a hormone that regulates phosphate metabolism—a unique biological feature of FGF23. Klotho converts the canonical FGF receptor into a specific receptor for FGF23. The rat parathyroid gland is a target organ of FGF23. Mutations in the FGF23 gene cause autosomal dominant hypophosphatemic rickets, possibly by preventing the proteolytic cleavage of FGF23 and enhancing its biological activity. Parathyroid hormone and FGF23 target the kidneys and cause phosphateuria. FGF23 also acts on the parathyroid glands to reduce PTH expression, but in chronic kidney disease.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Regulator of vitamin-D metabolism. Negatively regulates osteoblasts differentiation and matrix mineralization. Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL.
  • 基因功能参考文献：
    1. Increases in plasma erythropoietin and erythropoietin receptor activation are mechanisms implicated in the increase of plasma FGF23 in acute kidney injury. PMID: 29395333
    2. In chronic kidney disease (CKD) rat models, FGF23 mRNA is expressed in the kidney, and the FGF23 protein is expressed at high levels in osteopontin-positive renal tubule epithelium cells likely via TGFbeta1 stimulation. FGF23 produced in the kidney might contribute to P metabolism in subjects with CKD. PMID: 29518087
    3. High FGF23 expression is associated with cardiac hypertrophy. PMID: 28339837
    4. The only direct contribution of the injured kidney to circulating FGF23 levels in uremia appears to be reduced renal extraction of bone-derived FGF23. PMID: 28341272
    5. Osteoblast Fgf23 transcription is upregulated by increase in the cytosolic Ca(2+) activity. Fgf23 transcription is decreased by Orai inhibitors and Orai1 silencing. Fgf23 transcription is lowered by NFkappaB inhibitors. PMID: 26631141
    6. EPO dependent regulation pathway of FGF23 gene expression PMID: 29073196
    7. can directly stimulate hepatic secretion of inflammatory cytokines PMID: 27457912
    8. dietary Mg deficiency causes a rapid increase in circulating levels of FGF23 and renal 24(OH)ase mRNA levels PMID: 27624533
    9. phosphate directly enhances Fgf23 transcription without affecting the stability of Fgf23 messenger RNA. PMID: 25792238
    10. Fgf23 gene transcription was abolished by the actin microfilament-disrupting agent cytochalasin B, as well as by the inhibition of actin-regulating Rac1/PAK1 signaling. PMID: 26878191
    11. Although the molecular link between the cardiac lesion and circulating Fgf23 concentrations remains to be identified, our study has uncovered a novel heart-bone-kidney axis PMID: 25858796
    12. In conclusion, PRL was responsible for the lactation-induced mucosal adaptations, which were associated with compensatory increase in FGF-23 expression probably to prevent calcium hyperabsorption. PMID: 26657069
    13. Data indicate that serum fibroblast growth factor 23 (FGF-23) levels independently correlated with bone volume parameters in rats with experimentally induced chronic kidney disease (CKD). PMID: 26186634
    14. fibroblast growth factor 23 is increased by intravenous phosphate loading in uremic rats PMID: 24625659
    15. The polycystic kidney produces FGF23 but is resistant to its action. PMID: 24402093
    16. FGF23 enhances phosphate-induced vascular calcification by promoting osteoblastic differentiation involving the ERK1/2 pathway in the absence of Klotho deficiency. PMID: 24088960
    17. Ca is not a regulator of acute changes in FGF23 secretion. PMID: 24801007
    18. data suggest that inhibition of FGFR signaling following administration of either pan-FGFR inhibitor or MEK inhibitor interferes with the FGF23 pathway, predisposing animals to hyperphosphatemia PMID: 23872713
    19. In non-iron depleted normal and uremic rats a single high dose of either of two intravenous iron preparations, iron isomaltoside 1000, and ferric carboxymaltose, had no effect on plasma levels of iFGF23 and phosphate for up to seven days. PMID: 24373521
    20. Mg deficiency increases serum FGF-23 levels. PMID: 23608165
    21. a direct and an indirect effect of parathyroid hormone on FGF23 secretion, the latter through changes in calcitriol concentrations PMID: 21525854
    22. late-onset ADHR is the product of gene-environment interactions whereby the combined presence of an Fgf23-stabilizing mutation and iron deficiency can lead to ADHR PMID: 22006328
    23. FGF23 normalizes serum phosphate and decreases 1,25-dihydroxyvitamin D levels in early-stage chronic kidney disease. PMID: 20844473
    24. Serum FGF23 increased in uremic rats treated with paricalcitol but not those treated with cinacalcet. PMID: 20200094
    25. because of a downregulation of the Klotho-FGFR1c receptor complex, an increase of circulating FGF23 does not decrease parathyroid hormone levels in established chronic kidney disease. PMID: 20016468
    26. Data indicate that cleavage at the RXXR motif abrogates FGF23 activity by removing the binding site for the binary FGFR-Klotho complex in the C-terminal region of FGF23, and by generating an endogenous inhibitor of FGF23. PMID: 19966287
    27. a feedback loop exists among serum phosphorus, 1alpha,25(OH)(2)D(3), and FGF-23, in which the novel phosphate-regulating bone-kidney axis integrated with the parathyroid hormone-vitamin D(3) axis in regulating phosphate homeostasis PMID: 15531762
    28. rat UMR-106 osteoblast-like cells were treated with 1,25(OH)(2)D(3) resulted in a dose- and time-dependent stimulation of FGF23 mRNA concentrations. PMID: 16020653
    29. Mineralized tissue cells are a principal source of Fgf23. PMID: 17350357
    30. FGF23 suppressed both parathyroid hormone (PTH) secretion and PTH gene expression PMID: 17992255
    31. These results suggest that the N- and C-terminal domains of FGF23 are responsible for association with cognate FGF receptors and Klotho, respectively, and that these interactions are indispensable for FGF23 activity. PMID: 18442315
    32. Results show that the elevated plasma FGF23 levels in uremic rats reflect the increased expression of FGF23 in bone. PMID: 19339809
    33. estrogens regulate PTH indirectly, possibly through FGF23 PMID: 19628670

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 组织特异性：
    Expressed in the parathyroid.
  • 数据库链接：

    KEGG: rno:170583

    STRING: 10116.ENSRNOP00000026888

    UniGene: Rn.154421