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  4. Recombinant Rat Excitatory amino acid transporter 1 (Slc1a3), partial

Recombinant Rat Excitatory amino acid transporter 1 (Slc1a3), partial

  • 中文名称：
    大鼠Slc1a3重组蛋白
  • 货号：
    CSB-YP021434RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Slc1a3重组蛋白
  • 货号：
    CSB-EP021434RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Slc1a3重组蛋白
  • 货号：
    CSB-EP021434RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Slc1a3重组蛋白
  • 货号：
    CSB-BP021434RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Slc1a3重组蛋白
  • 货号：
    CSB-MP021434RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Slc1a3; Eaat1Excitatory amino acid transporter 1; Glial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; GLAST; GLAST-1; Solute carrier family 1 member 3
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate.
  • 基因功能参考文献：
    1. This study showed an increased expression of glial (GLAST and GLT-1) after paraoxon exposure. PMID: 27769869
    2. EAAT1 is regulated by CaMKII signaling and is a critical driver of constitutive glutamate uptake by EAAT1. PMID: 27889915
    3. Chronic postnatal stress led to increased Glast expression in hippocampus and frontal cortex. PMID: 26037264
    4. EAAT1 most likely mediates Na(+)-dependent high-affinity L-Glutamate (L-GLU) transport at the inner blood retinal barrier (BRB) and appears to take part in L-Glu elimination from the retina across the inner BRB PMID: 26133720
    5. findings suggest that NF-kappaB is a critical positive regulator of EAAT1, mediating the stimulatory effects of EGF, whereas YY1 is a negative regulator of EAAT1 with HDACs as co-repressors PMID: 26269591
    6. proximity between transmembrane domain 2 and hairpin loop 1 of the glutamate transporter EAAT1 PMID: 24692063
    7. During the latent period in this model of temporal lobe epilepsy, EAAT1 mRNA expression is down regulated in the cerebral cortex. PMID: 23392471
    8. Results suggest that an impairment of astrocytic ascorbate-release may exacerbate neuronal dysfunction in neurodegenerative disorders and acute brain injury in which excitotoxicity and/or GLAST deregulation have been implicated. PMID: 22886112
    9. We found a continuous raised hippocampal glutamate and an up-regulation of GLAST in hippocampus of aged rats associated with isoflurane-induced learning/memory impairment PMID: 23070469
    10. Close functional similarities of the GLAST/EAAT-1 promoter regions in man and rat exist which further point to a species-specific function of the GLAST/EAAT-1 3'-UTR in constitutive and regulated GLAST/EAAT-1 expression. PMID: 22252783
    11. Significantly increased expression of glutamine synthetase and glutamate aspartate transporter at 40 mmHg pressure was observed in Muller cells. PMID: 22134673
    12. deficient glial glutamate transporters and glutamine synthetase significantly attenuate GABAergic synaptic strength in the spinal dorsal horn PMID: 22339645
    13. These results indicate that GLT-1a participated in the brain ischemic tolerance induced by cerebral ischemic preconditioning in rats. PMID: 21971915
    14. Glast1a was present in the cerebellar Bergmann glia, and weaker labelling was evident in the retinal Muller cells. GLAST1a is differentially targeted to some cellular compartments such as the end feet of the Muller cells. PMID: 17179860
    15. Glutamate transporter GLAST is downregulated in hippocampus and cerebral cortex following subarachnoid hemorrhage. PMID: 21233495
    16. Results indicate a transient decrease in GLAST glutamate transporter RNA expression during the early stages of a spatial learning task. PMID: 20521381
    17. GLAST can rapidly and specifically respond to changes in the cellular environment thus potentially helping to fine-tune the functions of astrocytes. PMID: 19440835
    18. GLAST-1 may regulate glutamate signalling in bone, consistent with its operation in the central nervous system PMID: 12440940
    19. EAAT1 has a role in myocardial glutamate metabolism PMID: 15242733
    20. Glia modulate the expression of GLAST glutamate transporters in neurons, via at least two distinct mechanisms PMID: 15337311
    21. EAAT1 regulation by thyroid hormone may facilitate increased metabolic demands of the cardiomyocyte during hyperthyroidism and impact cardiac function in hyperthyroidism. PMID: 15615843
    22. we report the identification of five splice variants of the 5'-leader sequence of rat GLT-1 which contain varying numbers of upstream open reading frames and encode putative GLT-1 proteins, and are expressed in a brain region-specific manner. PMID: 15661376
    23. While most glia in the NG2 lineage preserve a certain amount of voltage-gated ion conductances, mature GLAST(+) astrocytes are electrophysiologically passive. PMID: 16093329
    24. GLAST downregulation, mediated by tumor necrosis factor-alpha, has a role in autoantigen-specific T cells inhibition of glutamate uptake in astrocytes PMID: 16123171
    25. Correlation of post-whole cell recording immunohistochemistry for GLAST and the spread of injected dye from the recorded cell with the measured electrophysiological phenotype to quantify cell coupling of astrocytes. PMID: 16775204
    26. GLAST in supporting cells is responsible for transmitter uptake at IHC afferent synapses. (Glutamate-aspartate transporter GLAST) PMID: 16855093
    27. GLAST was expressed primarily in epithelial cells, cells of the macrophage-lineage, lymphocytes, fat cells, interstitial cells, and salivary gland acini PMID: 16868771
    28. These age-related alterations in Glast and glutamate regulation may be key contributors to the increased susceptibility of the aged brain to excitotoxic insults such as stroke and hypoxia. PMID: 16959378
    29. These findings implicate the GLAST-NHERF1 complex in the regulation of glutamate homeostasis in astrocytes. PMID: 17048262
    30. Overall, in tissue sections [(3)H]4MG proved unsuitable for studying the autoradiographic localization of EAATs apparently due to its inability to selectively discriminate Na(+)-dependent binding to Glu transporters. PMID: 17590480
    31. GLAST cytoskeletal anchoring determines susceptibility to brain damage and identifies the role for GFAP PMID: 17684014
    32. Glutamate transport does not appear to change linearly with increased IOP; A pressure threshold exists, above which Muller cell GLAST function is compromised PMID: 18028233
    33. Data show that GLAST expression is decreased in the hippocampal CA1 region in rats with global cerebral ischemia with or without epilepsy. PMID: 18671901
    34. In the hippocampus of group II metabotropic glutamate receptor GluR3-deficient mice, glutamate transporters EAAT1 and EAAT2 are decreased. PMID: 18720515
    35. The alpha1-alpha 3 subunits of Na,K-ATPase were detected, as were the glutamate transporters GLAST and GLT-1, demonstrating that glutamate transporters copurify with Na,K-ATPases. PMID: 19553454
    36. InHO and tMCAO are associated with expression of EAAT1, EAAT2, EAAT3, and TACE, consistent with an active role in the genesis of ischemic protection. PMID: 19717015

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A3 subfamily
  • 组织特异性：
    Detected in brain and cerebellum. Both isoform GLAST-1 and GLAST-1A are expressed in bone and brain. In brain isoform GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum.
  • 数据库链接：

    KEGG: rno:29483

    STRING: 10116.ENSRNOP00000022319

    UniGene: Rn.34134