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  4. Recombinant Rat Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1)

Recombinant Rat Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1)

  • 货号：
    CSB-YP013409RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013409RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013409RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013409RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013409RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Map2k1; Mek1; Prkmk1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; EC 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-393
  • 氨基酸序列
    PKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELLFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA ASI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
  • 基因功能参考文献：
    1. Formalin injection with and without short-term (<10 min) general isoflurane anesthesia induced the same level of phosphorylated ERK expression in spinal cord. PMID: 26272725
    2. Constitutive activation of the mitogen-activated protein kinase kinase 1 can promote ERK1/2 expression. PMID: 23096919
    3. nocodazole increases the ERK(Map2k1) activity to enhance Dusp1(MKP-1) expression which inhibits p38 activation induced by TNF-alpha PMID: 22294037
    4. ERK MAPK was found to be involved in the release of the chemokine MCP-1 in thermal injury. PMID: 21263381
    5. essential role of the MEK/ERK/AP-1 pathway in the disruption of actin cytoskeleton and cell spreading PMID: 20942268
    6. Findings indicate that MEK-1 is a key effector of the HuR-elicited anti-apoptotic programme in IECs. PMID: 20001965
    7. PAK1 phosphorylation of Map2k1 regulates fibronectin-stimulated MAPK activation. PMID: 12876277
    8. MEK1 was found to be crucial for this response as its inhibition abolished the prosurvival properties of bFGF. PMID: 14963006
    9. activation of MEK1 is required for obtaining neuronal phenotype in neural progenitor cells PMID: 17202850
    10. Exogenous CO prevents transplant-induced hepatic ischemia reperfusion injury by down-regulating MEK1 activity. PMID: 18006605
    11. Up-regulation of P-TEFb by the Mek1 signaling pathway contributes to stimulated transcription elongation of immediate early genes in neuroendocrine cells. PMID: 18086894
    12. Activation of MEK1 in intestinal epithelial cells is sufficient to induce an EMT (epithelial-to-mesenchymal transition) associated with tumor invasion and metastasis. PMID: 19462441

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
  • 数据库链接：

    KEGG: rno:170851

    STRING: 10116.ENSRNOP00000013933

    UniGene: Rn.5850