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  4. Recombinant Rat Double-stranded RNA-specific editase 1 (Adarb1)

Recombinant Rat Double-stranded RNA-specific editase 1 (Adarb1)

  • 中文名称：
    Recombinant Rat Double-stranded RNA-specific editase 1(Adarb1),Yeast
  • 货号：
    CSB-YP001325RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Double-stranded RNA-specific editase 1(Adarb1),Yeast
  • 货号：
    CSB-EP001325RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Double-stranded RNA-specific editase 1(Adarb1),Yeast
  • 货号：
    CSB-EP001325RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Double-stranded RNA-specific editase 1(Adarb1),Yeast
  • 货号：
    CSB-BP001325RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Double-stranded RNA-specific editase 1(Adarb1),Yeast
  • 货号：
    CSB-MP001325RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Adarb1
  • Uniprot No.：
  • 别名：
    Adarb1; Red1; Double-stranded RNA-specific editase 1; EC 3.5.4.37; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-711
  • 氨基酸序列
    MDIEDEENMS SSSIDVKENR NLDNMPPKDS STPGPGEGIP LSNGGGGSTS RKRPLEEGSN GHSKYRLKKR RKTPGPVLPK NALMQLNEIK PGLQYMLLSQ TGPVHAPLFV MSVEVNGQVF EGSGPTKKKA KLHAAEKALR SFVQFPNASE AHLAMGRTLS VNTDFTSDQA DFPDTLFNGF ETPDKSEPPF YVGSNGDDSF SSSGDVSLSA SPVPASLTQP PLPIPPPFPP PSGKNPVMIL NELRPGLKYD FLSESGESHA KSFVMSVVVD GQFFEGSGRN KKLAKARAAQ SALATVFNLH LDQTPSRQPV LSEGLQLHLP QVLADAVSRL VLGKFSDLTD NFSSPHARRK VLSGVVMTTG TDVKDAKVIS VSTGTKCING EYMSDRGLAL NDCHAEIISR RSLLRFLYAQ LELYLNNKED QKKSIFQKSE RGGFRLKDTV QFHLYISTSP CGDARIFSPH EPVLEGMAPD SHQLTEPADR HPNRKARGQL RTKIESGEGT IPVRSNASIQ TWDGVLQGER LLTMSCSDKI ARWNVVGIQG ALLSIFVEPI YFSSIILGSL YHGDHLSRAM YQRISNIEDL PPLYTLNKPL LSGISNAEAR QPGKAPNFSV NWTVGDTAIE VINATTGKDE LGRPSRLCKH ALYCRWMRVH GKVPPHLLRT KITKPTTYHE SKLAAKEYQA AKARLFTAFI KAGLGAWVEK PTEQDQFSFT P
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can inhibit cell proliferation and migration and can stimulate exocytosis.
  • 基因功能参考文献：
    1. lower levels of ADAR2 in the nucleus of immature neurons than in mature neurons. PMID: 28082424
    2. The JNK1 pathway may be functionally linked to the nutrient-sensing actions of ADAR2-mediated RNA editing in professional secretory cells. PMID: 23139803
    3. Cleaved ADAR2 leads to a decrease or loss of GluR2 editing, which will further result in high Ca2 influx and excitotoxic neuronal death. PMID: 21620933
    4. interaction with RNA transcripts containing selective and nonselective editing sites PMID: 12810917
    5. the nucleolar localization of ADAR2 represents an important mechanism by which RNA editing can be modulated by the sequestration of enzymatic activity from potential RNA substrates in the nucleoplasm PMID: 14612560
    6. both sequence and structural elements are required to define adenosine moieties targeted for specific ADAR2-mediated deamination PMID: 14660658
    7. results implicate the carboxy-terminal domain of the large subunit of RNA polymerase II in site-selective RNA editing by ADAR2 and in coordination of editing with alternative splicing. PMID: 16440002
    8. RNA structure-dependent recognition by the two double-stranded RNA binding motifs of rat ADAR2. PMID: 16472753
    9. ADAR2 double-stranded RNA binding motifs serve differential roles in RNA dimerization in RNA eduting. PMID: 16682559
    10. RNA editing by ADAR2 in pancreatic islets and beta-cells is metabolically regulated by nutritional and energy status PMID: 16956888

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus.
  • 组织特异性：
    Brain and peripheral tissues.
  • 数据库链接：

    KEGG: rno:25367

    STRING: 10116.ENSRNOP00000001642

    UniGene: Rn.172406