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  4. Recombinant Rat Diacylglycerol kinase gamma (Dgkg)

Recombinant Rat Diacylglycerol kinase gamma (Dgkg)

  • 中文名称：
    大鼠Dgkg重组蛋白
  • 货号：
    CSB-YP006836RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Dgkg重组蛋白
  • 货号：
    CSB-EP006836RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Dgkg重组蛋白
  • 货号：
    CSB-EP006836RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Dgkg重组蛋白
  • 货号：
    CSB-BP006836RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Dgkg重组蛋白
  • 货号：
    CSB-MP006836RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dgkg; Dagk3Diacylglycerol kinase gamma; DAG kinase gamma; EC 2.7.1.107; 88 kDa diacylglycerol kinase; Diglyceride kinase gamma; DGK-gamma
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-788
  • 氨基酸序列
    MSDGQWVCLS SEEFDQLQKY SEYSSKKIKD VLAEFNEGGS LKQYDPHKPI SYDVFKLFMR AYLEVDLPQP LSTNLFLAFS QKPRQETPDH PKEGASSSEP NVSDSNAEST AKADAACAPD TESKPIKTQV PSEELEAAAP WGEPNAPASS SDAPIVYLKD VVCYLSLMET GRPQDKLEFM FRLYDSDENE LLDQAELDQI VSQMLHVAQY LEWDPTELRP ILKEMLQGMD YNKDGFVSLE EWVSGGMTTI PLLVLLGMDD SASKGDGRHA WTLKHFKKPT YCNFCHIMLM GVRKQGLCCI YCKYAVHQRC VSNSIPGCVK TYSKAKRSGE VMQHAWVEGN SSVKCDRCHK SIKCYQSVTA RHCVWCRMTF HRKCELSTAC DGGELKDHIL LPTSIYPVTR DRQAGKSDSG AAAKGELVMQ YKIIPSPGTH PLLVLVNPKS GGRQGERILQ KFHYLLNPKQ VFNLDKGGPT PGLNFFQDTP DFRVLACGGD GTVGWILDCI DKANFTKHPP VAVLPLGTGN DLARCLRWGG GYEGGSLTKI LKEIEQSPLV MLDRWYLEVM PREEVENGDQ VPYNIMNNYF SIGVDASIAH RFHVMREKHP EKFNSRMKNK LWYFEFGTSE TFAATCKKLH DHIELECDGV EVDLSNIFLE GIAILNIPSM YGGTNLWGET KKNRAVIRES RKSVTDPKEL KCCVQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVTIRTKKL LPMQVDGEPW MQPPCMIKIT HKNQAPMMMG PPQKSSFFSL RRKSRSKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has no apparent specificity with regard to the acyl compositions of diacylglycerol. Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination.
  • 基因功能参考文献：
    1. Knockdown of Dgkg, but not DGK-alpha, inhibited degranulation in mast cells. PMID: 24513282
    2. In brain, DGKgamma showed sustained expression throughout the postnatal developmental periods. And In the hippocampal pyramidal cell, Dgkg localized in the cytosol. PMID: 16019106
    3. localization and activation of the functionally correlated kinases, gammaPKC and DGKgamma, are spatio-temporally orchestrated by their direct association and phosphorylation, contributing to subtype-specific regulation of DGKgamma and DAG signaling PMID: 16905533
  • 亚细胞定位：
    Membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Eukaryotic diacylglycerol kinase family
  • 组织特异性：
    Expressed specifically in brain. Highly expressed in cerebellar Purkinje cells (at protein level).
  • 数据库链接：

    KEGG: rno:25666

    STRING: 10116.ENSRNOP00000002452

    UniGene: Rn.10544