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  4. Recombinant Rat Aminopeptidase N (Anpep), partial

Recombinant Rat Aminopeptidase N (Anpep), partial

  • 中文名称：
    Recombinant Rat Aminopeptidase N(Anpep),partial
  • 货号：
    CSB-EP001827RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP001827RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Anpep.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP001827RA could indicate that this peptide derived from E.coli-expressed Rattus norvegicus (Rat) Anpep.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    AnpepAminopeptidase N; AP-N; rAPN; EC 3.4.11.2; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Kidney Zn peptidase; KZP; Microsomal aminopeptidase; CD antigen CD13
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    65.8kDa
  • 表达区域：
    33-476aa
  • 氨基酸序列
    YAQEKNRNAENSAIAPTLPGSTSATTSTTNPAIDESKPWNQYRLPKTLIPDSYQVTLRPYLTPNEQGLYIFKGSSTVRFTCNETTNVIIIHSKKLNYTNKGNHRVALRALGDTPAPNIDTTELVERTEYLVVHLQGSLVKGHQYEMDSEFQGELADDLAGFYRSEYMEGGNKKVVATTQMQAADARKSFPCFDEPAMKASFNITLIHPNNLTALSNMLPKDSRTLQEDPSWNVTEFHPTPKMSTYLLAYIVSEFKYVEAVSPNRVQIRIWARPSAIDEGHGDYALQVTGPILNFFAQHYNTAYPLEKSDQIALPDFNAGAMENWGLVTYRESALVFDPQSSSISNKERVVTVIAHELAHQWFGNLVTVDWWNDLWLNEGFASYVEFLGADYAEPTWNLKDLIVLNDVYRVMAVDALASSHPLSSPANEVNTPAQISELFDSITY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.
  • 基因功能参考文献：
    1. Data indicate that fluorogenic substrates can be successfully used to identify aminopeptidase N and to measure their activity in cell lysates. PMID: 26449746
    2. the LAP1 gene (TOR1AIP1) undergoes alternative splicing to originate three LAP1 isoforms (LAP1A, B and C). PMID: 25461922
    3. the anti-oxidant potential of APN in oxidative stress-associated cardiovascular diseases, such as hypertension-induced HF-preserved EF. PMID: 23894332
    4. This study demonistrated that aminopeptidase N were up-regulated ipsilaterally from 6 h to 7 days post ischemia PMID: 22373413
    5. Results suggest that the upregulation of serotonergic activity may be related to a lowered brain dipeptidyl peptidase III, alanyl- and arginyl aminopeptidase activity which may influence the cleavage of their substrates. PMID: 18547641
    6. hypothalamic CD13 plays a role in the regulation of energy metabolism not by means of puromycin-insensitive membrane-bound neutral aminopeptidase enzyme activity. PMID: 20851150
    7. The present study clearly demonstrated that the activities of AlaAP and CysAP in the hippocampus were associated with lateralization in normal rats. PMID: 20096929
    8. We therefore hypothesize that the effect of angiotensin IV on dopamine release in the striatum is mediated via activation of IRAP and/or AP-N, possibly acting as receptors for angiotensin IV. PMID: 17169335
    9. a possible association of the Anpep gene with hypertension in Dahl rat and raise the prospect that increased Anpep may play a mechanistic role in adaptation to high salt. PMID: 17242304
    10. These results demonstrate that expression of neprilysin and aminopeptidase N can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific. PMID: 17952634

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase M1 family
  • 组织特异性：
    Widely distributed throughout the CNS. Particularly abundant in kidney and intestinal microvilli, also detected in lung and liver. Weakly expressed in heart and aorta.
  • 数据库链接：

    KEGG: rno:81641

    STRING: 10116.ENSRNOP00000020002

    UniGene: Rn.11132