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  4. Recombinant Rat Amiloride-sensitive sodium channel subunit alpha (Scnn1a), partial

Recombinant Rat Amiloride-sensitive sodium channel subunit alpha (Scnn1a), partial

  • 中文名称：
    大鼠Scnn1a重组蛋白
  • 货号：
    CSB-YP020848RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Scnn1a重组蛋白
  • 货号：
    CSB-EP020848RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Scnn1a重组蛋白
  • 货号：
    CSB-EP020848RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Scnn1a重组蛋白
  • 货号：
    CSB-BP020848RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Scnn1a重组蛋白
  • 货号：
    CSB-MP020848RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Scnn1a
  • Uniprot No.：
  • 别名：
    Scnn1a; Renac; Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
  • 基因功能参考文献：
    1. Studied the effect and underlining mechanisms of MaR1(maresin 1) in modulating alveolar fluid clearance on LPS-induced acute lung injury; found MaR1 improved epithelial sodium channel (ENaC) and Na,K-adenosine triphosphatase expression and activity. PMID: 28218740
    2. middle ear mucosal expression increased in otitis media with effusion PMID: 25873260
    3. Estradiol signaling through G protein-coupled estrogen receptor selectively activates alveolar ENaC through an effect on channel gating and channel density. PMID: 24097558
    4. Na(+) displays a direct dose-related effect on osteoblasts by affecting differentiation, osteogenic gene expression profile, and ENaC-alpha gene expression. PMID: 22126768
    5. It was found that mRNA deletion forms (a and b), products of alternative splicing of aENaC gene, are absent in the cortex of adult rat kidney. PMID: 22485214
    6. Data indicate that functional ENaC (subunits Scnn1a, Scnn1b, and Scnn1g) may affect firing patterns of magnocellular cells (i.e., large neurons) in supraoptic nucleus and paraventricular nucleus and thus control secretion of vasopressin and oxytocin. PMID: 22045317
    7. Persistent high expression of alphaENaC and increase in apical localization of beta and gammaENaC may contribute to greater retention of sodium in salt-resistant rats on a high-salt diet. PMID: 21423289
    8. emotional stress has an effect on the expression of the alpha-ENaC gene mRNA in the kidney of hypertensive ISIAH rats PMID: 21953222
    9. Hyperaldosteronism and altered expression of an SGK1-dependent sodium transporter in ZDF rats leads to salt dependence of blood pressure. PMID: 20664544
    10. alpha-ENaC protein abundance and ENaC activity are increased in the late pregnant rat, thereby contributing to the positive sodium balance of pregnancy. PMID: 20686170
    11. Expression of epithelial sodium channel alpha subunit is not affected by terbutaline treatment of acute lung injury. PMID: 19460695
    12. ENaC alpha-subunit mRNA showed significantly higher expression than beta- and gamma-subunit mRNAs in isolated rat alveolar type II cells. PMID: 19218112
    13. elucidate key aspects of ENaC trafficking and turnover rates in mammalian kidney epithelial cells PMID: 11773057
    14. are expressed by rat alveolar epithelial type I cells PMID: 11880284
    15. hypoxia-induced inhibition of amiloride-sensitive sodium channel activity is mediated by decreased apical expression of ENaC subunits PMID: 12372821
    16. Proteins or protein-dependent factors within cardiogenic pulmonary oedema fluid induce an alpha-ENaC-independent and amiloride-insensitive apical membrane Na(+) conductance and liquid absorption in the distal lung. PMID: 12381825
    17. inhibitory effect of cAMP-activated CFTR on ENaC is due to a decrease in channel open probability. PMID: 12548398
    18. Ischemic acute renal failure leads to down regulation of pulmonary ENaC, Na,K-ATPase and aquaporin-5. PMID: 12631124
    19. SGK2 and SGK3 mimic the function of SGK1 and are likely to participate in the regulation of ENaC activity. PMID: 12632189
    20. Induction of alphaENaC mRNA may play a permissive role in the enhancement of the early and/or late responses; these effects may be necessary for a full response but do not by themselves promote early changes in urinary Na(+) and K(+) excretion. PMID: 12707381
    21. ATP regulation of ENaC may be involved in the fine-tuning of ENaC activity under physiologic and pathophysiologic conditions PMID: 12876281
    22. Besides mutations Y279A and H282D, which had amiloride binding affinities similar to that of wild-type alpha-ENaC, all other mutations in this region caused changes in the amiloride binding affinity of the channels compared with the wild-type channel. PMID: 12928313
    23. Modulation of ENaC Po by endogenous epithelial serine proteases is potentially important regulator of epithelial Na+ transport, distinct from regulation achieved by hormone-induced plasma membrane insertion of channels. PMID: 12967915
    24. TNF-alpha decreased the expression of the alpha-, beta-, and gamma-subunits of epithelial sodium channel (ENaC) mRNA to 36, 43, and 16% of the controls after 24-h treatment and reduced to 50% the amount of alpha-ENaC protein in lung epithelium PMID: 14514522
    25. ENaCalpha mRNA expression in medullary thick ascending limb but not in collecting ducts is acutely regulated by AVP and hyperosmolality. PMID: 14567502
    26. A heterooligomer composed of alpha-, beta-, and gamma-ENaC may be the molecular basis of the native channels, which are responsible for amiloride-sensitive electrogenic Na+ absorption in rat rectal colon. PMID: 14576089
    27. Results suggest that there are different translational efficiencies for epithelial sodium channel subunits alpha, beta and gamma, and that perinatal processes globally modulate lung mRNA translation. PMID: 14672917
    28. effects of two mutations of the extracellular loop of the alpha-subunit of the ENaC on amiloride-sensitive current; data indicate both tyrosines are important for proper ENaC function and their oxidative modifications contribute to altered ENaC function PMID: 14969999
    29. the increased or sustained expression of ENaC subunits combined with increased apical targeting in the distal tubule, connecting tubule, and collecting duct are likely to play a role in the sodium retention associated with PAN-induced nephrotic syndrome PMID: 15075188
    30. inhibitory effect of [Cl-]i occurred regardless of the presence or absence of net Cl- transport. PMID: 15161604
    31. Alpha-, beta- and gamma-ENaC were extensively labeled in the inner ear. Functional ENaC in the labyrinth may work in concert with other Na+ and K+ transport molecules to regulate endolymph and to maintain homeostasis in the inner ear. PMID: 15219314
    32. SGK1 can stimulate ENaC activity independently of an inhibition of Nedd4-2-mediated channel retrieval PMID: 15234985
    33. IL-1b decreases expression of the ENaC alpha-subunit in alveolar epithelial cells via a p38 MAPK-dependent signaling pathway PMID: 15755725
    34. Steady state renal mRNA of ENaC alpha, beta and gamma were unchanged in liver cirrhosis. PMID: 15956070
    35. ENaC mRNA and protein expression are not increased in the early phase of unilateral puromycin aminonucleoside-induced nephrotic syndrome PMID: 16020936
    36. the binding of active Nedd4-2 to ENaC is a crucial step in the mechanism of ENaC inhibition by progesterone PMID: 16172119
    37. Beta-adrenoceptor stimulation of lung fluid absorption is critically epithelial Na channel(ENaC) dependent, whereas baseline lung fluid absorption seemed less ENaC dependent. PMID: 16258001
    38. This is the first study to report a direct and specific biochemical interaction between F-actin and amiloride-sensitive epithelial sodium channel. PMID: 16356937
    39. both the escape-associated rise in blood pressure as well as upregulation of EnaC and NCC do not require increase in plasma aldosterone PMID: 16449357
    40. rats maintained on a low-Na diet for 7-9 days, the abundance of two forms of the alpha-subunit, with apparent masses of 85 and 30 kDa, increased. PMID: 16554417
    41. Dexamethasone alleviates the downregulation of ENaC expression and activity in TNF-treated alveolar epithelial cells. PMID: 16877633
    42. ENaC and Nedd4-2 are involved in the transition from lung fluid secretion to fluid absorption near term and at birth. PMID: 17693485
    43. ENaC alpha is functionally related to regulatory volume increase and hypertonicity-induced cation channel activation in rat hepatocytes PMID: 17926064
    44. Dietary sodium chloride reduced Scnn1a expression in vascular smooth muscle cells. PMID: 18024548
    45. Immunohistochemistry analyses in rat testis localized ENaC-alpha expressed in the Leydig cell, Sertoli cell, Ap spermatogonium, spermatocyte, spermatid and residual body. PMID: 18298571
    46. Expressed at the apical membrane of the principal cells in rats; a low-Na diet or aldosterone perfusion prevents expression (a 30-kD resolution limit) PMID: 18504317
    47. activation of the NF-kappaB pathway down-regulates SGK1 expression, which might lead to decreased ENaC alpha-subunit expression, ultimately resulting in decreased Na(+) transport PMID: 18586672
    48. Increased alpha-ENaC levels and function are partly dependent upon the early induction of SGK1 expression. PMID: 18615584
    49. support a model in which protons modulate ENaC gating by relieving Na(+) self-inhibition PMID: 18990692
    50. Altered renal expression of Scnn1a in protein-deprived rats. PMID: 19202345

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cell projection, cilium. Cytoplasmic granule. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell projection, cilium, flagellum.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, SCNN1A subfamily
  • 组织特异性：
    Detected in kidney, lung and testis (at protein level). In the testis, detected within the seminiferous tubules but not in the interstitial cells (at protein level).
  • 数据库链接：

    KEGG: rno:25122

    STRING: 10116.ENSRNOP00000061813

    UniGene: Rn.9808